{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:43:54Z","timestamp":1787071434077,"version":"build-2736575974"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract:<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Platelet-rich fibrin (PRF) is a widely used second-generation blood concentrate in periodontal regeneration. It has a few disadvantages, such as the fragile nature of the membrane, difficulty in suturing, and unpredictability of outcomes. The present study aims to evaluate the clinical outcomes, healing, and postoperative morbidity following the use of frozen advanced PRF (\u221220\u00b0C for 24 h) with modified coronally advanced tunnel (MCAT) technique in the treatment of multiple adjacent recession defects (MARDs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>This is a prospective, split-mouth, randomized controlled clinical trial including 42 teeth with bilateral MARD in 10 participants. Sites were randomized to frozen (Fz-PRF) and fresh advanced PRF (A-PRF) groups and treated with Fz-PRF using MCAT and A-PRF using MCAT, respectively. Root coverage parameters (recession depth [RD], recession width [RW], complete root coverage, percentage root coverage [%RC], mechanical characteristics [needle passage ease, surgical handling of the membrane, and membrane fraying], postoperative healing [Wachtel\u2019s Healing Index], and patient-reported outcomes [discomfort and esthetic satisfaction]) were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Mean %RC (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.025), RW (0.044), mechanical characteristics (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.000), and esthetic satisfaction (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.015) were significantly better in the Fz-PRF group. No significant variation was observed in patient discomfort and postoperative healing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Fz-PRF showed better root coverage, mechanical properties, and esthetic satisfaction than A-PRF; as a result, it might be an effective alternative to conventional methods of treating gingival recessions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jisp.jisp_419_24","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T08:02:28Z","timestamp":1765958548000},"page":"514-521","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Frozen advanced platelet-rich fibrin as an adjunct to the modified tunnel technique for root coverage: A pilot randomised clinical trial"],"prefix":"10.4103","volume":"29","author":[{"given":"Sruthima N. V. S.","family":"Gottumukkala","sequence":"first","affiliation":[{"name":"Department of Periodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murali","family":"Krishna","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gautami S.","family":"Penmetsa","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. S. V.","family":"Ramesh","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Mohan","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anusha","family":"Boddeda","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Implantology, GITAM Dental College, Visakhapatnam, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhavya","family":"Manchala","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"R1-20251220","doi-asserted-by":"crossref","first-page":"58","DOI":"10.3390\/bioengineering10010058","article-title":"Evolution and clinical advances of platelet-rich fibrin in musculoskeletal regeneration","volume":"10","author":"Narayanaswamy","year":"2023","journal-title":"Bioengineering (Basel)"},{"key":"R2-20251220","doi-asserted-by":"crossref","first-page":"S519","DOI":"10.4103\/jpbs.jpbs_491_25","article-title":"In vitro analysis of healing properties of platelet-rich fibrin (PRF) versus collagen membranes in guided tissue regeneration","volume":"17","author":"Kumar","year":"2025","journal-title":"J Pharm Bioallied Sci"},{"key":"R3-20251220","doi-asserted-by":"crossref","first-page":"e56","DOI":"10.1016\/j.tripleo.2005.07.011","article-title":"Platelet-rich fibrin (PRF):a second-generation platelet concentrate. 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periodontally healthy subjects","volume":"7","author":"Goel","year":"2021","journal-title":"Clin Exp Dent Res"},{"key":"R10-20251220","first-page":"72","article-title":"Effects of Choukroun's platelet-rich fibrin on human gingival fibroblasts proliferation, migration and type I collagen secretion","volume":"48","author":"Fan","year":"2013","journal-title":"Zhonghua Kou Qiang Yi Xue Za Zhi"},{"key":"R11-20251220","doi-asserted-by":"crossref","first-page":"3433","DOI":"10.3390\/ijms19113433","article-title":"Biological and mechanical properties of platelet-rich fibrin membranes after thermal manipulation and preparation in a single-syringe closed system","volume":"19","author":"Kardos","year":"2018","journal-title":"Int J Mol Sci"},{"key":"R12-20251220","first-page":"44903","article-title":"The impact of storage protocols on the mechanical and structural properties of frozen platelet-rich fibrin membranes","volume":"12","author":"Gottumukkala","year":"2025","journal-title":"Biomater 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