{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:40:53Z","timestamp":1786653653924,"version":"build-2736575974"},"reference-count":6,"publisher":"Ovid Technologies (Wolters Kluwer Health)","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Assessing the prevalence of exfoliated oral mucosal cell micronuclei in tobacco smokers, healthy persons, and patients with potentially malignant diseases (PMDs). A critical comparative investigation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Procedures:<\/jats:title>\n                    <jats:p>The study included 80 participants divided into four groups: healthy persons, tobacco users without any clinically noticeable oral lesions, patients with leukoplakia, and patients with oral submucous fibrosis (OSMF). Each group included 20 participants. Only cases with a verified histological diagnosis of PMDs were considered for evaluating micronuclei (MNi). This included non-smokers with mucosa that seems normal, smokers with mucosa that appears normal, and individuals with leukoplakia and OSMF who were histologically identified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Compared to controls, smokers and patients with PMDs had much higher MN frequencies, with PMD patients having the highest MN frequency. There was a noticeable rise in MN frequency in the H and E and PAP stains from controls to tobacco users and then to PMD patients, showing a stepwise development. While the difference between tobacco users and PMD patients was only significant with the H and E stain, the inter-group comparisons show significant differences in MN frequency between controls and both tobacco users and PMD patients across both staining techniques. Additionally, the comparison of the H and E and PAP stains reveals that whereas MN frequencies in smokers and controls are typically similar, PMD patients have considerably higher MN frequencies in the H and E stains.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>In summary, the research indicates that tobacco users have a much higher MN frequency than controls, with an even more pronounced in individuals who have PMDs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_1074_24","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T02:00:12Z","timestamp":1732068012000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Exfoliated Oral Mucosal Cell Micronucleus Frequency Evaluation in Healthy Individuals, Tobacco Users, and Patients with Potentially Malignant Disorders: An Analytical Comparative Study"],"prefix":"10.4103","author":[{"given":"V","family":"Kranthi","sequence":"first","affiliation":[{"name":"Department of Oral Pathology and Microbiology, Malla Reddy Institute of Dental Sciences, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Shylaja","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology, SVS Institute of Dental Sciences, Mahabubnagar, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallavi","family":"Kandi","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Radiology, Panineeya Mahavidyalaya Institute of Dental Sciences and Research Centre, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Abidullah","sequence":"additional","affiliation":[{"name":"Department of Biomedical Dental Sciences, Faculty of Dentistry, Al Baha University, Al Baha, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neeharika","family":"Soorneedi","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology, Malla Reddy Institute of Dental Sciences, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tummakomma P.","family":"Latha","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Malla Reddy Institute of Dental Sciences, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"key":"R1-20250114","first-page":"15","article-title":"Comparative study of exfoliated oral mucosal cell micronucleus frequency in potentially malignant and malignant lesions","volume":"3","author":"Dindgire","year":"2012","journal-title":"Int J Oral Maxillofac Pathol"},{"key":"R2-20250114","first-page":"197","article-title":"Micronucleus as potential biomarker of oral carcinogenesis","volume":"2","author":"Jois","year":"2010","journal-title":"Indian J Dent Adv"},{"key":"R3-20250114","doi-asserted-by":"crossref","first-page":"230","DOI":"10.4103\/0970-9371.103940","article-title":"A comparative study for selectivity of micronuclei in oral exfoliated epithelial cells","volume":"29","author":"Grover","year":"2012","journal-title":"J Cytol"},{"key":"R4-20250114","doi-asserted-by":"crossref","first-page":"201","DOI":"10.4317\/jced.3.e201","article-title":"Comparative study of oral micronucleated cell frequency in oral submucous fibrosis patients and healthy individuals","volume":"3","author":"Anila","year":"2011","journal-title":"J Clin Exp Dent"},{"key":"R5-20250114","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00784-006-0075-y","article-title":"Micronucleus-an upcoming marker of genotoxic damage","volume":"11","author":"Kamboj","year":"2007","journal-title":"Clin Oral Investig"},{"key":"R7-20250114","doi-asserted-by":"crossref","first-page":"165","DOI":"10.4103\/0970-9371.86342","article-title":"Efficacy of oral brush cytology in the evaluation of oral premalignant and malignant lesions","volume":"28","author":"Babshet","year":"2011","journal-title":"J Cytol"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_1074_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:40:31Z","timestamp":1786650031000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_1074_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,19]]},"references-count":6,"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_1074_24","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,19]]},"assertion":[{"value":"2024-08-21","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-12","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}