{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:41:31Z","timestamp":1787071291410,"version":"build-2736575974"},"reference-count":13,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,5]]},"abstract":"\n Background:<\/jats:title>\n \n Oral submucous fibrosis (OSMF) is a chronic debilitating condition of the oral mucosa that has been classified as a potentially malignant disorder with a malignant transformation rate of 2%\u20138%. Several\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n experiments have been performed to formulate a treatment modality for OSMF, yet no ideal\n in vitro<\/jats:italic>\n primary oral fibroblast model has been developed.\n <\/jats:p>\n <\/jats:sec>\n \n Aim:<\/jats:title>\n \n To establish an\n in vitro<\/jats:italic>\n primary oral fibroblast model.\n <\/jats:p>\n <\/jats:sec>\n \n Setting and Design:<\/jats:title>\n \n In vitro<\/jats:italic>\n laboratory setting.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methodology:<\/jats:title>\n Primary cell culture protocol was performed after obtaining normal oral tissue. Karyotyping was performed to rule out chromosomal abnormalities. Immunofluorescence staining was carried with a panel of fibroblast-specific markers (vimentin, phalloidin, transforming growth factor-\u03b2 receptor 1 [TGF\u03b2R1] and s100a4) and Masson trichrome staining (MTS) to demonstrate the presence of extracellular matrix (ECM) qualitatively.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n A monolayer of oral fibroblasts was observed on the 9\n th<\/jats:sup>\n -day postseeding. No chromosomal abnormality was observed in the patient samples. Positive staining was observed with vimentin, phalloidin, TGF\u03b2R1 and s100a4, thereby confirming the cell type. MTS revealed fibroblasts with spindle morphology and scanty ECM.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n The present study lays down a protocol to design and characterize primary buccal fibroblast cell culture model that would aid researchers in performing\n in vitro<\/jats:italic>\n preliminary experiments in areas concerning fibrosis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jomfp.jomfp_28_19","type":"journal-article","created":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T08:27:16Z","timestamp":1566980836000},"page":"198-202","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":10,"title":["Characterization of oral fibroblasts: An in vitro model for oral fibrosis"],"prefix":"10.4103","volume":"23","author":[{"given":"Pooja","family":"Adtani","sequence":"first","affiliation":[{"name":"Department of Oral Pathology, College of Dentistry, Gulf Medical University, Ajman, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malathi","family":"Narasimhan","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kannan","family":"Ranganathan","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Ragas Dental College, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Punnoose","sequence":"additional","affiliation":[{"name":"Research Center for Regenerative Medicine and Stem Cell Research, Central Research Facility, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, 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