{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:37:49Z","timestamp":1786649869366,"version":"build-2736575974"},"reference-count":25,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10]]},"abstract":"\n A\n BSTRACT<\/jats:sc>\n <\/jats:title>\n \n Background:<\/jats:title>\n \n Genetic polymorphisms in the luteinizing hormone (LH) \u03b2-subunit gene have been associated with responses to controlled ovarian hyperstimulation (COH) in\n in vitro<\/jats:italic>\n fertilisation (IVF) patients. Variants in rs1800447 and rs34349826 may increase androgen production, potentially impairing folliculogenesis and ovarian response\n <\/jats:p>\n <\/jats:sec>\n \n Aim:<\/jats:title>\n The aim of this study was to investigate the clinical significance of LH \u03b2-subunit single-nucleotide polymorphism (SNP) (rs1800447 and rs34349826), levels of testosterone, sex hormone-binding globulin (SHBG) and free testosterone index in predicting COH response in polycystic ovary syndrome (PCOS) women<\/jats:p>\n <\/jats:sec>\n \n Settings and Design:<\/jats:title>\n This nested case\u2013control study enlisted 122 women with PCOS in the Morula IVF Jakarta Clinic, Jakarta, Indonesia<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n The selection of cases and controls was in the ratio of 1:2. Blood samples were taken on day 2 or 3 of menstrual cycle. Sanger sequencing was utilised to genotype the LH \u03b2-subunit genes (rs1800447 and rs34349826). Levels of testosterone and SHBG were measured to calculate the free testosterone index. Women were retrospectively grouped as hyporesponders (<8 oocytes) or normo\/hyperresponders (\u22658 oocytes) according to the number of retrieved oocytes<\/jats:p>\n <\/jats:sec>\n \n Statistical Analysis Used:<\/jats:title>\n \n SPSS Software version 21.0 (IBM Corp., USA). Independent\n t<\/jats:italic>\n -test or Mann\u2013Whitney test for numerical variables and Chi-square test for categorical variables were employed\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n A unique automatic transmission (AT) variant in both rs1800447 and rs34349826 LH \u03b2-subunit was discovered, which has yet been reported previously. Notably, the proportion of heterozygous LH \u03b2 genotypes (AT and AG) in both rs1800447 and rs34349826 was similar between hyporesponder and normo\/hyperresponder groups (\n P<\/jats:italic>\n > 0.05). All participants were normoandrogenic PCOS women, indicated by the normal level of testosterone (0.84 \u00b1 0.35 nmol\/L) and SHBG (57.87 \u00b1 29.20 nmol\/L) as well as free testosterone index (1.88 \u00b1 1.34). No difference in testosterone levels, SHBG and free testosterone index was observed among the studied groups (\n P<\/jats:italic>\n > 0.05)\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n LH \u03b2 gene SNPs (rs1800447 and rs34349826), testosterone level, SHBG level and free testosterone index were not significantly correlated and less effective clinical indicators for COH response in normoandrogenic PCOS women.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jhrs.jhrs_7_25","type":"journal-article","created":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T12:00:21Z","timestamp":1766836821000},"page":"239-245","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Luteinizing Hormone \u03b2-subunit Gene Polymorphisms and Androgen Levels are Less Predictive of Ovarian Response in Polycystic Ovary Syndrome In vitro Fertilisation Women: A Nested Case\u2013Control Study"],"prefix":"10.4103","volume":"18","author":[{"given":"Irham","family":"Suheimi","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia"},{"name":"Irsi Research and Training Centre, Jakarta, Indonesia"},{"name":"Morula IVF Jakarta Clinic, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Budi","family":"Wiweko","sequence":"additional","affiliation":[{"name":"Department Obstetric and Gynaecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalina","family":"Thuffi","sequence":"additional","affiliation":[{"name":"Morula IVF Jakarta Clinic, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raden","family":"Muharam","sequence":"additional","affiliation":[{"name":"Department Obstetric and Gynaecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Asmarinah","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia"},{"name":"Integrated Laboratory, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wresti","family":"Indriatmi","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safarina G.","family":"Malik","sequence":"additional","affiliation":[{"name":"Eijkman Institute for Molecular Biology, Jakarta, Indonesia"},{"name":"Mochtar Riady Institute for Nanotechnology, Banten, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arief","family":"Boediono","sequence":"additional","affiliation":[{"name":"Irsi Research and Training Centre, Jakarta, Indonesia"},{"name":"Morula IVF Jakarta Clinic, Jakarta, Indonesia"},{"name":"Department of Anatomy, Physiology and Pharmacology, IPB University, Bogor, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syarief Thaufik","family":"Hidayat","sequence":"additional","affiliation":[{"name":"Department Obstetric and Gynecology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Sini","sequence":"additional","affiliation":[{"name":"Irsi Research and Training Centre, Jakarta, Indonesia"},{"name":"Morula IVF Jakarta Clinic, Jakarta, Indonesia"},{"name":"Faculty of Medicine, IPB University, Bogor, West Java, 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