{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:35:45Z","timestamp":1787841345573,"version":"build-2784847793"},"reference-count":19,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec>\n                    <jats:title>Context:<\/jats:title>\n                    <jats:p>Hypoxia-inducible factor (HIF)-2\u03b1 is overexpressed in primary and metastatic human cancers, whose expression is correlated with tumor angiogenesis and patient mortality. HIF plays a role in the progression of fibrosis in oral submucous fibrosis (OSF).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim and Objective:<\/jats:title>\n                    <jats:p>The aim is to study and compare the expression of HIF-2\u03b1 in OSF (a), oral squamous cell carcinoma (OSCC) with areca nut usage (b), OSCC without areca nut usage (c) and normal mucosa (d) by immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Subjects and Methods:<\/jats:title>\n                    <jats:p>Immunohistochemical detection of HIF-2\u03b1 was done on 51 samples, which included 11 cases (a), 15 cases (b), 15 cases (c) and the expression was compared with that of (d).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Statistical Analysis<\/jats:title>\n                    <jats:p>\n                      Data were analyzed using the SPSS\u2122 software (ver. 21.0). Chi-square test and kappa analysis were performed to compare the intensity of staining between the groups and for inter-observer agreement, respectively. Value of\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2264 0.05 was considered statistically significant. The mean labeling index between the groups was studied by the Kruskal\u2013Wallis test.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      All the cases of (d), (a), (b) and (c) showed HIF-2\u03b1 expression (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.329). About 13% cases of (c) showed intense expression (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.406) and 27% of (a) cases showed expression only in the connective tissue (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.023). The number of positively stained nuclei in both (b and c) cases reduced as the tumor progression was from well to poorly differentiated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Areca nut initiates fibrosis and subsequent hypoxia in OSF which triggers HIF-2\u03b1 expression in the epithelium. HIF-2\u03b1 could be a surrogate marker for cancer initiation and progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jomfp.jomfp_42_19","type":"journal-article","created":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T15:47:24Z","timestamp":1588866444000},"page":"33-39","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":7,"title":["Study of Hypoxia-inducible factor-2\u03b1 expression in the malignant transformation of Oral submucous fibrosis"],"prefix":"10.4103","volume":"24","author":[{"given":"Immanuel","family":"Joseph","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Elizabeth","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospital, 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