{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:37:47Z","timestamp":1786649867476,"version":"build-2736575974"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Objectives:<\/jats:title>\n                    <jats:p>Among the various biofluids, 8-isoprostane is a relatively underexplored yet stable oxidative stress biomarker that can be detected in measurable quantities. It is a unique by-product of arachidonic acid lipid peroxidation, and in recent years, its expression has been investigated in various diseases, particularly in malignancies. Our study was performed to evaluate the level of 8-isoprostane and compare its expression in plasma and saliva samples of oral leukoplakia (OL), oral submucous fibrosis (OSMF) patients and healthy controls (HC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology:<\/jats:title>\n                    <jats:p>Plasma and saliva samples were collected from clinically and histopathologically confirmed cases of OL, OSMF and HC, with 12 participants in each group. The 8-isoprostane levels in all samples were analysed using the enzyme-linked immunosorbent assay (ELISA) technique.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The expression of 8-isoprostane level was statistically significant (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05) in both plasma and saliva, denoting a steady elevation of 8-isoprostane level in oral potentially malignant disorder (OPMD) (OSMF and OL), when compared to HC. However, when compared to saliva, plasma 8-isoprostane levels were higher in the OL group, whereas the reverse trend was observed in the OSMF group, where salivary levels were higher.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The study reveals a significant plasma and salivary 8-isoprostane level, where the concentration was higher in OPMD groups implying that saliva could be used as the diagnostic sample of choice for evaluating this oxidative stress marker.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jomfp.jomfp_111_25","type":"journal-article","created":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T12:00:08Z","timestamp":1766836808000},"page":"617-621","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Comparative evaluation of Plasma and Salivary 8-isoprostane expression in Oral Leukoplakia and Oral Submucous Fibrosis patients"],"prefix":"10.4103","volume":"29","author":[{"given":"Preethi","family":"Ramesh","sequence":"first","affiliation":[{"name":"Department of Oral Medicine and Radiology, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S Aravind","family":"Warrier","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Radiology, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divyambika C.","family":"Venugopal","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Radiology, Sri Ramachandra Dental College and Hospital, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,12,27]]},"reference":[{"key":"R1-20260420","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J Clin"},{"key":"R2-20260420","doi-asserted-by":"crossref","first-page":"107063","DOI":"10.1016\/j.oraloncology.2024.107063","article-title":"Burden of oral cancer and associated risk factors at national and state levels: A systematic analysis from the global burden of disease in India, 1990\u20132021","volume":"159","author":"Zahiruddin","year":"2024","journal-title":"Oral Oncol"},{"key":"R3-20260420","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/s41571-021-00514-z","article-title":"Planning for tomorrow: Global cancer incidence and the role of prevention 2020-2070","volume":"18","author":"Soerjomataram","year":"2021","journal-title":"Nat Rev Clin Oncol"},{"issue":"Suppl 3","key":"R4-20260420","doi-asserted-by":"crossref","first-page":"S2794","DOI":"10.4103\/jpbs.jpbs_376_24","article-title":"Prevalence of potentially malignant disorders in tobacco consuming population: A cross-sectional analysis","volume":"16","author":"Shaikh","year":"2024","journal-title":"J Pharm Bioallied Sci"},{"key":"R5-20260420","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.1111\/odi.13704","article-title":"Oral potentially malignant disorders: A consensus report from an international seminar on nomenclature and classification, convened by the WHO Collaborating Centre for Oral Cancer","volume":"27","author":"Warnakulasuriya","year":"2021","journal-title":"Oral Dis"},{"key":"R6-20260420","doi-asserted-by":"crossref","first-page":"5211","DOI":"10.21873\/anticanres.12844","article-title":"Biomarkers of oxidative stress associated with the risk of potentially malignant oral disorders","volume":"38","author":"Senghore","year":"2018","journal-title":"Anticancer Res"},{"key":"R7-20260420","doi-asserted-by":"crossref","first-page":"279","DOI":"10.4103\/jomfp.JOMFP_235_19","article-title":"8-Isoprostane: A salivary oxidative stress biomarker for oral submucous fibrosis and oral squamous cell carcinoma","volume":"24","author":"Meera","year":"2020","journal-title":"J Oral Maxillofac Pathol"},{"key":"R8-20260420","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1007\/s12291-017-0689-7","article-title":"Correlation of clinical and histopathological grades in oral submucous fibrosis patients with oxidative stress markers in saliva","volume":"33","author":"Divyambika","year":"2018","journal-title":"Indian J Clin Biochem"},{"key":"R9-20260420","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0006-2952(95)02072-1","article-title":"The isoprostanes. Current knowledge and directions for future research","volume":"51","author":"Morrow","year":"1996","journal-title":"Biochem Pharmacol"},{"key":"R10-20260420","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1177\/0022034511420433","article-title":"Scientific frontiers: emerging technologies for salivary diagnostics","volume":"23","author":"Baum","year":"2011","journal-title":"Adv Dent Res"},{"key":"R11-20260420","doi-asserted-by":"crossref","first-page":"3757","DOI":"10.1007\/s12070-023-04076-7","article-title":"Oxidative stress enzyme as markers in oral potentially malignant disorders and oral squamous cell carcinoma patients","volume":"75","author":"Shamsi","year":"2023","journal-title":"Indian J Otolaryngol Head Neck Surg"},{"key":"R12-20260420","doi-asserted-by":"crossref","first-page":"102073","DOI":"10.1016\/j.jebdp.2024.102073","article-title":"Oxidative stress and antioxidant markers in oral submucous fibrosis: a systematic review and meta-analysis of case-control studies","volume":"25","author":"Guo","year":"2025","journal-title":"J Evid Based Dent Pract"},{"key":"R13-20260420","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1158\/1940-6207.CAPR-09-0151","article-title":"Overexpression of cyclooxygenase-2 in rat oral cancers and prevention of oral carcinogenesis in rats by selective and nonselective COX inhibitors","volume":"3","author":"McCormick","year":"2010","journal-title":"Cancer Prev Res (Phila)"},{"key":"R14-20260420","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.oraloncology.2013.09.011","article-title":"Oxidative and antioxidative mechanisms in oral cancer and precancer: A review","volume":"50","author":"Choudhari","year":"2014","journal-title":"Oral Oncol"},{"key":"R15-20260420","doi-asserted-by":"crossref","first-page":"3115","DOI":"10.1111\/bph.12677","article-title":"Pathophysiology of isoprostanes in the cardiovascular system: Implications of isoprostane\u2010mediated thromboxane A 2 receptor activation","volume":"171","author":"Bauer","year":"2014","journal-title":"Br J Pharmacol"},{"key":"R16-20260420","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5487\/TR.2011.27.1.001","article-title":"Lipid peroxidation and its toxicological implications","volume":"27","author":"Nam","year":"2011","journal-title":"Toxicol Res"},{"key":"R17-20260420","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/s12663-011-0195-z","article-title":"Oral cancer: Risk factors and molecular pathogenesis","volume":"10","author":"Ram","year":"2011","journal-title":"J Maxillofac Oral Surg"},{"key":"R18-20260420","first-page":"20","article-title":"Smoking induced oxidative stress in serum and neutrophil of the university students","volume":"1","author":"Mahapatra","year":"2008","journal-title":"Al Ameen J Med Sci"},{"key":"R21-20260420","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41368-023-00231-6","article-title":"Salivary biomarkers: novel noninvasive tools to diagnose chronic inflammation","volume":"15","author":"Dongiovanni","year":"2023","journal-title":"Int J Oral Sci"},{"key":"R22-20260420","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1111\/j.1600-0714.2012.01143.x","article-title":"Role of free radicals and antioxidant defences in oral cavity-related pathologies","volume":"41","author":"Iannitti","year":"2012","journal-title":"J Oral Pathol Med"},{"key":"R23-20260420","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1056\/NEJM199505043321804","article-title":"Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers\u2014smoking as a cause of oxidative damage","volume":"332","author":"Morrow","year":"1995","journal-title":"N Engl J Med"},{"key":"R24-20260420","doi-asserted-by":"crossref","first-page":"5846","DOI":"10.3748\/wjg.v12.i36.5846","article-title":"Improved method of plasma 8-Isoprostane measurement and association analyses with habitual drinking and smoking","volume":"12","author":"Kitano","year":"2006","journal-title":"World J Gastroenterol"},{"key":"R25-20260420","doi-asserted-by":"crossref","first-page":"113","DOI":"10.4103\/jofs.jofs_220_20","article-title":"Evaluation of Salivary 8-Isoprostane in oral lichen planus: Case control study","volume":"12","author":"Dinesan","year":"2020","journal-title":"J Orofac Sci"}],"container-title":["Journal of Oral and Maxillofacial Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jomfp.jomfp_111_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T18:59:20Z","timestamp":1786647560000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jomfp.jomfp_111_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/jomfp.jomfp_111_25","relation":{},"ISSN":["0973-029X","1998-393X"],"issn-type":[{"value":"0973-029X","type":"print"},{"value":"1998-393X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-04-17","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-28","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}