{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:32:39Z","timestamp":1790289159109,"version":"4.1.0"},"reference-count":59,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract:<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Periodontitis is a multifactorial, polymicrobial oral inflammatory illness brought on by oral pathogens.\n                      <jats:italic toggle=\"yes\">Porphyromonas<\/jats:italic>\n                      <jats:italic toggle=\"yes\">gingivalis<\/jats:italic>\n                      is a Gram-negative, obligatory anaerobic, black-pigmented coccobacillus and is regarded as a primary etiological factor in the progression of periodontitis. Rapid, highly senstitive and specific detection methods are emerging. The present study aimed to evaluate the loop-mediated isothermal amplification (LAMP) technique for efficiently detecting\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">gingivalis<\/jats:italic>\n                      from subgingival plaque samples of chronic periodontitis patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      This study included 50 subgingival plaque samples from patients suffering from chronic periodontitis. The DNA (Deoxyribonucleic acid) was extracted by the \u201cmodified proteinase K\u201d method. A set of six primers, targeting the\n                      <jats:italic toggle=\"yes\">pepO<\/jats:italic>\n                      gene of\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">gingivalis<\/jats:italic>\n                      , was used for conducting LAMP. The amplification was visualized by naked-eye detection and agarose electrophoresis. Conventional polymerase chain reaction (PCR) and real-time qantitative PCR (qPCR) were carried out by targeting the 16SrRNA (16S ribosomal ribonucleic acid) gene of\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">gingivalis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The results showed that LAMP detected\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">gingivalis<\/jats:italic>\n                      in 40 out of 50 samples (80%). Whereas, qPCR and conventional PCR technique detected\n                      <jats:italic toggle=\"yes\">P. gingivalis<\/jats:italic>\n                      in 38 (76%) and 33 (66%) samples respectively. The sensitivity and specificity of the LAMP method were 94.87% and 90.90%, respectively. With qPCR, the sensitivity and specificity were found to be 92.30% and 81.81%, respectively, whereas, with conventional PCR, it was found to be 76.92% and 72.72%, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      LAMP is an efficient technique for quick, accurate, and reliable identification of\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">gingivalis<\/jats:italic>\n                      from subgingival plaque samples. The technique needs to be validated analytically, and further studies can be conducted by taking saliva and\/or gingival crevicular fluid samples from periodontitis patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jisp.jisp_260_23","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T17:52:37Z","timestamp":1717523557000},"page":"122-128","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteria Porphyromonas gingivalis"],"prefix":"10.4103","volume":"28","author":[{"given":"Meenaz N.","family":"Sangolli","sequence":"first","affiliation":[{"name":"Central Research Laboratory, Maratha Mandal\u2019s Nathajirao G. 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