{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:22:47Z","timestamp":1787775767975,"version":"build-2784847793"},"reference-count":19,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background and Objectives:<\/jats:title>\n \n The purpose of this study was to evaluate the antimicrobial activity of methylene blue dye with 660-nm diode laser in cavity disinfection and to compare the total bacterial count in dentinal samples preexcavation, postexcavation, and postdisinfection. The study design was experimental\n in vivo<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n Fifteen children aged 5\u201312 years with 15 primary molars requiring atraumatic restorative treatment (ART) were selected. Dentinal samples were collected from the carious tooth with a sterile spoon excavator three times, i.e., before caries excavation (preexcavation), after hand excavation of caries (postexcavation), and after doing laser photodynamic therapy with methylene blue dye and 660-nm diode laser (postdisinfection) and sent for microbiological analysis. The samples were transported in reduced transport fluid. The total bacterial count was carried out by an experienced microbiologist immediately after reporting.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n A significant number of bacteria was present after hand excavation of caries using ART. The bacterial count significantly reduced postlaser disinfection. The total viable bacterial count was reduced to 87.17% with hand excavation of caries, whereas it was reduced to 98.59% postlaser disinfection using methylene blue dye with 660-nm diode laser.<\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n It was seen that ART cannot eliminate all the microorganisms. Methylene blue dye with a 660-nm diode laser was able to reduce the total bacterial count and can be effectively used as an antimicrobial cavity disinfectant.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jisppd.jisppd_258_24","type":"journal-article","created":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T13:00:12Z","timestamp":1736600412000},"page":"280-285","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Evaluating the efficacy of cavity disinfection using methylene blue dye with 660-nm diode laser on primary molars: An in vivo study"],"prefix":"10.4103","volume":"42","author":[{"given":"Mitaj Pandharinath","family":"Anvekar","sequence":"first","affiliation":[{"name":"Department of Pediatric and Preventive Dentistry, Maratha Mandal\u2019s Nathajirao G. 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