{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:21:34Z","timestamp":1788351694670,"version":"build-2803163510"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Context:<\/jats:title>\n                    <jats:p>Human dental pulp stem cell (hDPSC)-derived exosomes may support dentin\u2013pulp regeneration, but free exosomes have limited local retention. Human amniotic membrane hydrogel may serve as a scaffold for exosome delivery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>This study evaluated the effect of exosome-loaded human amniotic membrane (hAM-Exo) hydrogel on hDPSC responses compared with free exosomes under basal and lipopolysaccharide (LPS)-stimulated conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      hDPSCs were treated with free exosomes or hAM-Exo at 0.5%, 1%, and 5%, with or without LPS. Viability, cytocompatibility, migration, vascular endothelial growth factor-A (VEGF-A) secretion, and dentin sialophosphoprotein (DSPP) expression were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, live\/dead, scratch, and enzyme-linked immunosorbent assay (ELISA) assays. Data were analyzed using two-way analysis of variance and\n                      <jats:italic toggle=\"yes\">post hoc<\/jats:italic>\n                      tests.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>hAM-Exo was cytocompatible in all groups and showed a more stable metabolic profile than free exosomes at 96 h. hAM-Exo 1% significantly increased VEGF-A secretion under basal conditions at 48 h. Under LPS stimulation, hAM-Exo better preserved VEGF-A secretion than free exosomes, while hAM-Exo at all concentrations significantly enhanced DSPP expression on days 7 and 14.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>hAM-Exo modulated regenerative-related hDPSC responses under basal and inflammatory conditions compared with free exosomes, supporting scaffold-assisted exosome delivery for cell-free regenerative endodontics.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jcde.jcde_576_26","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:00:18Z","timestamp":1788184818000},"page":"993-998","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Exosome-loaded human amniotic membrane hydrogel modulates regenerative responses of human dental pulp stem cells"],"prefix":"10.4103","volume":"29","author":[{"given":"Hesti Witasari Jos","family":"Erry","sequence":"first","affiliation":[{"name":"Doctoral Study Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia"},{"name":"Department of Conservative Dentistry, Faculty of Dentistry, Universitas YARSI, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anggraini","family":"Margono","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dini Asrianti","family":"Bagio","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dewi Fatma","family":"Suniarti","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indra","family":"Kusuma","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, Universitas YARSI, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hendrik Setia","family":"Budi","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachmat","family":"Mauludin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, Institut Teknologi Bandung, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dodi Valentino","family":"Tambun","sequence":"additional","affiliation":[{"name":"Dental Profession Study Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Normalina","family":"Sandora","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Indonesia Medical Education and Research Institute, Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanaphum","family":"Osathanon","sequence":"additional","affiliation":[{"name":"Center of Excellence in Dental Stem Cell Biology, Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa Rinanda","family":"Amir","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"R1-20260902","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1111\/iej.12954","article-title":"Regenerative endodontics: A comprehensive review","volume":"51","author":"Kim","year":"2018","journal-title":"Int Endod J"},{"key":"R2-20260902","first-page":"9127074","article-title":"Dental pulp stem cell heterogeneity: Finding superior quality \"needles\" in a dental pulpal \"Haystack\" for regenerative medicine-based applications","author":"Kok","year":"2022","journal-title":"Stem Cells Int 2022"},{"key":"R3-20260902","doi-asserted-by":"crossref","first-page":"857066","DOI":"10.3389\/fcell.2022.857066","article-title":"Clinical potential of dental pulp stem cells in pulp regeneration: Current endodontic progress and future perspectives","volume":"10","author":"Kwack","year":"2022","journal-title":"Front Cell Dev 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