{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:21:30Z","timestamp":1787840490177,"version":"build-2784847793"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>Intestinal parasitic infections (IPIs) are prevalent among children in developing countries, particularly in tropical and subtropical regions. These infections significantly impact physical and intellectual development and exacerbate nutritional deficiencies in early childhood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>This hospital-based cross-sectional study, conducted at AIIMS, Gorakhpur, from July to December 2023, involved 110 children aged six months to five years with diarrhea. The study compared the diagnostic performance of routine wet mount examination and stool concentration techniques (Formol-Ether Acetate Concentration [FAC] and Formal-Ether Concentration [FEC]) for identifying intestinal parasites.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result:<\/jats:title>\n                    <jats:p>FAC detected parasites in 75% of cases, FEC in 62%, and wet mount in 41%. Protozoan infections were predominant, with Blastocystis hominis, Entamoeba coli, Entamoeba histolytica, and Giardia lamblia being the most common. Dual infections were better detected by concentration methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The study highlighted the higher sensitivity of FAC over FEC and wet mount techniques. Environmental, socioeconomic, and geographical factors influenced the prevalence and distribution of IPIs. The study emphasizes the need for improved public health measures, including health education, safe water, and sanitation facilities. The formol\u2013ether acetate concentration technique is recommended for its higher recovery rate, safety, and feasibility in rural settings, requiring minimal infrastructure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jfmpc.jfmpc_1510_24","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T07:01:57Z","timestamp":1740034917000},"page":"693-698","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of different concentration techniques for microscopic diagnosis of protozoa and helminths in stool samples of children"],"prefix":"10.4103","volume":"14","author":[{"given":"Krati","family":"Agarwal","sequence":"first","affiliation":[{"name":"Department of Microbiology, AIIMS, Gorakhpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailja","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, Gorakhpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parul","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, Gorakhpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, Gorakhpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atul R","family":"Rukadikar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, Gorakhpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Hada","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, Gorakhpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aroop","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, Gorakhpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"R1-20260109","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1756-3305-7-37","article-title":"Global numbers of infection and disease burden of soil transmitted helminth infections in 2010","volume":"7","author":"Pullan","year":"2014","journal-title":"Parasit Vectors"},{"issue":"(Suppl 2)","key":"R2-20260109","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1093\/jn\/133.5.1661S","article-title":"Pregnancy, nutrition, and parasitic diseases","volume":"133","author":"Steketee","year":"2003","journal-title":"J Nutr"},{"key":"R3-20260109","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/S0140-6736(09)61798-0","article-title":"Diarrhoea:Why children are still dying and what can be done","volume":"375","author":"Wardlaw","year":"2010","journal-title":"Lancet"},{"key":"R4-20260109","doi-asserted-by":"crossref","first-page":"470","DOI":"10.4269\/ajtmh.1998.58.470","article-title":"Correlations between intestinal parasitosis, Physical growth, and psychomotor development among infants and children from rural Nicaragua","volume":"58","author":"Oberhelman","year":"1998","journal-title":"Am J Trop Med Hyg"},{"key":"R5-20260109","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/S0140-6736(02)07744-9","article-title":"Effects of stunting, diarrhoeal disease, and parasitic infection during infancy on cognition in late childhood:A follow-up study","volume":"359","author":"Berkman","year":"2002","journal-title":"Lancet"},{"key":"R6-20260109","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1046\/j.1365-3156.1998.00175.x","article-title":"Prevalence of intestinal parasites in rural southern Indians","volume":"3","author":"Kang","year":"1998","journal-title":"Trop Med Int Health"},{"key":"R7-20260109","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.jiph.2012.11.004","article-title":"The prevalence of intestinal parasitic infections in the urban slums of a city in Western India","volume":"6","author":"Shobha","year":"2013","journal-title":"J Infect Public Health"},{"key":"R8-20260109","doi-asserted-by":"crossref","first-page":"e0003768","DOI":"10.1371\/journal.pntd.0003768","article-title":"Effect of antihelminthic treatment on vaccine immunogenicity to a seasonal influenza vaccine in primary school children in Gabon:A randomized placebo- 35 controlled trial","volume":"9","author":"Br\u00fcckner","year":"2015","journal-title":"PLOS Negl Trop Dis"},{"key":"R9-20260109","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1007\/s12639-015-0645-5","article-title":"Low sensitivity of the ImmunocardSTAT\u00ae crypto\/giardia rapid assay test for the detection of giardia and cryptosporidium in fecal samples from children living in Libreville. 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