{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T19:06:07Z","timestamp":1793387167774,"version":"4.5.0"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,7]]},"abstract":"\n Background:<\/jats:title>\n Methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are multidrug-resistant bacteria that are difficult to treat because of their ability to form biofilms.<\/jats:p>\n <\/jats:sec>\n \n Objectives:<\/jats:title>\n In the present study, we evaluated the antibiotic-resistant phenotypes, biofilm-forming ability, and biofilm associated genes of 55 clinical MR-CoNS isolates obtained from two hospitals in Thailand.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n MALDI-TOF-MS and\n tuf<\/jats:italic>\n gene sequencing were performed to determine the species of all isolates. Biofilm production was determined using Congo red agar (CRA) and the microtiter plate (MTP) assay. Biofilm-associated genes were characterized using polymerase chain reaction (PCR).\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Among the 55 MR-CoNS isolates, five species were identified as\n Staphylococcus haemolyticus<\/jats:italic>\n (34.5%),\n Staphylococcus epidermidis<\/jats:italic>\n (32.7%),\n Staphylococcus capitis<\/jats:italic>\n (18.2%),\n Staphylococcus cohnii<\/jats:italic>\n (9.1%), and\n Staphylococcus hominis<\/jats:italic>\n (5.5%). The antimicrobial susceptibility pattern of MR-CoNS isolates indicated high resistance to cefoxitin (100%), penicillin (98.2%), erythromycin (96.4%), ciprofloxacin (67.3%), sulfamethoxazole\/trimethoprim (67.3%), gentamicin (67.3%), and clindamycin (63.6%). All the isolates were susceptible to vancomycin and linezolid. The biofilm production was detected in 87.3% isolates through the CRA method and in 38.1% isolates through the MTP assay. The prevalence rates of\n ica<\/jats:italic>\n AD,\n bap<\/jats:italic>\n ,\n fnb<\/jats:italic>\n A, and\n cna<\/jats:italic>\n were 18.2%, 12.7%, 47.3%, and 27.3%, respectively. There were significant differences in the presence of these biofilm-associated genes among the MR-CoNS isolates. Moreover, quantitative biofilm formation was significantly different among MR-CoNS species.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n The present study revealed that biofilm-associated genes are important for biofilm biomass in MR-CoNS isolates, and the findings of this study are essential for finding new strategies to control biofilm formation and prevent the spread of MR-CoNS infectious diseases.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jgid.jgid_118_18","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T07:56:31Z","timestamp":1566892591000},"page":"112-117","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":18,"title":["Biofilm Formation of Methicillin-resistant Coagulase-Negative Staphylococci Isolated from Clinical Samples in Northern Thailand"],"prefix":"10.4103","volume":"11","author":[{"given":"Thawatchai","family":"Kitti","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Oriental Medicine, Chiang Rai College, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rathanin","family":"Seng","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapee","family":"Thummeepak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Sciences, Naresuan University, Chiang Rai, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chalermchai","family":"Boonlao","sequence":"additional","affiliation":[{"name":"Department Clinical Microbiology, Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanyasiri","family":"Jindayok","sequence":"additional","affiliation":[{"name":"Division of Clinical Pathology, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sutthirat","family":"Sitthisak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Sciences, Naresuan University, Chiang Rai, Thailand"},{"name":"Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-20250523","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1128\/CMR.00109-13","article-title":"Coagulase-negative staphylococci","volume":"27","author":"Becker","year":"2014","journal-title":"Clin Microbiol 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