{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:23:15Z","timestamp":1787840595318,"version":"build-2784847793"},"reference-count":20,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Diagnosing childhood pulmonary tuberculosis (PTB) is challenging due to nonspecific symptoms and difficulties in obtaining respiratory specimens. This study evaluates the diagnostic performance of the stool-based Xpert MTB\/RIF assay as a noninvasive alternative compared to respiratory specimens in suspected cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods:<\/jats:title>\n                    <jats:p>This prospective, hospital-based study was carried out at a tertiary care centre, from November 2022 to October 2024. A total of 244 pediatric patients under 15 years of age with suspected PTB were included. Both stool and respiratory samples were collected and analyzed using the Xpert MTB\/RIF assay and Mycobacteria growth indicator tube (MGIT) culture. Data analysis was performed using IBM SPSS software (version 22).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The Xpert MTB\/RIF assay detected\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      in 10.2% of respiratory specimens and 6.1% of stool samples, while MGIT culture identified it in 8.6% of respiratory specimens and 5.3% of stool samples. Detection rates were higher in respiratory specimens. Stool-based diagnostics showed comparable performance, with reported sensitivities of 32% to 83.3% in prior studies. Baseline characteristics showed a mean patient age of 7.19 years, with 59.02% males. Fever (74.18%) and cough (52.8%) were the most common clinical features.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The stool Xpert MTB\/RIF assay is a promising diagnostic tool for PTB in children, especially in resource-limited settings where respiratory sample collection is difficult. Although its sensitivity is lower compared to respiratory specimens, its high specificity makes it a reliable alternative. Future research should optimize stool sample processing and explore advanced diagnostic assays to improve sensitivity and accuracy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jfmpc.jfmpc_2119_24","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:02:30Z","timestamp":1761742950000},"page":"4182-4186","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Stool Gene-Xpert MTB\/Rif test for the diagnosis of childhood pulmonary tuberculosis"],"prefix":"10.4103","volume":"14","author":[{"given":"Sweta","family":"Muni","sequence":"first","affiliation":[{"name":"Department of Microbiology, IGIMS, Patna, Bihar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, IGIMS, Patna, Bihar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Kishore","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, IGIMS, Patna, Bihar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namrata","family":"Kumari","sequence":"additional","affiliation":[{"name":"Department of Microbiology, IGIMS, Patna, Bihar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, IGIMS, Patna, Bihar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"key":"R2-20251105","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/S2213-2600(23)00463-0","article-title":"WHO paediatric tuberculosis roadmap","volume":"12","author":"Burki","year":"2024","journal-title":"Lancet Respir 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