{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T19:50:28Z","timestamp":1722541828150},"reference-count":30,"publisher":"Scientific Scholar","issue":"04","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":189,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Department of Science and Technology under INSPIRE-fellowship scheme","award":["reference number IF150368"],"award-info":[{"award-number":["reference number IF150368"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Lab Physicians"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          BACKGROUND: The carbapenemase-encoding genes, bla\n          \n            NDM-\n           and bla\n          \n            OXA-48\n          \n          \n            -\n          \n          \n            like\n          , confer resistance to all the known beta-lactams and are encountered along with other beta-lactamase-encoding genes and\/or 16S ribosomal RNA (rRNA)-methylating genes. The co-occurrence of bla\n          \n            NDM\n           and bla\n          \n            OXA-48\n          \n          \n            -\n          \n          \n            like\n           on a single plasmid is a rare occurrence.<\/jats:p><jats:p>\n          AIM AND OBJECTIVE: The purpose of the study was to characterize the plasmids in Klebsiella pneumoniae isolates producing 16S rRNA methyltransferase along with bla\n          \n            NDM\n          , bla\n          \n            OXA-48-like\n          , and other resistance encoding genes.<\/jats:p><jats:p>\n          MATERIALS AND METHODS: One-hundred and seventeen K. pneumoniae clinical isolates which were resistant to aminoglycosides were collected. Polymerase chain reaction-based screening for 16S rRNA methyltransferase genes armA, rmtB, and rmtC; carbapenamase genes bla\n          \n            NDM\n          , bla\n          \n            OXA-48-like\n          , bla\n          \n            IMP,\n          \n          bla\n          \n            VIM,\n          and bla\n          \n            KPC\n          ; and other resistance genes such as bla\n          \n            TEM,\n          \n          bla\n          \n            SHV,\n          \n          bla\n          \n            CTX-M\n          , and qnr (A, B, and S) determinants acc (6') Ib-cr was performed. Conjugation experiment was carried out for seven isolates that anchored bla\n          \n            NDM\n          and bla\n          \n            OXA-48-like\n          along with any one of the 16S rRNA methyltransferases. The plasmid-based replicon typing for different plasmid-incompatible (Inc) group was performed on the conjugatively transferable plasmids.<\/jats:p><jats:p>\n          RESULTS: Among the 16S rRNA methyltransferases, armA was more predominant. bla\n          \n            NDM\n          and bla\n          \n            OXA-48\n          \n          \n            -like\n          were present in 56 (47.86%) and 22 (18.80%) isolates, respectively. Out of seven isolates which were conjugatively transferable, only four had bla\n          \n            NDM\n          and bla\n          \n            OXA-48\n          \n          \n            -\n          \n          \n            like\n          on the same plasmid and they belonged to Inc N and A\/C replicon. Three isolates co-harbored 16S rRNA methyltransferases armA, rmtB, and rmtC, and out of the them, one isolate harbored two 16S rRNA methyltransferases armA and rmtB, on the single-plasmid replicon A\/C.<\/jats:p><jats:p>\n          CONCLUSION: This is the  first report revealing the coexistence of bla\n          \n            NDM\n          and bla\n          \n            OXA-48\n          \n          \n            -\n          \n          \n            like\n          co-harboring two 16S rRNA methylases on a single conjugative plasmid replicon belonging to incompatibility group A\/C.<\/jats:p>","DOI":"10.4103\/jlp.jlp_59_19","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T05:45:55Z","timestamp":1576734355000},"page":"305-311","source":"Crossref","is-referenced-by-count":7,"title":["Co occurrence of two 16S rRNA methyltrasferases along with NDM and OXA 48 like carbapenamases on a single plasmid in Klebsiella pneumoniae"],"prefix":"10.25259","volume":"11","author":[{"given":"K. V.","family":"L. Aishwarya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. V.","family":"Geetha","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Shanthi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Uma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sri Ramachandra Institute for Higher Education and Research, Chennai, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"key-10.4103\/0974-2727.273557-1","first-page":"1888","volume-title":"Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa","author":"Yokoyama","year":"2003","journal-title":"Lancet"},{"key":"key-10.4103\/0974-2727.273557-2","first-page":"2565","volume-title":"Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation","author":"Galimand","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"key-10.4103\/0974-2727.273557-3","first-page":"2059","volume-title":"RmtC and rmtF 16S rRNA methyltransferase in NDM-1-producing Pseudomonas aeruginosa","author":"Rahman","year":"2015","journal-title":"Emerg Infect Dis"},{"key":"key-10.4103\/0974-2727.273557-4","first-page":"1409","volume-title":"Occurrence of armA and rmtB aminoglycoside resistance 16S rRNA methylases in extended-spectrum \u03b2-lactamases producing Escherichia coli in Algerian hospitals","author":"Ayad","year":"2016","journal-title":"Front Microbiol"},{"key":"key-10.4103\/0974-2727.273557-5","first-page":"e01037","volume-title":"Occurrence of acquired 16S rRNA methyltransferase-mediated aminoglycoside resistance in clinical isolates of Enterobacteriaceae within a tertiary referral hospital of Northeast India","author":"Wangkheimayum","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"key-10.4103\/0974-2727.273557-6","first-page":"442","volume-title":"Updated multiplex polymerase chain reaction for detection of 16S rRNA methylases: High prevalence among NDM-1 producers","author":"Ber\u00e7ot","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"key":"key-10.4103\/0974-2727.273557-7","first-page":"604","volume-title":"Characterization of Acinetobacter baumannii clinical isolates carrying bla (OXA-23) carbapenemase and 16S rRNA methylase ArmA genes in Yemen","author":"Bakour","year":"2014","journal-title":"Microb Drug Resist"},{"key":"key-10.4103\/0974-2727.273557-8","first-page":"515","volume-title":"Emergence of OXA-carbapenemase \u2013 And 16S rRNA methylase-producing international clones of Acinetobacter baumannii in Norway","author":"Karah","year":"2011","journal-title":"J Med Microbiol"},{"key":"key-10.4103\/0974-2727.273557-9","first-page":"E421","volume-title":"Emergence of Klebsiella pneumoniae co-producing NDM-type and OXA-181 carbapenemases","author":"Balm","year":"2013","journal-title":"Clin Microbiol Infect"},{"key":"key-10.4103\/0974-2727.273557-10","first-page":"e33990","volume-title":"NDM-1 and rmtC-producing Klebsiella pneumoniae isolates in Turkey","author":"Gokmen","year":"2016","journal-title":"Jundishapur J Microbiol"},{"key":"key-10.4103\/0974-2727.273557-11","first-page":"5403","volume-title":"Genetic features of blaNDM-1-positive Enterobacteriaceae","author":"Poirel","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"key-10.4103\/0974-2727.273557-12","first-page":"219","volume-title":"Identification of plasmids by PCR-based replicon typing","author":"Carattoli","year":"2005","journal-title":"J Microbiol Methods"},{"key":"key-10.4103\/0974-2727.273557-13","unstructured":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. CLSI Supplement M100S. CLSI Supplement M100S. Wayne, PA: Clinical and Laboratory Standards Institute; 2016."},{"key":"key-10.4103\/0974-2727.273557-14","first-page":"1007","volume-title":"Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals","author":"Yan","year":"2004","journal-title":"J Antimicrob Chemother"},{"key":"key-10.4103\/0974-2727.273557-15","first-page":"88","volume-title":"16S ribosomal RNA methylation: Emerging resistance mechanism against aminoglycosides","author":"Doi","year":"2007","journal-title":"Clin Infect Dis"},{"key":"key-10.4103\/0974-2727.273557-16","first-page":"1791","volume-title":"Global spread of carbapenemase-producing Enterobacteriaceae","author":"Nordmann","year":"2011","journal-title":"Emerg Infect Dis"},{"key":"key-10.4103\/0974-2727.273557-17","first-page":"618","volume-title":"Detection of extended spectrum beta-lactamases-producing isolates and effect of AmpC overlapping","author":"Hassan","year":"2013","journal-title":"J Infect Dev Ctries"},{"key":"key-10.4103\/0974-2727.273557-18","first-page":"2872","volume-title":"Qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States","author":"Robicsek","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"key-10.4103\/0974-2727.273557-19","first-page":"476","volume-title":"Allele-specific polymerase chain reaction (PCR) for rapid detection of the aac (6')-Ib-cr quinolone resistance gene","author":"Wareham","year":"2010","journal-title":"Int J Antimicrob Agents"},{"key":"key-10.4103\/0974-2727.273557-20","first-page":"111","volume-title":"Plasmid-mediated transfer of the bla (NDM-1) gene in gram-negative rods","author":"Potron","year":"2011","journal-title":"FEMS Microbiol Lett"},{"key":"key-10.4103\/0974-2727.273557-21","first-page":"227","volume-title":"Antimicrobial resistance among clinical isolates from the Chinese meropenem surveillance study (CMSS), 2003-2008","author":"Wang","year":"2010","journal-title":"Int J Antimicrob Agents"},{"key":"key-10.4103\/0974-2727.273557-22","first-page":"133","volume-title":"Exogenously acquired 16S rRNA methylases found in aminoglycoside- resistant pathogenic gram negative bacteria: An update","author":"Wachino","year":"2012","journal-title":"Drug Resist Updat"},{"key":"key-10.4103\/0974-2727.273557-23","first-page":"348","volume-title":"Diverse prevalence of 16S rRNA methylase genes armA and rmtB amongst clinical multidrug-resistant Escherichia coli and Klebsiella pneumoniae isolates","author":"Yang","year":"2011","journal-title":"Int J Antimicrob Agents"},{"key":"key-10.4103\/0974-2727.273557-24","first-page":"161","volume-title":"Correlation of TEM, SHV and CTX-M extended-spectrum beta lactamases among Enterobacteriaceae with theirin vitro antimicrobial susceptibility","author":"Manoharan","year":"2011","journal-title":"Indian J Med Microbiol","ISSN":"http:\/\/id.crossref.org\/issn\/0255-0857","issn-type":"print"},{"key":"key-10.4103\/0974-2727.273557-25","first-page":"695","volume-title":"Extended spectrum beta-lactamases in Escherichia coli and Klebsiella pneumoniae and associated risk factors","author":"Goyal","year":"2009","journal-title":"Indian J Med Res","ISSN":"http:\/\/id.crossref.org\/issn\/0971-5916","issn-type":"print"},{"key":"key-10.4103\/0974-2727.273557-26","first-page":"240","volume-title":"Carbapenem resistant Klebsiella pneumoniae isolated from bloodstream infection: Indian experience","author":"Veeraraghavan","year":"2017","journal-title":"Pathog Glob Health"},{"key":"key-10.4103\/0974-2727.273557-27","first-page":"2949","volume-title":"Worldwide disseminated armA aminoglycoside resistance methylase gene is borne by composite transposon Tn1548","author":"Galimand","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"key-10.4103\/0974-2727.273557-28","first-page":"142","volume-title":"Occurrence of OXA-48 and NDM-1 carbapenemase-producing Klebsiella pneumoniae in a Moroccan University hospital in Casablanca, Morocco","author":"Barguigua","year":"2015","journal-title":"Infect Genet Evol"},{"key":"key-10.4103\/0974-2727.273557-29","unstructured":"Soundari PG, Vijyakumar R, Krishnan P. Abstracts of the 27th European Congress of Clinical Microbiology and Infectious Diseases. Vienna, Austria; 2017."},{"key":"key-10.4103\/0974-2727.273557-30","first-page":"4914","volume-title":"Emergence of metallo-\u03b2-lactamase NDM-1-producing multidrug-resistant Escherichia coli in Australia","author":"Poirel","year":"2010","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Journal of Laboratory Physicians"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.4103\/JLP.JLP_59_19.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T08:16:29Z","timestamp":1703405789000},"score":1,"resource":{"primary":{"URL":"https:\/\/jlabphy.org\/co-occurrence-of-two-16s-rrna-methyltrasferases-along-with-ndm-and-oxa-48-like-carbapenamases-on-a-single-plasmid-in-klebsiella-pneumoniae\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":30,"journal-issue":{"issue":"04","published-online":{"date-parts":[[2020,4,7]]},"published-print":{"date-parts":[[2019,10]]}},"URL":"https:\/\/doi.org\/10.4103\/jlp.jlp_59_19","relation":{},"ISSN":["0974-2727","0974-7826"],"issn-type":[{"value":"0974-2727","type":"print"},{"value":"0974-7826","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10]]}}}