{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:31:02Z","timestamp":1787841062627,"version":"build-2784847793"},"reference-count":15,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Intracanal medication is recommended after the debridement of the canal to eliminate bacteria to prevent apical periodontitis. Combining calcium hydroxide (CH) with DJK-5 peptide will improve the penetration of medicament into root canal dentin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>This study was performed to evaluate the cytotoxicity of a combination of DJK-5 peptide with CH on murine fibroblast cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Settings and Design:<\/jats:title>\n                    <jats:p>\n                      This is an\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      experimental study performed using the L929 fibroblast cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>L929 fibroblast cells were grown in Dulbecco\u2019s Modified Eagle\u2019s Medium and administered with DJK-5 peptide at a concentration of 10 \u00b5g\/mL, CH+ saline, CH+ 2% chlorhexidine (CHX), 2% CHX, and CH+ DJK-5 for 24 h and 48 h. Cytotoxicity assessment of the treatments was carried out using the methyl thiazolyl tetrazolium assay and Live\/Dead assay by acridine orange and ethidium bromide dual-fluorescence staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Statistical Analysis:<\/jats:title>\n                    <jats:p>One-way analysis of variance was used for group-wise comparison of cytotoxicity of the test compounds. Pairwise comparison between groups was analyzed by Tukey\u2019s test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The DJK-5-treated group exhibited no cytotoxicity at 24 h and 48 h and exposure time points. Highest cytotoxicity was exerted by the 2% CHX-treated group, which increased when combined with CH, and the cytotoxicity increased with time. Combining the DJK-5 with CH reduced the cytotoxicity when compared to CH or CHX alone.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>At its used 2% concentration, CHX was found to exhibit high cytotoxicity. The least cytotoxicity was found in DJK-5-treated cells, and it also significantly alleviated the cytotoxicity rendered by CH treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jcde.jcde_20_26","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T06:00:23Z","timestamp":1774850423000},"page":"435-439","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Cytocompatibility of calcium hydroxide medicament combined with cationic peptide \u2013 An in vitro study on L929 fibroblast cells"],"prefix":"10.4103","volume":"29","author":[{"given":"Shravan","family":"Kini","sequence":"first","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, Yenepoya Dental College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Praveen","family":"Kumar","sequence":"additional","affiliation":[{"name":"Consultant Endodontist, Jothi Dental Care, Tiruppur, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Harish S.","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, Yenepoya Dental College, Yenepoya (Deemed to be University), Mangalore, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prathap M. 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