{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T20:28:32Z","timestamp":1786739312911,"version":"build-2736575974"},"reference-count":10,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>\n                      A cohort study was carried out for a year to evaluate the presence of G-to-A transition in 5\u2032-untranslated region of ankylosis human (\n                      <jats:italic toggle=\"yes\">ANKH<\/jats:italic>\n                      ) gene in Indian Khatri patients (closely resembling Europeans of primary knee osteoarthritis (OA), residing in Lucknow, India.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      In the total participants, 25 were Khatri primary knee OA patients (cases) residing in Lucknow and 101 were random blood donors\u2019 samples (controls) collected from a blood bank. All were studied for the abovementioned mutation using real-time polymerase chain reaction (RT-PCR). GG genotype was present in 72.3% of controls and 76% of Khatri knee OA patients. The studied G-to-A mutation was found to be positive in 24.8% of controls and 16% of cases, odds ratio (95% confidence interval) being 0.6 (0.19\u20131.98,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.42). The frequency of AA (D) genotype found around 3% (cases) and 8% (controls) with\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      value of 0.70. The combined frequency of both homozygous and heterozygous mutation (GA and AA) in the studied population was 28 (27.7%) in controls and 6 (24%) in cases with the odds ratio (OD) ratio of 0.82 (0.29\u20132.27,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.70). No significant differences were observed at both genotype and allelic level in the distribution of\n                      <jats:italic toggle=\"yes\">ANKH-4<\/jats:italic>\n                      G-to-A gene polymorphism in studied subjects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>This study did not show any significant G to A mutation in the studied subjects.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jfmpc.jfmpc_471_19","type":"journal-article","created":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:49:11Z","timestamp":1569890951000},"page":"2937-2941","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Association of 4-basepair G-to-A transition in the 5\u2032-untranslated region of ANKH gene with selected patients of primary knee osteoarthritis"],"prefix":"10.4103","volume":"8","author":[{"given":"Puneet","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aarti","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddharth","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ragini","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-35-20260203","first-page":"570","article-title":"The high prevalence of pathologic calcium crystals in pre-operative knees","volume":"29","author":"Derfus","year":"2002","journal-title":"J Rheumatol"},{"key":"R2-35-20260203","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1086\/509881","article-title":"Biochemical and genetic analysis of ANK in arthritis and bone disease","volume":"79","author":"Gurley","year":"2006","journal-title":"Am J Hum Genet"},{"key":"R3-35-20260203","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.joca.2003.12.004","article-title":"Upregulated ANK expression in osteoarthritis can promote both chondrocyte MMP-13 expression and calcification via chondrocyte extracellular PPi excess","volume":"12","author":"Jhonson","year":"2004","journal-title":"Osteoarthritis Cartilage"},{"key":"R4-35-20260203","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1002\/art.20978","article-title":"Association of sporadic chondrocalcinosis with a -4-base pair G-to-A transition in the 5\u2019-untranslated region of ANKH that promotes Enhanced Expression of ANKH protein and excess generation of extracellular inorganic pyrophosphate","volume":"52","author":"Zhang","year":"2005","journal-title":"Arthritis Rheum"},{"key":"R5-35-20260203","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1002\/acr.21952","article-title":"Evidence of a systemic predisposition to chondrocalcinosis and association between chondrocalcinosis and osteoarthritis at distant joints: A cross-sectional study","volume":"65","author":"Abhishek","year":"2013","journal-title":"Arthritis Care Res"},{"key":"R6-35-20260203","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1086\/343054","article-title":"Mutations in ANKH cause chondrocalcinosis","volume":"71","author":"Pendleton","year":"2002","journal-title":"Am J Hum Genet"},{"key":"R7-35-20260203","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/nrrheum.2010.182","article-title":"Pathophysiology of articular chondrocalcinosis-role of ANKH","volume":"7","author":"Abhishek","year":"2011","journal-title":"Nat Rev Rheumatol"},{"key":"R8-35-20260203","first-page":"43","article-title":"Hereditary chondrocalcinosis in a Tunisian family","volume":"10","author":"Hamza","year":"1992","journal-title":"Clin Exp Rheumatol"},{"key":"R9-35-20260203","first-page":"109","article-title":"HLA antigens in a Tunisian familial chondrocalcinosis","volume":"8","author":"Hamza","year":"1990","journal-title":"Dis Markers"},{"key":"R10-35-20260203","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1136\/ard.34.3.260","article-title":"Familial chondrocalcinosis in the Chiloe islands, Chile","volume":"34","author":"Reginato","year":"1975","journal-title":"Ann Rheum Dis"}],"container-title":["Journal of Family Medicine and Primary Care"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jfmpc.jfmpc_471_19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T19:27:52Z","timestamp":1786735672000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jfmpc.jfmpc_471_19"}},"subtitle":["A cross sectional study"],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":10,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.4103\/jfmpc.jfmpc_471_19","relation":{},"ISSN":["2249-4863"],"issn-type":[{"value":"2249-4863","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]}}}