{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:38:06Z","timestamp":1786649886428,"version":"build-2736575974"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The ongoing infection and transmission of the severe acute respiratory syndrome coronavirus 2019 (SARS-CoV2) has challenged the global health system in the recent past. However, currently, the infection rate has been substantially reduced, but the pandemic showed an inevitability to study the human response to the SARS-CoV2 infection. The core objective of this study is to determine the frequency of individuals in the healthcare workforce, who possess IgG antibodies specific to SARS-CoV-2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A cross-sectional study was conducted at tertiary care hospital of Maharashtra region. Total 300 Health care workers were including in the study. Their blood samples were tested for SARS-CoV-2 IgG antibody using Chemiluminescence methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The study was poised between male and female participants, with 47% and 53% of participants, respectively. The result showed 27% of seroprevalence, among the total participants, where 88% of seropositive participants were diagnosed with COVID-19 using RT-PCR techniques. The current study also illustrated the seroprevalence of the different classes of healthcare workers. Here, technicians and nurses showed a higher rate of seropositivity, 39.02% and 36.36%, respectively. Daily and close contacts with the COVID-19 patients were examined in relation to seropositivity. This study collected the RT-PCR results of the participants and used statistical tests to establish the association of RT-PCR results with seropositivity and symptoms (early and late). RT-PCR showed a significant relationship with early and late symptoms with a\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      value &lt; 0.05. However, seropositivity failed to show a direct significant relationship with the RT-PCR positive results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>This study provides a framework that can be replicated in other communities. Overall, this study examined the seropositivity rate among the HCWs that could assist the hospital management in designing more effective COVID-19 appropriate guidelines.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jfmpc.jfmpc_136_25","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T13:00:39Z","timestamp":1767013239000},"page":"5078-5085","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Seroprevalence of SARS CoV 2 IgG in pandemic frontline healthcare workers"],"prefix":"10.4103","volume":"14","author":[{"given":"Sameena","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Microbiology, Dr D Y Patil Medical College, Hospital and Research Center Pimpri Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajashri","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr D Y Patil Medical College, Hospital and Research Center Pimpri Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahzad","family":"Mirza","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr D Y Patil Medical College, Hospital and Research Center Pimpri Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nageswari","family":"Gandham","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr D Y Patil Medical College, Hospital and Research Center Pimpri Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parag","family":"Ratnakar","sequence":"additional","affiliation":[{"name":"Central Clinical Laboratory, Dr D Y Patil Medical College, Hospital and Research Center Pimpri, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanda","family":"Vyawahare","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr D Y Patil Medical College, Hospital and Research Center Pimpri Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niknja Kumar","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Microbiology, DRIEMS Institute of Health Sciences and Hospitals, Kotasahi, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"key":"R1-20251230","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/d41573-020-00073-5","article-title":"TheCOVID-19 vaccine development landscape","volume":"19","author":"Thanh","year":"2020","journal-title":"Nat Rev Drug Discov"},{"key":"R2-20251230","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.4103\/jfmpc.jfmpc_2486_20","article-title":"Seroprevalence of SARS-CoV-2 antibodies among healthcare workers in a teaching hospital in Eastern India","volume":"10","author":"Mishra","year":"2021","journal-title":"J Family Med Prim Care"},{"key":"R3-20251230","doi-asserted-by":"crossref","first-page":"270","DOI":"10.30699\/ijmm.17.2.270","article-title":"Are the current Covid-19 cases post phase of 3rd wave or pre-phase of 4th wave in India?","volume":"17","author":"Mukhida","year":"2023","journal-title":"Iran J Med Microbiol"},{"key":"R4-20251230","doi-asserted-by":"crossref","first-page":"9081491","DOI":"10.1155\/2021\/9081491","article-title":"High seroprevalence of SARS-CoV-2 (COVID-19)-specific antibodies among healthcare workers:A cross-sectional study in Guilan, Iran","volume":"2021","author":"Balou","year":"2021","journal-title":"J Environ Public Health"},{"key":"R5-20251230","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41368-020-0075-9","article-title":"Transmission routes of 2019-nCoV and controls in dental practice","volume":"12","author":"Peng","year":"2020","journal-title":"Int J Oral Sci"},{"key":"R6-20251230","doi-asserted-by":"crossref","first-page":"736","DOI":"10.3348\/kjr.2020.0171","article-title":"Association between initial chest CT or clinical features and clinical course in patients with coronavirus disease 2019 pneumonia","volume":"21","author":"Liu","year":"2020","journal-title":"Korean J Radiol"},{"key":"R7-20251230","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1177\/10105395221121049","article-title":"Are the diagnosed cases of tuberculosis just the tip of iceberg?Comments on \u201csyndemic of tuberculosis and COVID-19 in Bangladesh\u201d","volume":"34","author":"Mukhida","year":"2022","journal-title":"Asia Pac J Public Health"},{"key":"R8-20251230","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1093\/aje\/kwaa191","article-title":"COVID-19 in health-care workers:A living systematic review and meta-analysis of prevalence, risk factors, clinical characteristics, and outcomes","volume":"190","author":"G\u00f3mez-Ochoa","year":"2021","journal-title":"Am J Epidemiol"},{"key":"R9-20251230","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1001\/jamainternmed.2020.4214","article-title":"Prevalence of SARS-CoV-2 infection among health care workers in a tertiary community hospital","volume":"180","author":"Jeremias","year":"2020","journal-title":"JAMA Intern Med"},{"key":"R10-20251230","doi-asserted-by":"crossref","first-page":"960","DOI":"10.4103\/mjdrdypu.mjdrdypu_1004_22","article-title":"How long time to learn lessons and move on from pandemic?","volume":"15","author":"Mukhida","year":"2022","journal-title":"Med J DY Patil Vidyapeeth"},{"key":"R11-20251230","doi-asserted-by":"crossref","first-page":"3348","DOI":"10.18203\/2394-6040.ijcmph20212586","article-title":"Anti-SARS-CoV-2 IgG Seroprevalence among non-vaccinated healthcare workers:A study from rural north India","volume":"8","author":"Mane","year":"2021","journal-title":"Int J Community Med Public Health"},{"key":"R12-20251230","doi-asserted-by":"crossref","first-page":"e01136","DOI":"10.1002\/cti2.1136","article-title":"Detection of IgM and IgG antibodies in patients with coronavirus disease 2019","volume":"9","author":"Hou","year":"2020","journal-title":"Clin Transl Immunology"},{"key":"R13-20251230","doi-asserted-by":"crossref","first-page":"m1375","DOI":"10.1136\/bmj.m1375","article-title":"Covid-19:Four fifths of cases are asymptomatic, China figures indicate","volume":"369","author":"Day","year":"2020","journal-title":"BMJ"},{"key":"R14-20251230","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.jinf.2021.10.008","article-title":"SARS-CoV-2 vaccine breakthrough infection following a previous infection in a healthcare worker","volume":"84","author":"Gupta","year":"2022","journal-title":"J Infect"},{"key":"R15-20251230","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.4103\/jfmpc.jfmpc_780_23","article-title":"Seroprevalence of SARS-CoV-2 IgG in blood donors in a teaching institute from Western part of Maharashtra","volume":"13","author":"Patil","year":"2024","journal-title":"J Family Med Prim Care"},{"key":"R16-20251230","doi-asserted-by":"crossref","first-page":"418","DOI":"10.4103\/tcmj.tcmj_86_22","article-title":"Role of GeneXpert MTB\/RIF assay for the diagnosis of cervical lymph node tuberculosis and rifampicin resistance","volume":"34","author":"Mukhida","year":"2022","journal-title":"Tzu Chi Med J"},{"key":"R17-20251230","doi-asserted-by":"crossref","first-page":"8380","DOI":"10.1038\/s41598-021-87688-9","article-title":"SARS-CoV-2 seroprevalence in healthcare workers at a frontline hospital in Tokyo","volume":"11","author":"Fukuda","year":"2021","journal-title":"Sci Rep"},{"key":"R18-20251230","doi-asserted-by":"crossref","first-page":"104609","DOI":"10.1016\/j.jcv.2020.104609","article-title":"Comparative evaluation of SARS-CoV-2 IgG assays in India","volume":"131","author":"Chaudhuri","year":"2020","journal-title":"J Clin Virol"},{"key":"R19-20251230","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.ijid.2021.05.040","article-title":"SARS-CoV-2 seroprevalence among the general population and healthcare workers in India, December 2020-January 2021","volume":"108","author":"Murhekar","year":"2021","journal-title":"Int J Infect Dis"},{"key":"R20-20251230","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1186\/s12879-020-05662-8","article-title":"High prevalence of SARS-CoV-2 infection among symptomatic healthcare workers in a large university tertiary hospital in S\u00e3o Paulo, Brazil","volume":"20","author":"Buonafine","year":"2020","journal-title":"BMC Infect Dis"},{"key":"R21-20251230","doi-asserted-by":"crossref","first-page":"e051573","DOI":"10.1136\/bmjopen-2021-051573","article-title":"SARS-CoV-2 seroprevalence in healthcare workers of a teaching hospital in a highly endemic region in the Netherlands after the first wave:A cross-sectional study","volume":"11","author":"Bouwman","year":"2021","journal-title":"BMJ Open"},{"key":"R22-20251230","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1136\/thoraxjnl-2020-215414","article-title":"SARS-CoV-2 seroprevalence and asymptomatic viral carriage in healthcare workers:A cross-sectional study","volume":"75","author":"Shields","year":"2020","journal-title":"Thorax"},{"key":"R23-20251230","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.4103\/jfmpc.jfmpc_967_23","article-title":"A closer look at the link between cycle threshold, clinical features and biomarkers:An observational study in COVID-19 patients","volume":"13","author":"Kannuri","year":"2024","journal-title":"J Family Med Prim Care"}],"container-title":["Journal of Family Medicine and Primary Care"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jfmpc.jfmpc_136_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T18:59:32Z","timestamp":1786647572000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jfmpc.jfmpc_136_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/jfmpc.jfmpc_136_25","relation":{},"ISSN":["2249-4863","2278-7135"],"issn-type":[{"value":"2249-4863","type":"print"},{"value":"2278-7135","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-01-21","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-04","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}