{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:20:53Z","timestamp":1787840453512,"version":"build-2784847793"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Periodontal disease is a chronic inflammatory condition that leads to the destruction of periodontal tissues, exacerbated by bacterial lipopolysaccharide (LPS). Current therapeutic approaches seek to reduce inflammation and promote regeneration. Curcumin, a natural polyphenol with anti-inflammatory and antioxidant properties, may offer a biocompatible alternative for periodontal regeneration by protecting human periodontal ligament cells (hPDLCs) from inflammatory damage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      The present\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      study examined the effects of curcumin on hPDLCs exposed to LPS (10 \u00b5g\/mL) to simulate an inflammatory environment. hPDLCs were divided into four groups: control, curcumin-treated (2.5 \u00b5M and 5 \u00b5M), LPS-induced inflammation, and LPS with curcumin. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and morphological changes were evaluated via phase-contrast microscopy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Curcumin treatment did not significantly impact cell viability in hPDLCs under noninflammatory conditions, with viability rates exceeding 94%. In LPS-stimulated cells, curcumin at 2.5 \u00b5M and 5 \u00b5M significantly improved viability from 60.1% (LPS only) to 80.1% and 87.6%, respectively. Morphological assessment revealed that curcumin mitigated LPS-induced cellular damage, promoting normal cell structure and growth.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Curcumin demonstrates potential as a therapeutic agent for periodontal regeneration by enhancing cell viability and counteracting LPS-induced cytotoxicity in hPDLCs. This study supports curcumin\u2019s role in creating a microenvironment conducive to periodontal tissue repair and highlights its promise for future applications in periodontal therapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jisp.jisp_461_24","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T11:00:44Z","timestamp":1755774044000},"page":"147-152","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the impact of curcumin concentrations on the viability of human periodontal ligament cells: An in vitro study"],"prefix":"10.4103","volume":"29","author":[{"given":"Merita","family":"Stanley","sequence":"first","affiliation":[{"name":"Department of Periodontology, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology, Chengalpattu, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Antony Prem Blaise","family":"Rajula","sequence":"additional","affiliation":[{"name":"Department of Periodontology, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology, Chengalpattu, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Potluri Leela Ravi","family":"Shankar","sequence":"additional","affiliation":[{"name":"Department of Periodontology, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology, Chengalpattu, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karkala Sunanda","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Periodontology, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology, Chengalpattu, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrutha","family":"Sairam","sequence":"additional","affiliation":[{"name":"Department of Periodontology, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology, Chengalpattu, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjana","family":"Jeyakumaran","sequence":"additional","affiliation":[{"name":"Department of Periodontology, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology, Chengalpattu, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,8,19]]},"reference":[{"key":"R1-20260421","doi-asserted-by":"crossref","first-page":"109","DOI":"10.4103\/jisp.jisp_173_19","article-title":"Comparative evaluation of the effect of curcumin and chlorhexidine on human fibroblast viability and migration: An in vitro study","volume":"24","author":"Sukumaran","year":"2020","journal-title":"J Indian Soc Periodontol"},{"key":"R2-20260421","doi-asserted-by":"crossref","first-page":"16593","DOI":"10.1038\/srep16593","article-title":"Quercitrin for periodontal regeneration: Effects on human gingival fibroblasts and mesenchymal stem cells","volume":"5","author":"G\u00f3mez-Florit","year":"2015","journal-title":"Sci Rep"},{"key":"R3-20260421","doi-asserted-by":"crossref","first-page":"139","DOI":"10.2174\/1874210601105010139","article-title":"In vitro osteogenic induction of human gingival fibroblasts for bone regeneration","volume":"5","author":"Mostafa","year":"2011","journal-title":"Open Dent J"},{"key":"R4-20260421","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1902\/annals.2003.8.1.227","article-title":"The efficacy of bone replacement grafts in the treatment of periodontal osseous defects. A systematic review","volume":"8","author":"Reynolds","year":"2003","journal-title":"Ann Periodontol"},{"key":"R5-20260421","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1002\/sctm.20-0161","article-title":"Cross talk between mesenchymal and glioblastoma stem cells: Communication beyond controversies","volume":"9","author":"Bajetto","year":"2020","journal-title":"Stem Cells Transl Med"},{"key":"R6-20260421","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0140-6736(04)16627-0","article-title":"Investigation of multipotent postnatal stem cells from human periodontal ligament","volume":"364","author":"Seo","year":"2004","journal-title":"Lancet"},{"key":"R7-20260421","first-page":"74","article-title":"Baricitinib alleviates lipopolysaccharideinduced human periodontal ligament stem cell injury and promotes osteogenic differentiation by inhibiting JAK\/STAT signaling","volume":"25","author":"Yang","year":"2023","journal-title":"Exp Ther Med"},{"key":"R8-20260421","doi-asserted-by":"crossref","first-page":"104927","DOI":"10.1016\/j.archoralbio.2020.104927","article-title":"Fraxinellone alleviates inflammation and promotes osteogenic differentiation in lipopolysaccharide-stimulated periodontal ligament stem cells by regulating the bone morphogenetic protein 2\/Smad pathway","volume":"121","author":"Fu","year":"2021","journal-title":"Arch Oral Biol"},{"key":"R9-20260421","doi-asserted-by":"crossref","first-page":"204","DOI":"10.4103\/jisp.jisp_396_23","article-title":"Comparative assessment of medicinal plant extract efficacy against plaque and gingivitis: A case-control study","volume":"28","author":"Agrawal","year":"2024","journal-title":"J Indian Soc Periodontol"},{"key":"R10-20260421","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.5958\/0976-5506.2019.03686.6","article-title":"Role of medicinal herbs in periodontal therapy","volume":"10","author":"Mohanty","year":"2019","journal-title":"Indian J Public Health Res Dev"},{"key":"R11-20260421","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1208\/s12248-012-9432-8","article-title":"Therapeutic roles of curcumin: Lessons learned from clinical trials","volume":"15","author":"Gupta","year":"2013","journal-title":"AAPS J"},{"key":"R12-20260421","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4103\/0976-9668.107253","article-title":"Role of curcumin in systemic and oral health: An overview","volume":"4","author":"Nagpal","year":"2013","journal-title":"J Nat Sci Biol Med"},{"key":"R13-20260421","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1007\/s11033-018-4309-2","article-title":"Uncoupling Warburg effect and stemness in CD133(+ve) cancer stem cells from Saos-2 (osteosarcoma) cell line under hypoxia","volume":"45","author":"Koka","year":"2018","journal-title":"Mol Biol Rep"},{"key":"R14-20260421","doi-asserted-by":"crossref","first-page":"107572","DOI":"10.1016\/j.clay.2024.107572","article-title":"Cytotoxicity and genotoxic impacts of LAPONITE\u00ae on murine adipose stem cells","volume":"261","author":"Cardoso","year":"2024","journal-title":"Appl Clay Sci"},{"issue":"Suppl 1","key":"R15-20260421","first-page":"S118","article-title":"Periodontal regeneration","volume":"54","author":"Ivanovski","year":"2009","journal-title":"Aust Dent J"},{"key":"R16-20260421","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.3390\/molecules26051332","article-title":"The antioxidant effect of curcumin and rutin on oxidative stress biomarkers in experimentally induced periodontitis in hyperglycemic wistar rats","volume":"26","author":"Iova","year":"2021","journal-title":"Molecules"},{"key":"R17-20260421","first-page":"350","article-title":"Role of CGF (Concentrated Growth Factor) in periodontal regeneration","volume":"9","author":"Nityasri","year":"2018","journal-title":"J Dent Health Oral Disord Ther"},{"key":"R18-20260421","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1034\/j.1600-0757.2000.2240113.x","article-title":"Tissue engineering: A new paradigm for periodontal regeneration based on molecular and cell biology","volume":"24","author":"Bartold","year":"2000","journal-title":"Periodontol 2000"},{"key":"R19-20260421","doi-asserted-by":"crossref","first-page":"2964","DOI":"10.3390\/polym14142964","article-title":"Locally applied repositioned hormones for oral bone and periodontal tissue engineering: A narrative review","volume":"14","author":"Abdel Nasser Atia","year":"2022","journal-title":"Polymers (Basel)"},{"key":"R20-20260421","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1601-5037.2009.00380.x","article-title":"Growth factors in periodontal regeneration","volume":"7","author":"Raja","year":"2009","journal-title":"Int J Dent Hyg"},{"key":"R21-20260421","first-page":"509","article-title":"Some biological properties of curcumin: A review","volume":"1","author":"Ali","year":"2006","journal-title":"Nat Prod Commun"},{"key":"R22-20260421","doi-asserted-by":"crossref","first-page":"273","DOI":"10.4103\/jisp.jisp_168_22","article-title":"Efficacy of cytotoxic effect of green tea catechins on the human periodontal fibroblasts and human dental pulp fibroblasts -An in vitro study","volume":"27","author":"Hattarki","year":"2023","journal-title":"J Indian Soc Periodontol"},{"key":"R23-20260421","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.1007\/s00784-018-2771-9","article-title":"Subgingival lipid A profile and endotoxin activity in periodontal health and disease","volume":"23","author":"Strachan","year":"2019","journal-title":"Clin Oral Investig"},{"key":"R24-20260421","doi-asserted-by":"crossref","first-page":"192","DOI":"10.4103\/ayu.AYU_294_18","article-title":"An in vitro evaluation of cytotoxicity of curcumin against human periodontal ligament fibroblasts","volume":"40","author":"Mandroli","year":"2019","journal-title":"Ayu"},{"key":"R25-20260421","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1159\/000161127","article-title":"Anti-inflammatory activity of curcumin in macrophages stimulated by lipopolysaccharides from Porphyromonas gingivalis","volume":"82","author":"Chen","year":"2008","journal-title":"Pharmacology"},{"key":"R26-20260421","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.3892\/ijo.2013.2050","article-title":"Curcumin-loaded nanoparticles induce apoptotic cell death through regulation of the function of MDR1 and reactive oxygen species in cisplatin-resistant CAR human oral cancer cells","volume":"43","author":"Chang","year":"2013","journal-title":"Int J Oncol"},{"key":"R27-20260421","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s13005-018-0169-1","article-title":"Protective effect and related mechanisms of curcumin in rat experimental periodontitis","volume":"14","author":"Xiao","year":"2018","journal-title":"Head Face Med"}],"container-title":["Journal of Indian Society of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jisp.jisp_461_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:23:09Z","timestamp":1787836989000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jisp.jisp_461_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/jisp.jisp_461_24","relation":{},"ISSN":["0972-124X","0975-1580"],"issn-type":[{"value":"0972-124X","type":"print"},{"value":"0975-1580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-11-15","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-20","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}