{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T17:13:42Z","timestamp":1791825222342,"version":"4.3.4"},"reference-count":36,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,5]]},"abstract":"\n Background:<\/jats:title>\n \n Root canal treatment (RCT) failure occurs due to persistent infections, missed canals, or anatomical complexities, with\n Enterococcus faecalis<\/jats:italic>\n being the predominant microorganism associated with treatment failure. This study aimed to determine the prevalence of\n E. faecalis<\/jats:italic>\n in failed root canal cases and its correlation with clinical parameters.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n A cross-sectional study was conducted on 50 patients aged 18\u201350 years requiring nonsurgical endodontic retreatment. Samples were collected using sterile paper points, gutta-percha, and oral swabs. Clinical data such as age, gender, tooth location, and radiographic findings were documented.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n All samples exhibited polymicrobial infections.\n E. faecalis<\/jats:italic>\n was the most prevalent species predominantly isolated using paper points. Other organisms included\n Streptococcus<\/jats:italic>\n spp.,\n Candida<\/jats:italic>\n spp., and\n Escherichia coli<\/jats:italic>\n . Oral swabs showed lower detection rates for\n E. faecalis<\/jats:italic>\n as compared to other samples for microbial isolation, Gram-positive bacteria (86%) dominated, followed by Gram-negative bacteria (8.3%) and yeast (5%). The findings confirm\n E. faecalis<\/jats:italic>\n as the most prevalent microorganism in failed RCT cases. Its resilience, ability to form biofilms, and resistance to conventional treatment contribute to persistent infections. No significant correlation was observed between bacterial prevalence and demographic factors or tooth position. Differences in microbial detection rates among sampling methods emphasize the importance of multiple sampling approaches for accurate microbial recovery.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n E. faecalis<\/jats:italic>\n is highly prevalent in refractory endodontic infections, underscoring its role in treatment failure. The use of sterile paper points and gutta-percha samples effectively detects intracanal microbes. Advanced molecular techniques may further improve the understanding of endodontic microbiota and help reduce failure rates.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jcde.jcde_871_24","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T01:00:45Z","timestamp":1746493245000},"page":"462-467","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":25,"title":["Prevalence of Enterococcus faecalis in refractory endodontic infections: A microbiological study"],"prefix":"10.4103","volume":"28","author":[{"given":"Jyoti","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Microbiology, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swaty","family":"Jhamb","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manjula","family":"Mehta","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagat","family":"Bhushan","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia Bhonchal","family":"Bhardwaj","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandeep","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"R1-20260423","first-page":"103","article-title":"Outcome of secondary root canal treatment: A systematic review of the literature","volume":"44","author":"Ng","year":"2011","journal-title":"Int Endod J"},{"key":"R2-20260423","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1111\/j.1365-2591.2007.01322.x","article-title":"Outcome of primary root canal treatment: Systematic review of the literature \u2013 Part 1. 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