{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:34:15Z","timestamp":1787762055297,"version":"build-2784847793"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["JISPCD"],"published-print":{"date-parts":[[2023,5]]},"abstract":"\n A\n bstract<\/jats:sc>\n <\/jats:title>\n \n Aims and Objectives:<\/jats:title>\n \n The primary objective of this study is to determine the prevalence of Epstein\u2013Barr virus (EBV) in oral potentially malignant disorders (OPMDs) and oral cancer (OC) in a group of Thais using polymerase chain reaction (PCR) and Epstein\u2013Barr encoding regions (\n EBERs<\/jats:italic>\n )\n in situ<\/jats:italic>\n hybridization (ISH). The secondary objective is to investigate the risk factors of OC and the association between the presence of EBV and risk factors of OC\/site of oral lesions.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Sixty-one participants attending the screening project for OC and OPMDs at the Northeastern district hospitals of Thailand were recruited. Information related to risk factors and biopsy tissues for histopathological diagnosis was collected. Sixty-seven paraffin tissue blocks, including 52 OPMDs and 15 OC specimens, were investigated for EBV infection, using PCR analysis with latent membrane protein-1 (\n LMP-1<\/jats:italic>\n ) primer and\n EBERs<\/jats:italic>\n ISH. Pearson\u2019s Chi-square or Fisher\u2019s exact test was used to analyze the differences in variables between participants with OPMDs and OC, as appropriate. The association between EBV infection and related risk factors was analyzed using logistic regression with a significant level at 0.05.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Using PCR analysis, 8 of 67 specimens (11.94%) were positive for\n LMP-1.<\/jats:italic>\n Three cases of OPMDs were positive for both\n LMP-1<\/jats:italic>\n PCR and\n EBERs<\/jats:italic>\n ISH. Regarding risk factors of OC, the two most common risk factors were betel nut chewing (52.46%) and working in sunlight (42.62%). The habit of taking alcohol was significantly different between the OC and the OPMDs groups (\n p<\/jats:italic>\n = 0.009). The association between\n LMP-1<\/jats:italic>\n and the lesion at the tongue was statistically significant, with odds ratio = 4.900 (95% confidence interval = 1.046\u201322.943;\n p<\/jats:italic>\n = 0.044).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n The prevalence of EBV infection in this group of participants was low. However, OPMDs at the tongue exhibited a significant association with EBV infection.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jispcd.jispcd_235_22","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T10:04:13Z","timestamp":1688119453000},"page":"221-228","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Analysis of Epstein\u2013Barr virus infection in oral potentially malignant disorders and oral cancer: A cross-sectional study"],"prefix":"10.4103","volume":"13","author":[{"given":"Jintana","family":"Pankam","sequence":"first","affiliation":[{"name":"Development of Disease Management Model for Oral Cancer with an Integration Network of Screening, Surveillance, and Treatment from Primary Care Unit to Tertiary Care in Nakhon Ratchasima Province Project, Faculty of Dentistry, Mahidol University, Bangkok, 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