{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T14:24:31Z","timestamp":1783347871854,"version":"3.54.6"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,4]]},"abstract":"Abstract<\/jats:title>\n \n Purpose:<\/jats:title>\n Oral squamous cell carcinoma (OSCC) concomitant with oral submucous fibrosis (OSCC-OSMF) is a clinico-pathologically distinct entity. Myofibroblasts (MFs) and its differentiation plays pivotal role in tumour progression via cell cycle alterations, epithelial\u2013mesenchymal transition (EMT), hypoxia, immunomodulatory disorders, oxidative injury and epigenetic modifications.<\/jats:p>\n <\/jats:sec>\n \n Objective:<\/jats:title>\n \n The present study evaluated immunohistochemical expression of\n AKT3<\/jats:italic>\n and alpha-smooth muscle actin amongst, OSMF and OSCC-OSMF and correlated with related clinicopathological parameters.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n 70 formalin-fixed paraffin-embedded tissue samples of histopathologically confirmed cases, including 10 NOM, 30 OSMF, and 30 OSCC-OSMF, were immunohistochemically stained with\n AKT3<\/jats:italic>\n and (\u03b1-SMA) antibodies to quantify MFs and myofibroblastic cancer associated myofibroblasts (myCAFs).\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The percentage of \u03b1-SMA and\n AKT3<\/jats:italic>\n positive cells was statistically significant within the OSMF and OSCC-OSMF study groups (\n P<\/jats:italic>\n < 0.0001). A progressive increase in cytoplasmic expression of \u03b1-SMA and\n AKT3<\/jats:italic>\n was observed from NOM to OSMF and OSCC-OSMF. A statistically significant correlation was found between the stages of OSMF and the clinical TNM stages of OSCC-OSMF.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n Increased stromal expression of \u03b1-SMA and\n AKT3<\/jats:italic>\n is associated with disease progression in OSMF and OSCC-OSMF. This is the first study to investigate\n AKT-3<\/jats:italic>\n expression in OSMF and OSCC arising in OSMF, and these markers may serve as potential indicators of stromal activation and tumour progression, thereby warranting further longitudinal and functional investigations.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jomfp.jomfp_400_25","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T18:00:15Z","timestamp":1783101615000},"page":"186-193","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["AKT3 and \u03b1-SMA in oral submucous fibrosis and oral squamous cell carcinoma with concomitant oral submucous fibrosis as potential prognostic indicators"],"prefix":"10.4103","volume":"30","author":[{"given":"Sonali","family":"Behera","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, SCB Govt. Dental College and Hospital, Cuttack, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachna","family":"Rath","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, SCB Govt. Dental College and Hospital, Cuttack, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surya Narayan","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, SCB Govt. Dental College and Hospital, Cuttack, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aishwarya","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, SCB Govt. 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