{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:21:13Z","timestamp":1787775673176,"version":"build-2784847793"},"reference-count":45,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The focus of caries research has switched to early identification and non-invasive treatment of carious lesions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>\n                      This study aimed to evaluate and compare the remineralising potential of\n                      <jats:italic toggle=\"yes\">Ocimum (O.) basilicum<\/jats:italic>\n                      varnish and fluoride varnish on initial enamel caries.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method:<\/jats:title>\n                    <jats:p>\n                      The authenticated\n                      <jats:italic toggle=\"yes\">O. basilicum<\/jats:italic>\n                      seeds were procured from a repository, and the extract was prepared using the Soxhlet method, which was vortexed with Indian Pharmaceutical (IP)-graded chemicals to obtain varnish. Extracted premolar tooth samples were divided into three groups of 33 each after demineralisation with a pH of 4.5 for 48 hours at 37\u00b0C. Each group was subjected to remineralisation twice daily with respective agents for 4 minutes for 30 consecutive days. Each sample was ground-sectioned through an enamel window. The lesion depth was measured using a light microscope (Leica\u2122 DM2500) and ImageJ software. The data were evaluated using analysis of variance (ANOVA) and post hoc analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The mean (\u00b1 SD) pre-treatment lesion depth across the groups ranged from 242.11 \u00b1 26.144 \u03bcm to 352.66 \u00b1 34.531 \u03bcm. The highest lesion depth recovery rate of 45.938% was recorded for the fluoride varnish group, followed by 36.015% in the\n                      <jats:italic toggle=\"yes\">O. basilicum<\/jats:italic>\n                      varnish group, which was statistically significant by Tukey\u2019s post hoc analysis (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.001). The gingival fibroblast cells were viable by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic toggle=\"yes\">O. basilicum<\/jats:italic>\n                      varnish demonstrated a homogenous layer of mineral deposition. However, the remineralising efficacy was slightly lesser than that of the fluoride varnish. Hence, the novel\n                      <jats:italic toggle=\"yes\">O. basilicum<\/jats:italic>\n                      -based remineralisation agent appears to have potential as a non-invasive alternative to topical fluorides in the therapy of early caries lesions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jomfp.jomfp_174_23","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T23:01:04Z","timestamp":1703113264000},"page":"776-777","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":5,"title":["Remineralising potential of Ocimum basilicum varnish and fluoride varnish on initial enamel caries: An in vitro microscopic study"],"prefix":"10.4103","volume":"27","author":[{"given":"Atrey J.","family":"Pai Khot","sequence":"first","affiliation":[{"name":"Department of Public Health Dentistry, KLE Vishwanath Katti Institute of Dental Sciences, KLE Academy of 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India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram Surath","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Public Health Dentistry, KLE Vishwanath Katti Institute of Dental Sciences, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehul A.","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Public Health Dentistry, KLE Vishwanath Katti Institute of Dental Sciences, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,12,20]]},"reference":[{"key":"R1-20260302","doi-asserted-by":"crossref","first-page":"45","DOI":"10.4103\/INPC.INPC_6_19","article-title":"Comparative study of remineralization potential of three different remineralizing agents on demineralized enamel using light fluorescence 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