{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T17:26:05Z","timestamp":1793467565754,"version":"4.5.0"},"reference-count":10,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"<jats:p>Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis and hepatocellular carcinoma. Thus, this study was undertaken for detection and to observe genotypic distribution of the virus in this geographical region.<\/jats:p>\n                  <jats:sec>\n                    <jats:title>Aims and Objectives:<\/jats:title>\n                    <jats:p>The aim of the study was to quantitatively detect HCV RNA in Hepatitis C patients and to determine the distribution pattern of its genotypes by real time polymerase chain reaction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>This is a retrospective study conducted in a tertiary care hospital. In seropositive Hepatitis C patients, quantification of HCV-RNA was done by real time PCR and in HCV-RNA positive samples, genotyping for HCV was conducted.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result:<\/jats:title>\n                    <jats:p>A total of 300 patients were recruited in the study, of which 165 were anti HCV positive and among them, 127 were HCV-RNA positive. These positive samples were further subjected to genotype determination using real time PCR. 98 samples were positive for genotype, where genotype 3 was seen in 90 patients, genotype 1, in 3 patients and genotype 4, in 5 patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Knowledge of genotype is crucial for management of HCV infection and prediction of prognosis. Patients infected with HCV genotype 1 and 4 will have to receive IFN and ribavirin for a period of 48 weeks and show a poor sustained viral response. On the contrary, patients infected with HCV genotype 2 and 3 are reported to have better response to therapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jfmpc.jfmpc_651_20","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T06:48:16Z","timestamp":1604040496000},"page":"5249-5251","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Detection and distribution of genotypes of Hepatitis C in a tertiary care hospital"],"prefix":"10.4103","volume":"9","author":[{"given":"Upasana","family":"Bhumbla","sequence":"first","affiliation":[{"name":"Assistant Professor, Geetanjali Medical College and Hospital, Udaipur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laveena","family":"Shekhawat","sequence":"additional","affiliation":[{"name":"PG Student, Department of Microbiology, Geetanjali Medical College and Hospital, Udaipur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditi","family":"Kothari","sequence":"additional","affiliation":[{"name":"PG Student, Department of Microbiology, Geetanjali Medical College and Hospital, Udaipur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jatin","family":"Rao","sequence":"additional","affiliation":[{"name":"PG Student, Department of Microbiology, Geetanjali Medical College and Hospital, Udaipur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2020,10]]},"reference":[{"key":"R1-20260528","first-page":"9","article-title":"A major health problem of India","volume":"83","author":"Chattopadhyay","year":"2002","journal-title":"Curr Sci"},{"key":"R2-20260528","doi-asserted-by":"crossref","first-page":"349","DOI":"10.4103\/0255-0857.118875","article-title":"Genotypes of hepatitis C virus in the Indian sub-continent: A decade-long experience from a tertiary care hospital in South India","volume":"31","author":"Christdas","year":"2013","journal-title":"Indian J Med Microbiol"},{"key":"R3-20260528","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.1073\/pnas.88.6.2451","article-title":"Genetic organization and diversity of the hepatitis C virus","volume":"88","author":"Choo","year":"1991","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R4-20260528","doi-asserted-by":"crossref","first-page":"S21","DOI":"10.1002\/hep.1840360704","article-title":"Course and outcome of hepatitis C","volume":"36","author":"Hoofnagle","year":"2002","journal-title":"Hepatology"},{"key":"R5-20260528","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1056\/NEJMoa020047","article-title":"Peginterferon alpha-2a plus ribavirin for chronic hepatitis C virus infection","volume":"347","author":"Fried","year":"2002","journal-title":"N Engl J 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to interferon therapy","volume":"4","author":"Pang","year":"2009","journal-title":"PLoS One"},{"key":"R10-20260528","first-page":"983","article-title":"Prevelance of hepatitis C genotypes in Indian patients and their clinical significance","volume":"49","author":"Ammarapurkar","year":"2020","journal-title":"J Assoc Physicians India"}],"container-title":["Journal of Family Medicine and Primary Care"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jfmpc.jfmpc_651_20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T19:28:16Z","timestamp":1786735696000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jfmpc.jfmpc_651_20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":10,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.4103\/jfmpc.jfmpc_651_20","relation":{},"ISSN":["2249-4863","2278-7135"],"issn-type":[{"value":"2249-4863","type":"print"},{"value":"2278-7135","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"2020-04-18","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-30","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}