{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:32:47Z","timestamp":1789605167740,"version":"build-2803163510"},"reference-count":15,"publisher":"Scientific Scholar","issue":"01","license":[{"start":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T00:00:00Z","timestamp":1582070400000},"content-version":"vor","delay-in-days":779,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          CONTEXT: Hemoglobinopathies constitute the world's most common genetically inherited red blood cell disorder. Screening and accurate identification of hemoglobin (Hb) variants have become increasingly important in antenatal diagnosis and prevention of Hb disorders.<\/jats:p><jats:p>\n          AIM: The aim of this study was to screen and identify Hb fractions prevalent in the Central Reference Laboratory of India.<\/jats:p><jats:p>\n          MATERIALS AND METHODS: A total of 65,779 cases were screened for hemoglobinopathies on the bio-rad variant high-performance liquid chromatography (HPLC) system by beta-thalassemia short program. The retention times, proportion of the hemoglobin (%) and the peak characteristics for all hemoglobin fractions were recorded. Molecular analysis of the beta-globin gene was carried out by DNA sequencing on eight cases.<\/jats:p><jats:p>\n          RESULTS: Total number of abnormal Hb fractions on cation exchange-HPLC (CE-HPLC) was seen in 12,131 (18.44%) cases. Beta-thalassemia trait was the predominant genetic Hb disorder accounting for 7377 cases (11.21%) of the total cases. This was followed by sickle cell trait (2.01%), sickle cell disease (1.59%), beta-thalassemia syndrome (0.80%), HbE trait (0.79%), and borderline HbA2 (0.51%). Molecular characterization of eight rare cases of hemoglobin variants by beta-globin gene sequencing identified three cases of Hb Beth Israel, two cases of Hb Hofu trait, and one case each of Hb J Cambridge, Hb Mizunami, and Hb Sherwood Forest.<\/jats:p><jats:p>\n          CONCLUSION: Superior resolution, rapid assay time, and accurate quantification make CE-HPLC suitable for the routine investigation of hemoglobinopathies.<\/jats:p>","DOI":"10.4103\/jlp.jlp_57_17","type":"journal-article","created":{"date-parts":[[2018,1,3]],"date-time":"2018-01-03T08:43:08Z","timestamp":1514968988000},"page":"073-079","source":"Crossref","is-referenced-by-count":44,"title":["Prevalence of hemoglobin variants and hemoglobinopathies using cation-exchange high-performance liquid chromatography in central reference laboratory of India: A report of 65779 cases"],"prefix":"10.25259","volume":"10","author":[{"given":"Sandeep","family":"Warghade","sequence":"additional","affiliation":[{"name":"Department of Hematology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyothi","family":"Britto","sequence":"additional","affiliation":[{"name":"Department of Hematology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reshma","family":"Haryan","sequence":"additional","affiliation":[{"name":"Department of Hematology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tejaswi","family":"Dalvi","sequence":"additional","affiliation":[{"name":"Department of Hematology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Bendre","sequence":"additional","affiliation":[{"name":"Department of Hematology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratiksha","family":"Chheda","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunmeet","family":"Matkar","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yogita","family":"Salunkhe","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milind","family":"Chanekar","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilesh","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology, Metropolis Healthcare Ltd, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2020,2,19]]},"reference":[{"key":"key-10.4103\/0974-2727.222163-1","first-page":"2","volume-title":"The genetic burden of hemoglobinopathies with special reference to community health in India and the challenges ahead","author":"Balgir","year":"2002","journal-title":"Indian J Hematol Blood Transfus"},{"key":"key-10.4103\/0974-2727.222163-2","first-page":"112","volume-title":"Prevalence of common hemoglobinopathies in Gujarat: An analysis of a large population screening programme","author":"Patel","year":"2012","journal-title":"Natl J Community Med"},{"key":"key-10.4103\/0974-2727.222163-3","first-page":"105","volume-title":"Prevalence of thalassemia and hemoglobinopathy in eastern India: A 10-year high-performance liquid chromatography study of 119,336 cases","author":"Mondal","year":"2016","journal-title":"Asian J Transfus Sci","ISSN":"https:\/\/id.crossref.org\/issn\/0973-6247","issn-type":"print"},{"key":"key-10.4103\/0974-2727.222163-4","first-page":"397","volume-title":"Amulti-center study in order to further define the molecular basis of beta-thalassemia in Thailand, Pakistan, Sri Lanka, Mauritius, Syria, and India, and to develop a simple molecular diagnostic strategy by amplification refractory mutation system-polymerase chain reaction","author":"Old","year":"2001","journal-title":"Hemoglobin"},{"key":"key-10.4103\/0974-2727.222163-5","first-page":"163","volume-title":"Molecular genetic testing of beta-thalassemia patients of Indian origin and a novel 8-bp deletion mutation at codons 36\/37\/38\/39","author":"Gupta","year":"2003","journal-title":"Genet Test"},{"key":"key-10.4103\/0974-2727.222163-6","unstructured":"Christianson A, Howson CP, Modell B. March Dimes Global of Report on Birth Defects: The Hidden Toll of Dying and Disabled Children. March of Dimes Birth Defects Foundation; 2006."},{"key":"key-10.4103\/0974-2727.222163-7","first-page":"309","volume-title":"Advantages in the use of high performance liquid chromatography technique for screening hemoglobinopathies in Venezuela","author":"Bravo-Urquiola","year":"2004","journal-title":"Invest Clin"},{"key":"key-10.4103\/0974-2727.222163-8","first-page":"57","volume-title":"Detection of Hb variants and hemoglobinopathies in Indian population using HPLC: Report of 2600 cases","author":"Sachdev","year":"2010","journal-title":"Indian J Pathol Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0377-4929","issn-type":"print"},{"key":"key-10.4103\/0974-2727.222163-9","first-page":"35","volume-title":"Study of hemoglobinopathies in patients of anemia using high performance liquid chromatography (HPLC) in Western India","author":"Bhalodia","year":"2015","journal-title":"Natl J Community Med"},{"key":"key-10.4103\/0974-2727.222163-10","first-page":"1380","volume-title":"Significance of borderline hemoglobin A2 values in an Italian population with a high prevalence of beta-thalassemia","author":"Giambona","year":"2008","journal-title":"Haematologica"},{"key":"key-10.4103\/0974-2727.222163-11","first-page":"275","volume-title":"Discrimination of beta-thalassemia minor and iron deficiency anemia by screening test for blood cell indices","author":"Aziz","year":"2012","journal-title":"Turk J Med Sci"},{"key":"key-10.4103\/0974-2727.222163-12","first-page":"1536","volume-title":"The burden of hemoglobinopathies in India and the challenges ahead","author":"Balgir","year":"2000","journal-title":"Curr Sci"},{"key":"key-10.4103\/0974-2727.222163-13","first-page":"343","volume-title":"Hemoglobin E disease in North Indian population: A report of 11 cases","author":"Kishore","year":"2007","journal-title":"Hematology"},{"key":"key-10.4103\/0974-2727.222163-14","first-page":"1284","volume-title":"Laboratory investigation of hemoglobinopathies and thalassemias: Review and update","author":"Clarke","year":"2000","journal-title":"Clin Chem"},{"key":"key-10.4103\/0974-2727.222163-15","first-page":"109","volume-title":"Microcytic hypochromic anemia: Should high performance liquid chromatography be used routinely for screening anemic and antenatal patients?","author":"Philip","year":"2013","journal-title":"Indian J Pathol Microbiol","ISSN":"https:\/\/id.crossref.org\/issn\/0377-4929","issn-type":"print"}],"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/JLP.JLP_57_17.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T08:15:06Z","timestamp":1703405706000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/prevalence-of-hemoglobin-variants-and-hemoglobinopathies-using-cation-exchange-high-performance-liquid-chromatography-in-central-reference-laboratory-of-india-a-report-of-65779-cases\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":15,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2020,2,19]]},"published-print":{"date-parts":[[2018,1]]}},"URL":"https:\/\/doi.org\/10.4103\/jlp.jlp_57_17","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1]]}}}