{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T16:19:11Z","timestamp":1787847551464,"version":"build-2784847793"},"reference-count":14,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Chronic tonsillitis (CT) is a common inflammatory illness in children, and serum antistreptolysin O titer (ASOT) is a common investigation performed for these cases and considered a perfect sign for tonsillectomy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To evaluate the expression of tonsillar T-and B-lymphocytes markers in relation to seropositive or seronegative ASOT in cases of CT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>Thirty children (15 males and 15 females) aged 6\u201310 years were divided equally into two groups: Group A seropositive ASOT (\u2265400 IU) and Group B seronegative ASOT (&lt;400 IU). Both performed bilateral tonsillectomy. Specimens from the removed tonsils were taken and prepared for light microscopic examination and immunohistochemical evaluation of CD20 and CD3 expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Seropositive ASOT group showed significant histopathologic changes in the form of hyperplasia of the stratified squamous nonkeratinized epithelium, Urgas's abscess, and severe lymphocytic infiltration. Immunohistochemical results of seropositive ASOT group showed marked expression of CD3 and CD20, while seronegative ASOT group showed mild expression of CD3 and CD20.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Seropositive ASOT CT, in addition to histopathological changes, is associated with significant increase in both B-lymphocytes (CD20 expression) and T-lymphocytes (CD3 expression) markers.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jmau.jmau_80_20","type":"journal-article","created":{"date-parts":[[2021,3,21]],"date-time":"2021-03-21T13:13:21Z","timestamp":1616332401000},"page":"85-89","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Expression of CD3 and CD20 in Antistreptolysin-O Titer Seropositive and Seronegative Children with Chronic Tonsillitis"],"prefix":"10.4103","volume":"10","author":[{"given":"Abeer A. K.","family":"Mohamed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahd A.","family":"Alharbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Khalil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-8-20240830","first-page":"566","article-title":"AAO-HNS guidelines for tonsillectomy in children and adolescents","volume":"84","author":"Randel","year":"2011","journal-title":"Am Fam Phys"},{"key":"R2-8-20240830","first-page":"6","article-title":"Moving towards a new era in the research of tonsils and mucosal barriers","volume":"72","author":"Yamanaka","year":"2011","journal-title":"Adv Otorhinolaryngol"},{"key":"R3-8-20240830","doi-asserted-by":"crossref","first-page":"e0183214","DOI":"10.1371\/journal.pone.0183214","article-title":"Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis","volume":"12","author":"Geibler","year":"2017","journal-title":"PLoS One"},{"key":"R4-8-20240830","first-page":"381","article-title":"Chronic tonsillitis: histological and immunohistochemical aspects","volume":"49","author":"Mogoanta","year":"2008","journal-title":"Rom J Morphol Embryol"},{"key":"R5-8-20240830","first-page":"105","article-title":"Antistreptolysin O titer profile in acute rheumatic fever diagnosis","volume":"77","author":"Machado","year":"2001","journal-title":"J Pediatr (Rio J"},{"key":"R6-8-20240830","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1007\/s12070-012-0579-5","article-title":"Evaluation of the validity of high serum antistreptolysin o titre only, as an indication for tonsillectomy","volume":"66","author":"Hembrom","year":"2014","journal-title":"Indian J Otolaryngol Head Neck Surg"},{"key":"R7-8-20240830","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.clindermatol.2007.08.015","article-title":"Triggering psoriasis: the role of infections and medications","volume":"25","author":"Fry","year":"2007","journal-title":"Clinics in Dermatology"},{"key":"R8-8-20240830","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/S1808-8694(15)31279-9","article-title":"Histological analysis of tonsillectomy and adenoidectomy specimens-January 2001 to May 2003","volume":"71","author":"Dell\u2019Aringa","year":"2005","journal-title":"Braz J Otorhinolaryngol"},{"key":"R9-8-20240830","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1440-1754.2005.00726.x","article-title":"New normal ranges of antistreptolysin O and antideoxyribonuclease B titres for Australian children","volume":"41","author":"Danchin","year":"2005","journal-title":"J Paediatr Child Health"},{"key":"R10-8-20240830","doi-asserted-by":"crossref","first-page":"e8","DOI":"10.4081\/pr.2012.e8","article-title":"Antistreptolysin O titer in health and disease: Levels and significance","volume":"4","author":"Kotby","year":"2012","journal-title":"Pediatr Rep"},{"key":"R11-8-20240830","doi-asserted-by":"crossref","first-page":"603","DOI":"10.5935\/1808-8694.20130108","article-title":"Tonsillar hyperplasia and recurrent tonsillitis: clinical-histological correlation","volume":"79","author":"Reis","year":"2013","journal-title":"Braz J Otorhinolaryngol"},{"key":"R12-8-20240830","first-page":"95","article-title":"Chronic tonsillitis can be diagnosed with histopathologic findings","volume":"5","author":"U\u011fra\u015f","year":"2008","journal-title":"Eur J Gen Med"},{"key":"R13-8-20240830","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1001\/archotol.134.5.477","article-title":"Proliferating active cells, lymphocyte subsets, and dendritic cells in recurrent tonsillitis: their effect on hypertrophy","volume":"134","author":"Alatas","year":"2008","journal-title":"Arch Otolaryngol Head Neck Surg"},{"key":"R14-8-20240830","doi-asserted-by":"crossref","first-page":"230","DOI":"10.17305\/bjbms.2013.2330","article-title":"Immunoregulatory cytokines and chronic tonsillitis","volume":"13","author":"Miroljub","year":"2013","journal-title":"Bosn J Basic Med Sci"}],"container-title":["Journal of Microscopy and Ultrastructure"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jmau.jmau_80_20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:21:59Z","timestamp":1787844119000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jmau.jmau_80_20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.4103\/jmau.jmau_80_20","relation":{},"ISSN":["2213-879X"],"issn-type":[{"value":"2213-879X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4]]}}}