{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:16:38Z","timestamp":1790111798033,"version":"4.0.1"},"reference-count":2,"publisher":"Scientific Scholar","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCAS"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Non-cultured epidermal suspension technique is currently the surgical treatment of choice for vitiligo. Storage of trypsin ethylenediaminetetraacetic acid solution has a stringent requirement. We propose usage of freeze-dried trypsin for the procedure which can be kept in usual refrigerator at 2-8\u00b0C. This can help us to perform the procedure at resource poor settings.<\/jats:p>","DOI":"10.4103\/jcas.jcas_61_22","type":"journal-article","created":{"date-parts":[[2024,2,18]],"date-time":"2024-02-18T08:00:07Z","timestamp":1708243207000},"page":"156-157","source":"Crossref","is-referenced-by-count":2,"title":["Noncultured epidermal suspension procedure of vitiligo surgery using freeze-dried trypsin coated on silicon pellets"],"prefix":"10.25259","volume":"17","author":[{"given":"Sanjiv","family":"Choudhary","sequence":"first","affiliation":[{"name":"Department of Dermatology, All India Institute of Medical Sciences (AIIMS), Nagpur, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suryakant","family":"Hayatnagarkar","sequence":"additional","affiliation":[{"name":"Cryocell India, Aurangabad, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maninder","family":"Kaur","sequence":"additional","affiliation":[{"name":"Cryocell India, Aurangabad, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suman","family":"Patra","sequence":"additional","affiliation":[{"name":"Department of Dermatology, All India Institute of Medical Sciences (AIIMS), Jodhpur, Rajasthan, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2024,4,27]]},"reference":[{"key":"CIT0001-20240429","doi-asserted-by":"crossref","first-page":"59","DOI":"10.4103\/0378-6323.90947","article-title":"Non-cultured epidermal suspension in vitiligo: From laboratory to clinic","volume":"78","author":"Gauthier","year":"2012","journal-title":"Indian J Dermatol Venereol Leprol"},{"key":"CIT0002-20240429","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.bej.2015.09.018","article-title":"Optimizing storage conditions to prevent cold denaturation of trypsin for sequencing and to prolong its shelf life","volume":"105","author":"Ra\u0161kovi\u0107","journal-title":"Biochem Eng J"}],"container-title":["Journal of Cutaneous and Aesthetic Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/10.4103\/JCAS.JCAS_61_22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T07:17:55Z","timestamp":1725088675000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcasonline.com\/noncultured-epidermal-suspension-procedure-of-vitiligo-surgery-using-freeze-dried-trypsin-coated-on-silicon-pellets\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":2,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/jcas.jcas_61_22","relation":{},"ISSN":["0974-2077"],"issn-type":[{"value":"0974-2077","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4]]}}}