{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:20:45Z","timestamp":1792624845726,"version":"4.5.0"},"reference-count":8,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,1]]},"abstract":"\n Introduction:<\/jats:title>\n Cytologic evaluation is the best way to detect the presence of malignancy in body cavity fluids. Roswell Park Memorial Institute (RPMI) medium or RPMI 1640 is used in cell culture, tissue culture, and also to improve the cellularity and morphology of CSF cytology specimens.<\/jats:p>\n <\/jats:sec>\n \n Objectives:<\/jats:title>\n To determine whether RPMI medium can be used to preserve cell morphology in pleural\/peritoneal effusion samples.<\/jats:p>\n <\/jats:sec>\n \n Method and Material:<\/jats:title>\n The study was conducted on 30 pleural\/peritoneal fluid samples received routinely during 2 months for diagnostic purposes in our department. The samples were divided into four parts. One-fourth of the sample was directly refrigerated and the other fourth was at room temperature. In the other two parts, an equal volume of RPMI media was added, and one was kept at room temperature and the other refrigerated. These cytospin-prepared Giemsa-stained smears were examined for cell morphology, cellularity, and occurrence of bacterial colonies at 24 h, 48 h, 72 h, and 1 week, respectively.<\/jats:p>\n <\/jats:sec>\n \n Result:<\/jats:title>\n Refrigerated RPMI medium is the best preservative for pleural\/peritoneal samples; however, samples with RPMI at room temperature were equivalent\/even worse than the simple refrigerated sample.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/joc.joc_130_21","type":"journal-article","created":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T14:22:54Z","timestamp":1682000574000},"page":"26-29","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of Use of RPMI Medium to Preserve Cell Morphology for Pleural\/Peritoneal Fluid Cytology"],"prefix":"10.4103","volume":"39","author":[{"given":"Usha","family":"Rani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meeta","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armaan","family":"Saith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shayama L.","family":"Jain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anurag","family":"Aggarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunita","family":"Aggarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-5-20240905","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/S0272-2712(18)30563-8","article-title":"Effusion cytology","volume":"11","author":"Ehya","year":"1991","journal-title":"Clin Lab Med"},{"key":"R2-5-20240905","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1183\/09031936.97.10020476","article-title":"Diagnostic principles in pleural disease","volume":"10","author":"Light","year":"1997","journal-title":"Eur Respir J"},{"key":"R3-5-20240905","doi-asserted-by":"crossref","first-page":"S163","DOI":"10.1590\/S1806-37132006000900001","article-title":"Coleta e preserva\u00e7\u00e3o do l\u00edquido pleural e bi\u00f3psia pleural [Collection and preservation of the pleural fluid and pleural biopsy]","volume":"32","author":"Antonangelo","year":"2006","journal-title":"J Bras Pneumol"},{"key":"R5-5-20240905","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1001\/jama.1967.03120080053007","article-title":"Culture of normal human leukocytes","volume":"199","author":"Moore","year":"1967","journal-title":"JAMA"},{"key":"R6-5-20240905","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1002\/cncy.21243","article-title":"The addition of RPMI significantly improves the cellularity of cerebrospinal fluid cytology specimens over time","volume":"121","author":"Renshaw","year":"2012","journal-title":"Cancer Cytopathol"},{"key":"R7-5-20240905","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/j.1365-2303.2011.00863.x","article-title":"Effect of temperature and storage time on cellular analysis of fresh pleural fluid samples","volume":"23","author":"Antonangelo","year":"2012","journal-title":"Cytopathology"},{"key":"R8-5-20240905","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/dc.20587","article-title":"Diagnostic effects of prolonged storage on fresh effusion samples","volume":"35","author":"Manosca","year":"2007","journal-title":"Diagn Cytopathol"},{"key":"R9-5-20240905","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1378\/chest.123.4.1181","article-title":"Variations in pleural fluid WBC count and differential counts with different sample containers and different methods","volume":"123","author":"Conner","year":"2003","journal-title":"Chest"}],"container-title":["Journal of Cytology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/joc.joc_130_21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:02:27Z","timestamp":1787760147000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/joc.joc_130_21"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.4103\/joc.joc_130_21","relation":{},"ISSN":["0970-9371"],"issn-type":[{"value":"0970-9371","type":"print"}],"subject":[],"published":{"date-parts":[[2022,1]]}}}