{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:24:59Z","timestamp":1787772299772,"version":"build-2784847793"},"reference-count":13,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,10]]},"abstract":"Abstract<\/jats:title>\n \n Background:<\/jats:title>\n \n Recent researches has shown a significant association between microorganisms and oral squamous cell carcinoma (OSCC).\n Porphyromonas gingivalis<\/jats:italic>\n , the keystone pathogen in chronic periodontitis, is considered as an important potential etiologic agent of OSCC, but the underlying mechanisms by which\n P. gingivalis<\/jats:italic>\n mediates OSCC progression remain poorly understood.\n <\/jats:p>\n <\/jats:sec>\n \n Aim:<\/jats:title>\n \n The aim of this study was to compare the levels of\n P. gingivalis<\/jats:italic>\n in oral potentially malignant disorders, oral squamous cell carcinoma and normal oral mucosa using qRT-PCR.\n <\/jats:p>\n <\/jats:sec>\n \n Method and Material:<\/jats:title>\n \n Genomic DNA was extracted and quantified, and the expression of the\n P. gingivalis<\/jats:italic>\n levels was done in 16 cases of oral potentially malignant disorders, 16 cases of oral squamous cell carcinoma and 16 cases of normal oral mucosa by quantitative real-time polymerase chain reaction (RT-qPCR).\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n It was observed that there was an over expression of\n P. gingivalis<\/jats:italic>\n in both oral potentially malignant disorders and oral squamous cell carcinoma with good mean cycle threshold (CT) value of 27.00 and 27.55, respectively. When comparing the levels of\n P. gingivalis<\/jats:italic>\n in three groups, oral potentially malignant disorders (OPMD) and oral squamous cell carcinoma (OSCC) showed higher expression than normal mucosa and in between two groups OSCC showed higher expression than OPMD and the difference is statistically significant with\n P<\/jats:italic>\n value less than 0.001.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n Our findings suggest that there is an over expression of\n P. gingivalis<\/jats:italic>\n in oral potentially malignant disorders and oral squamous cell carcinoma, compared to normal mucosa and highly expressed in OSCCs compared to OPMD. Increased levels of\n P. gingivalis<\/jats:italic>\n in OPMDs and OSCCs may suggest the early event of tumorigenesis. Hence, it can be used as a valuable marker for early diagnosis, prognosis marker and in the identification of therapeutic targets.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jomfp.jomfp_119_24","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T08:01:21Z","timestamp":1735632081000},"page":"583-588","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Detection of porphyromonas gingivalis in oral potentially malignant disorders and oral squamous cell carcinoma using qRT-PCR: A comparative study"],"prefix":"10.4103","volume":"28","author":[{"given":"G","family":"Suganya","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Government Dental College and Research Institute, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NS","family":"Sahana","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Government Dental College and Research Institute, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Akalya","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Government Dental College and Research Institute, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish T.","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Government Dental College and Research Institute, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Suresh","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Government Dental College and Research Institute, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Chandrakala","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Government Dental College and Research Institute, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,12,31]]},"reference":[{"key":"R1-20250108","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1177\/0022034520909312","article-title":"Porphyromonas gingivalis promotes oral squamous cell carcinoma progression in an immune microenvironment","volume":"99","author":"Wen","year":"2020","journal-title":"J Dent Res"},{"key":"R2-20250108","doi-asserted-by":"crossref","first-page":"24","DOI":"10.21608\/mjd.2020.198725","article-title":"Detection of the periodontal pathogen Porphyromonas gingivalis in oral squamous cell carcinoma","volume":"7","author":"Monier","year":"2020","journal-title":"Mansoura J 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between Porphyromonas gingivalis and oral squamous cell carcinoma,\u201d in Summary of the 10th national conference on periodontal disease, Changchun, China","author":"Chang","year":"2014"}],"container-title":["Journal of Oral and Maxillofacial 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