{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:50:07Z","timestamp":1722559807394},"reference-count":0,"publisher":"Scientific Scholar","issue":"04","license":[{"start":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T00:00:00Z","timestamp":1582156800000},"content-version":"vor","delay-in-days":507,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>\n          PURPOSE: Hemoglobin (Hb) F% is increased in up to half of beta-thalassemia (\u03b2-thal) carriers. Several polymorphisms have been linked to such variability in different populations, including HBG2 - 158(C&gt;T) (Xmn I polymorphism) on chromosome 11. To determine the role of this polymorphism in such variability among Iraqi Kurds, the current study was initiated.<\/jats:p><jats:p>\n          MATERIALS AND METHODS: A total of 102 consecutive patients diagnosed as \u03b2-thal minor were enrolled. The enrollees had their diagnosis based on peripheral blood counts and high-performance liquid chromatography to determine HbA2 and HbF. All enrollees had their DNA extracted by phenol-chloroform method and Xmn I polymorphism detected by restriction fragment length polymorphism-polymerase chain reaction.<\/jats:p><jats:p>\n          RESULTS: The mean age (standard deviation [SD]) of the 102 enrollees was 25.4 (14.0) years, and the enrollees included 48 males and 54 females. Xmn I polymorphism was identified in heterozygous state in 46 (45.1%) patients and in homozygous state in one patient (0.98%). Thus, the minor allele frequency of this polymorphism was 0.235 in the studied group. There were no significant differences in red cell indices and HbA2% in carriers of the minor allele compared to noncarriers, while HbF% and absolute HbF concentrations were significantly higher in the former subgroup (P = 0.032 and 0.014, respectively). This polymorphism's contribution to HbF variability was found to be 5.8% in the studied sample. Furthermore, those with HbF \u22652% were 3.2 folds more likely to carry the minor allele.<\/jats:p><jats:p>\n          CONCLUSIONS: Xmn I polymorphism is frequently encountered in Iraqi Kurds with \u03b2-thal minor, and it is significantly associated with higher fetal hemoglobin in these patients.<\/jats:p>","DOI":"10.4103\/jlp.jlp_22_18","type":"journal-article","created":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T19:23:16Z","timestamp":1582572196000},"page":"370-373","source":"Crossref","is-referenced-by-count":1,"title":["HBG2 -158 (C&gt;T) polymorphism and its contribution to fetal hemoglobin variability in Iraqi Kurds with beta-thalassemia minor"],"prefix":"10.25259","volume":"10","author":[{"given":"Dilan J.","family":"Albarawi","sequence":"additional","affiliation":[{"name":"Scientific Research Center, College of Science, University of Duhok, Duhok, Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amer A.","family":"Balatay","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Pharmacy, University of Duhok, Duhok, Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasir","family":"Al-Allawi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Scientific Research Center, College of Medicine, University of Duhok, Duhok, Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2020,2,20]]},"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/JLP.JLP_22_18.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T08:15:36Z","timestamp":1703405736000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/hbg2-158-ct-polymorphism-and-its-contribution-to-fetal-hemoglobin-variability-in-iraqi-kurds-with-beta-thalassemia-minor\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10]]},"references-count":0,"journal-issue":{"issue":"04","published-online":{"date-parts":[[2020,2,20]]},"published-print":{"date-parts":[[2018,10]]}},"URL":"https:\/\/doi.org\/10.4103\/jlp.jlp_22_18","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10]]}}}