{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:43:33Z","timestamp":1786653813859,"version":"build-2736575974"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,1]]},"abstract":"\n Background:<\/jats:title>\n \n In cariology studies, mitis-salivarius-bacitracin (MSB) agar has been commonly considered as the selective medium for\n Streptococcus<\/jats:italic>\n mutans<\/jats:italic>\n growth. The present study was the part of a funded project (a noninferiority randomized controlled trial) which compared the efficacy of a plant extract-based mouth rinse with that of a fluoride mouth rinse on the\n S.<\/jats:italic>\n mutans<\/jats:italic>\n counts of the children.\n <\/jats:p>\n <\/jats:sec>\n \n Aim:<\/jats:title>\n \n This study aimed to identify the frequency of detection of\n S.<\/jats:italic>\n mutans<\/jats:italic>\n and nonstreptococcal bacterial species from the dental plaque of caries active children using a combined technique of anaerobic culture and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry.\n <\/jats:p>\n <\/jats:sec>\n \n Settings and Methods:<\/jats:title>\n Caries-active children (8\u201312 years old) were enrolled from a pediatric dental outpatient department at a tertiary care hospital. From each participant, dental plaque samples were collected from carious surfaces under sterilized conditions and then subjected to anaerobic culture. After 48 h of incubation, the bacterial colonies were isolated by sub-culture and identified by the MALDI-TOF.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n In all, 13 different bacterial species were isolated from the MSB agar medium. Other than\n S.<\/jats:italic>\n mutans<\/jats:italic>\n species, colonies of bacterial species such as\n Veillonella<\/jats:italic>\n dispar,<\/jats:italic>\n Streptococcus<\/jats:italic>\n anginosus<\/jats:italic>\n ,\n Veillonella<\/jats:italic>\n parvula<\/jats:italic>\n , and\n Streptococcus<\/jats:italic>\n gordonii<\/jats:italic>\n were also frequently observed from the medium\n .<\/jats:italic>\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n The study concluded that several bacterial strains, both streptococcal and nonstreptococcal, could be isolated from the MSB agar medium; hence, this medium should no longer be considered selective medium for the culture of\n S.<\/jats:italic>\n mutans<\/jats:italic>\n in clinical and epidemiological studies.\n <\/jats:p>\n 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