{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T13:49:53Z","timestamp":1785764993124,"version":"3.56.0"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Pathogens persisting in the root canals lead to endodontic treatment failure. Understanding their prevalence and antimicrobial susceptibility is essential for improving disinfection and treatment outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To estimate the prevalence of five specific pathogens in failed root canal treatment cases using both culture-based and molecular methods and to evaluate the association between pathogen detection and clinical\/radiographic findings and assess the antimicrobial susceptibility of the isolates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology:<\/jats:title>\n                    <jats:p>\n                      One hundred samples were collected using the absorbent paper point method and analyzed using culture and multiplex quantitative polymerase chain reaction (qPCR) for the detection of\n                      <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Streptococcus mutans<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Fusobacterium nucleatum<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Porphyromonas gingivalis<\/jats:italic>\n                      . Antimicrobial susceptibility testing was performed on culture-positive isolates (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 34). Statistical analysis included the Chi-square test, Fisher\u2019s exact test, and logistic regression.\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05 was considered statistically significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Detection rates were 29% by culture and 82% by qPCR, with\n                      <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                      frequently identified. No statistically significant association was observed between pathogen detection and clinical\/radiographic findings. Isolates were relatively more sensitive to combined antibiotic formulations than individual antibiotics and resistant to calcium hydroxide under test conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Failed root canal treatments demonstrated a high prevalence of persistent pathogens, which are better detected by molecular methods. Observed antimicrobial resistance (AMR), particularly to conventional intracanal medicaments, highlights the growing concern of AMR in endodontic infections and the need for better disinfection strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jcde.jcde_440_26","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:00:21Z","timestamp":1785513621000},"page":"868-874","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Targeted detection and antimicrobial susceptibility of common pathogens of failed root canal treatments: A cross-sectional study"],"prefix":"10.4103","volume":"29","author":[{"given":"Chatura","family":"Mohan","sequence":"first","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 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