{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T19:18:03Z","timestamp":1792178283711,"version":"4.4.0"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10]]},"abstract":"\n Aim:<\/jats:title>\n \n This study aimed to evaluate the antimicrobial efficacy and cytocompatibility of methanolic\n Phoenix dactylifera<\/jats:italic>\n seed extract incorporated into three-dimensional (3D)-printed provisional dental resin against\n Porphyromonas gingivalis, Candida albicans<\/jats:italic>\n , and\n Staphylococcus<\/jats:italic>\n aureus<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Settings and Design:<\/jats:title>\n \n This study design involves an\n in vitro<\/jats:italic>\n experimental study.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Methanolic extract of\n P. dactylifera<\/jats:italic>\n seed was prepared and incorporated into a 3D-printed provisional dental resin at concentrations of 1.5%, 2.5%, and 5%. Antimicrobial activity was assessed by colony-forming unit (CFU\/ml) counts for each microorganism. Cytotoxicity was evaluated using the MTT assay on Vero cells after 48 h. Phytochemical screening was performed to identify the bioactive constituents in the extract.\n <\/jats:p>\n <\/jats:sec>\n \n Statistical Analysis Used:<\/jats:title>\n \n One-way Analysis of Variance followed by Tukey\u2019s\n post hoc<\/jats:italic>\n test was performed using IBM SPSS Statistics for Windows, Version 23.0.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Phytochemical analysis revealed reducing sugars, alkaloids, flavonoids, tannins, glycosides, proteins, and resins. Antimicrobial efficacy increased with concentration, with the 5% group showing the most significant reduction in CFU\/ml across all tested microorganisms (\n C. albicans<\/jats:italic>\n : 0.13;\n P. gingivalis<\/jats:italic>\n and\n S. aureus<\/jats:italic>\n :\n P<\/jats:italic>\n < 0.05). Cytotoxicity was minimal at 5% and 10% (cell viability >92%), while moderate cytotoxicity was observed at 25% (cell viability: 74%).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n Incorporation of\n P. dactylifera<\/jats:italic>\n seed extract into 3D-printed provisional dental resin enhanced antimicrobial activity against\n S. aureus, P. gingivalis<\/jats:italic>\n , and\n C. albicans<\/jats:italic>\n in a dose-dependent manner, with the 5% concentration showing the highest efficacy. Lower concentrations (1.5% and 2.5%) provided substantial antimicrobial effects while maintaining high biocompatibility, supporting their potential clinical use.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jips.jips_344_25","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:01:43Z","timestamp":1760936503000},"page":"369-376","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Antimicrobial efficacy of Phoenix dactylifera\u2013Infused three-dimensional-printed provisional dental resin against Porphyromonas gingivalis, Candida albicans, and Staphylococcus aureus: An in vitro study"],"prefix":"10.4103","volume":"25","author":[{"given":"Jeyaraj Brintha","family":"Jei","sequence":"first","affiliation":[{"name":"Department of Prosthodontics and Crown and Bridge, SRM Dental College, Ramapuram, Bharathi Salai, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muthukumar","family":"Balasubramaniam","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Crown and Bridge, SRM Dental College, Ramapuram, Bharathi Salai, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,10,20]]},"reference":[{"key":"R1-20260420","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.3390\/foods13071024","article-title":"An overview of date (Phoenix dactylifera) fruits as an important global food resource","volume":"13","author":"Al-Karmadi","year":"2024","journal-title":"Foods"},{"key":"R2-20260420","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4103\/JPBS.JPBS_168_18","article-title":"Date palm (Phoenix dactylifera L.) fruit as potential antioxidant and antimicrobial agents","volume":"11","author":"Al-Shwyeh","year":"2019","journal-title":"J Pharm Bioallied 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