{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T19:18:56Z","timestamp":1759778336170},"reference-count":34,"publisher":"Scientific Scholar","issue":"02","license":[{"start":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T00:00:00Z","timestamp":1586131200000},"content-version":"vor","delay-in-days":371,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          OBJECTIVES: Gram-positive infections such as those by Staphylococcus aureus have contributed to the disease burden by increasing the morbidity and mortality rates in India. This study aims to determine the prevalence and the antibiotic susceptibility pattern of Gram-positive pathogens at a tertiary care hospital, Mumbai, Maharashtra, India.<\/jats:p><jats:p>\n          MATERIALS AND METHODS: This retrospective cross-sectional study was carried out from January, 2015 to December, 2017, at a tertiary care hospital in Mumbai, India. The clinical isolates were cultured, and identification was done using Vitek 2 culture system. The antibiotic susceptibility testing was done as per the Clinical Laboratory Standard Institute guidelines.<\/jats:p><jats:p>\n          RESULTS: Out of 2132 (29%) Gram-positive isolates, S. aureus (49%) was the most common encountered pathogen, followed by Enterococcus spp. (24.5%) and coagulase-negative Staphylococcus (16%). Majority of the S. aureus were observed in patients with skin and soft-tissue infections (61.2%) followed by those suffering from respiratory (41%) and bloodstream infections (35%). Among the infections caused by S. aureus, the prevalence of methicillin resistance was 30%. While the MRSA isolates showed lower sensitivity toward co-trimoxazole (39%), clindamycin (30%), erythromycin (23%), and ciprofloxacin (10%), they showed higher susceptibility to linezolid (98%), vancomycin (98%), and teicoplanin (98%). All the isolates were found to be sensitive to daptomycin and tigecycline. While vancomycin-resistant enterococci (VRE) formed 7.5%, the linezolid-resistant enterococcus species was as high as 4.1%.<\/jats:p><jats:p>\n          CONCLUSION: The study showed a high prevalence of MRSA and VRE, thereby emphasizing the increasing antimicrobial resistance pattern of the Gram-positive pathogens. Therefore, there is an urgent need for novel antimicrobial stewardship to restrict the ongoing resistance rate among the isolates.<\/jats:p>","DOI":"10.4103\/jlp.jlp_173_18","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T09:20:22Z","timestamp":1558603222000},"page":"144-148","source":"Crossref","is-referenced-by-count":18,"title":["Microbiological profile and antibiotic susceptibility pattern of Gram-positive isolates at a tertiary care hospital"],"prefix":"10.25259","volume":"11","author":[{"given":"Dhruv","family":"Mamtora","sequence":"additional","affiliation":[{"name":"Department of Microbiology, S. L. Raheja Hospital, Mumbai, Maharashtra, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjith","family":"Saseedharan","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, S. L. Raheja Hospital, Mumbai, Maharashtra, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pallavi","family":"Bhalekar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, S. L. Raheja Hospital, Mumbai, Maharashtra, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surekha","family":"Katakdhond","sequence":"additional","affiliation":[{"name":"Department of Microbiology, S. L. 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India: National centre for disease control, Directorate General of Health Services, Ministry of Health and family welfare, Government of India; 2016 (version 1). p. 2."},{"key":"key-10.4103\/0974-2727.258957-8","first-page":"53","volume-title":"Prevalence and genetic mechanisms of antimicrobial resistance in Staphylococcus species: A multicentre report of the Indian council of medical research antimicrobial resistance surveillance network","author":"Rajkumar","year":"2017","journal-title":"Indian J Med Microbiol","ISSN":"http:\/\/id.crossref.org\/issn\/0255-0857","issn-type":"print"},{"key":"key-10.4103\/0974-2727.258957-9","first-page":"1735","volume-title":"Prevalence of methicillin-resistant Staphylococcus aureus in various clinical samples in a tertiary-care hospital","author":"Trivedi","year":"2015","journal-title":"Int J Med Sci Public Health"},{"key":"key-10.4103\/0974-2727.258957-10","first-page":"371","volume-title":"Methicillin resistant Staphylococcus aureus (MRSA) in Malwa region of Punjab (North-west India)","author":"Jindal","year":"2016","journal-title":"Indian J Med Res","ISSN":"http:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-2727.258957-11","first-page":"207","volume-title":"Multidrug resistant Gram positive pathogens with special reference to MRSA and biofilm production in ICU patients: Recurrent challenge for clinicians","author":"Gupta","year":"2015","journal-title":"Int J Curr Microbiol App Sci"},{"key":"key-10.4103\/0974-2727.258957-12","first-page":"133","volume-title":"Surveillance of infection status of drug resistant Staphylococcus aureus in an Indian teaching hospital","author":"Dubey","year":"2013","journal-title":"Asian Pac J Trop Dis"},{"key":"key-10.4103\/0974-2727.258957-13","first-page":"111","volume-title":"Enterococcal infections & amp; antimicrobial resistance","author":"Sood","year":"2008","journal-title":"Indian J Med Res","ISSN":"http:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-2727.258957-14","first-page":"25","volume-title":"Antimicrobial resistance in Enterococcus faecalis at a tertiary care centre of Northern India","author":"Mathur","year":"2003","journal-title":"Indian J Med Res","ISSN":"http:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-2727.258957-15","first-page":"72","volume-title":"Significance of vancomycin resistant enterococci from urinary specimens at a tertiary care centre in Northern India","author":"Taneja","year":"2004","journal-title":"Indian J Med Res","ISSN":"http:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-2727.258957-16","first-page":"26","volume-title":"Emergence of VRE and their antimicrobial sensitivity pattern in a tertiary care teaching hospital","author":"Kaur","year":"2009","journal-title":"J Med Biol Sci"},{"key":"key-10.4103\/0974-2727.258957-17","first-page":"549","volume-title":"Phenotypic & genotypic characterization of vancomycin resistant Enterococcus isolates from clinical specimens","author":"Praharaj","year":"2013","journal-title":"Indian J Med Res","ISSN":"http:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-2727.258957-18","unstructured":"Clinical and Laboratory Standards Institute. 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