{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T17:14:11Z","timestamp":1793898851530,"version":"4.8.0"},"reference-count":30,"publisher":"Scientific Scholar","issue":"04","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":189,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>PURPOSE: We aimed to determine the recent pattern of antibiotic resistance and assess the vancomycin susceptibility profile of clinical Staphylococcus aureus in view of emerging reports of vancomycin creep, reduced vancomycin susceptibility (RVS), including heterogeneous vancomycin-intermediate S. aureus (hVISA) and vancomycin-intermediate S. aureus, and vancomycin resistance in S. aureus isolates.<\/jats:p>\n                  <jats:p>MATERIALS AND METHODS: Consecutive, nonduplicate isolates of S. aureus between July 2015 and June 2016 were subjected to antimicrobial susceptibility testing using standard disk diffusion test or Etest as per the Clinical and Laboratory Standards Institute 2015. Detection of hVISA was done by glycopeptide resistance detection Etest according to the manufacturer's instructions in strains with vancomycin minimum inhibitory concentration of 1\u20132 \u03bcg\/ml.<\/jats:p>\n                  <jats:p>RESULTS: A total of 284 S. aureus were obtained from pus (175, 61.6%), respiratory tract (31, 10.9%), urine (27, 9.5%), blood (25, 8.8%), body fluids (18, 6.3%), and catheter tips (8, 2.8%).  127 (44.7%) isolates were methicillin resistant, and 158 (55.6%) were multidrug resistant. High resistance was observed to penicillin (81.7%), erythromycin (62.3%), and ciprofloxacin (52.1%), whereas the resistance was low to gentamicin (5.3%), rifampicin (8.1%), and doxycycline (9.5%). Two hundred and fifty-one (88.3%) isolates were fully susceptible to vancomycin, whereas 33 (11.6%) demonstrated RVS. All were uniformly susceptible to linezolid, tigecycline, and daptomycin.<\/jats:p>\n                  <jats:p>CONCLUSIONS: A moderately high percentage of S. aureus isolates demonstrated RVS, which may limit its usefulness in methicillin-resistant isolates and may be associated with increased complications in methicillin-susceptible infections. In view of increasing glycopeptide resistance, the susceptibility status of vancomycin along with other antibiotics among clinical S. aureus isolates should be investigated periodically.<\/jats:p>","DOI":"10.4103\/jlp.jlp_39_19","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:45:55Z","timestamp":1576716355000},"page":"340-345","source":"Crossref","is-referenced-by-count":20,"title":["Recent pattern of antibiotic resistance in Staphylococcus aureus clinical isolates in Eastern India and the emergence of reduced susceptibility to vancomycin"],"prefix":"10.25259","volume":"11","author":[{"given":"Srujana","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bijayini","family":"Behera","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhrajyoti","family":"Sahu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok Kumar","family":"Praharaj","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"key-10.4103\/0974-2727.273552-1","first-page":"S344","volume-title":"Epidemiology of methicillin-resistant Staphylococcus aureus","author":"Boucher","year":"2008","journal-title":"Clin Infect Dis"},{"key":"key-10.4103\/0974-2727.273552-2","first-page":"3","volume-title":", Paclibare PA, Cabrera EC, Rivera WL.Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolates from a tertiary hospital in the Philippines","author":"Valle","year":"2016","journal-title":"Trop Med Health"},{"key":"key-10.4103\/0974-2727.273552-3","first-page":"235","volume-title":"Prevalence and clinical impact of heterogeneous vancomycin-intermediate Staphylococcus aureus isolated from hospitalized patients","author":"Koh","year":"2016","journal-title":"Ann Lab Med"},{"key":"key-10.4103\/0974-2727.273552-4","first-page":"99","volume-title":"Reduced vancomycin susceptibility in Staphylococcus aureus, including vancomycin-intermediate and heterogeneous vancomycin-intermediate strains: Resistance mechanisms, laboratory detection, and clinical implications","author":"Howden","year":"2010","journal-title":"Clin Microbiol Rev"},{"key":"key-10.4103\/0974-2727.273552-5","first-page":"e73300","volume-title":"Reduced vancomycin susceptibility found in methicillin-resistant and methicillin-sensitive Staphylococcus aureus clinical isolates in Northeast China","author":"Hu","year":"2013","journal-title":"PLoS One"},{"key":"key-10.4103\/0974-2727.273552-6","first-page":"435","volume-title":"Molecular epidemiology and clinical characteristics of hetero-resistant vancomycin intermediate Staphylococcus aureus bacteremia in a Taiwan medical center","author":"Lin","year":"2012","journal-title":"J Microbiol Immunol Infect"},{"key":"key-10.4103\/0974-2727.273552-7","first-page":"1099","volume-title":"High vancomycin MIC and complicated methicillin-susceptible Staphylococcus aureus bacteremia","author":"Aguado","year":"2011","journal-title":"Emerg Infect Dis"},{"key":"key-10.4103\/0974-2727.273552-8","first-page":"1057","volume-title":"High MICs for vancomycin and daptomycin and complicated catheter-related bloodstream infections with methicillin-sensitive Staphylococcus aureus","author":"San-Juan","year":"2016","journal-title":"Emerg Infect Dis"},{"key":"key-10.4103\/0974-2727.273552-9","unstructured":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: 16th Informational Supplement. Clinical and Laboratory Standards Institute Document M100-S16. Wayne, PA: Clinical and Laboratory Standards Institute; 2006."},{"key":"key-10.4103\/0974-2727.273552-10","unstructured":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: 19th Informational Supplement. Clinical and Laboratory Standards Institute Document M100-S19. 19th ed.. 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Novel observations in 11 heteroresistant vancomycin-intermediate methicillin-resistant Staphylococcus aureus strains from South India. Genome Announc 2016;4. pii: e01425-16.","DOI":"10.1128\/genomeA.01425-16"},{"key":"key-10.4103\/0974-2727.273552-18","first-page":"94","volume-title":"In vitro activity of vancomycin and daptomycin against clinical isolates of Staphylococcus aureus and enterococci from India","author":"Jain","year":"2013","journal-title":"Int J Antimicrob Agents"},{"key":"key-10.4103\/0974-2727.273552-19","first-page":"545","volume-title":"Increasing trend of heterogeneous vancomycin intermediate Staphylococcus aureus in a tertiary care center of Northern India","author":"Singh","year":"2015","journal-title":"Microb Drug Resist"},{"key":"key-10.4103\/0974-2727.273552-20","unstructured":"Collee JG, Miles RS, Watt B. Tests for the identification of bacteria. In: Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Mackie and McCartney Practical Medical Microbiology. 14th ed. London: Churchill Livingstone; 1996. p. 131-45."},{"key":"key-10.4103\/0974-2727.273552-21","unstructured":"Clinical and Laboratory Standards Institute. Performance Standards for antimicrobial Susceptibility Testing: 25th Informational Supplement. Clinical and Laboratory Standards Institute Document M100-S25. 25th ed.. Wayne, PA: Clinical and Laboratory Standards Institute; 2015."},{"key":"key-10.4103\/0974-2727.273552-22","doi-asserted-by":"crossref","unstructured":"Holmes NE, Johnson PD, Howden BP. Relationship between vancomycin-resistant Staphylococcus aureus, vancomycin-intermediate S. aureus, high vancomycin MIC, and outcome in serious S. aureus infections. J Clin Microbiol 2012;50:2548-52.","DOI":"10.1128\/JCM.00775-12"},{"key":"key-10.4103\/0974-2727.273552-23","unstructured":"Pfizer Inc. (Wyeth Pharmaceuticals): Tygacil\u00ae Product Insert. Pfizer; 2013. Available from: http:\/\/www.pfizerpro.com\/hcp\/tygacil. [Last accessed on 2016 Jul 15]."},{"key":"key-10.4103\/0974-2727.273552-24","first-page":"268","volume-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","author":"Magiorakos","year":"2012","journal-title":"Clin Microbiol Infect"},{"key":"key-10.4103\/0974-2727.273552-25","first-page":"363","volume-title":"Methicillin resistant Staphylococcus aureus (MRSA) in India: Prevalence and susceptibility pattern","author":"Indian","year":"2013","journal-title":"Indian J Med Res","ISSN":"https:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-2727.273552-26","first-page":"49","volume-title":"Prevalence of methicillin resistant Staphylococcus aureus in a tertiary referral hospital in Eastern Uttar Pradesh","author":"Anupurba","year":"2003","journal-title":"Indian J Med Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0255-0857","issn-type":"print"},{"key":"key-10.4103\/0974-2727.273552-27","first-page":"57","volume-title":"High prevalence of multidrug-resistant MRSA in a tertiary care hospital of Northern India","author":"Tiwari","year":"2008","journal-title":"Infect Drug Resist"},{"key":"key-10.4103\/0974-2727.273552-28","first-page":"704","volume-title":"Vancomycin resistance among methicillin resistant Staphylococcus aureus isolates from intensive care units of tertiary care hospitals in Hyderabad","author":"Thati","year":"2011","journal-title":"Indian J Med Res","ISSN":"https:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-2727.273552-29","first-page":"728","volume-title":"Current pattern of antibiotic resistance in Staphylococcus aureus clinical isolates and the emergence of vancomycin resistance","author":"Taj","year":"2010","journal-title":"J Coll Physicians Surg Pak"},{"key":"key-10.4103\/0974-2727.273552-30","first-page":"1169","volume-title":"Development of reduced vancomycin susceptibility in methicillin-susceptible Staphylococcus aureus","author":"Pillai","year":"2009","journal-title":"Clin Infect Dis"}],"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/JLP.JLP_39_19.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T03:16:33Z","timestamp":1703387793000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/recent-pattern-of-antibiotic-resistance-in-staphylococcus-aureus-clinical-isolates-in-eastern-india-and-the-emergence-of-reduced-susceptibility-to-vancomycin\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":30,"journal-issue":{"issue":"04","published-online":{"date-parts":[[2020,4,7]]},"published-print":{"date-parts":[[2019,10]]}},"URL":"https:\/\/doi.org\/10.4103\/jlp.jlp_39_19","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10]]}}}