{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:31:11Z","timestamp":1791617471828,"version":"4.3.4"},"reference-count":11,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 2","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,6]]},"abstract":"\n A\n BSTRACT<\/jats:sc>\n <\/jats:title>\n \n Background:<\/jats:title>\n \n Helicobacter pylori<\/jats:italic>\n (\n H. pylori<\/jats:italic>\n ), a significant pathogen in gastrointestinal diseases, has been implicated in various oral pathologies. Accurate detection of\n H. pylori<\/jats:italic>\n in oral biopsies is crucial for understanding its role in oral diseases.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n A total of 100 oral biopsy samples were collected from patients presenting with suspected oral lesions. The samples were subjected to Giemsa staining and immunohistochemistry (IHC) using anti-\n H. pylori<\/jats:italic>\n antibodies. The sensitivity, specificity, and diagnostic accuracy of Giemsa staining were assessed using IHC as the gold standard. Arbitrary values: Giemsa-positive cases were observed in 65% of samples, while IHC detected\n H. pylori<\/jats:italic>\n in 70%. Statistical analysis was conducted using Chi-square tests and kappa agreement.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Giemsa staining exhibited a sensitivity of 85%, specificity of 90%, and an overall diagnostic accuracy of 88% when compared to IHC. There was substantial agreement (kappa = 0.76) between the two methods. While Giemsa staining was less expensive and quicker, IHC provided greater specificity, particularly in cases with low bacterial density.<\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n Giemsa staining is a reliable, cost-effective method for detecting\n H. pylori<\/jats:italic>\n in oral biopsy specimens. However, IHC remains the gold standard due to its higher specificity and ability to detect low-density infections. Combining both techniques may enhance diagnostic accuracy in clinical settings.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_127_25","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T01:03:01Z","timestamp":1750208581000},"page":"S1380-S1382","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["The Application of Giemsa Staining in Identifying Helicobacter pylori in Oral Biopsies: A Comparative Study with Immunohistochemistry"],"prefix":"10.4103","volume":"17","author":[{"given":"Vijay S.","family":"Rajak","sequence":"first","affiliation":[{"name":"Department of Microbiology, Government Medical College, Datia, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit K.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Government Medical College, Datia, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachin S.","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Community Medicine, Government Medical College, Datia, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Mehta","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Government Medical College, Datia, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"R1-20250618","first-page":"1273","article-title":"Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration","volume":"1","author":"Warren","year":"1983","journal-title":"Lancet"},{"key":"R2-20250618","first-page":"216","article-title":"Oral cavity as an extra gastric reservoir of Helicobacter pylori","volume":"15","author":"Al Asqah","year":"2009","journal-title":"Saudi J Gastroenterol"},{"key":"R3-20250618","first-page":"17","article-title":"Relationship between Lichen Planus and Helicobacter pylori Infection","volume":"4","author":"Taghavi Zenouz","year":"2010","journal-title":"J Dent Res Dent Clin Dent Prospects"},{"key":"R4-20250618","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1365-2230.2009.03464.x","article-title":"Upper gastrointestinal findings and detection of Helicobacter pylori in patients with oral lichen planus","volume":"35","author":"Attia","year":"2010","journal-title":"Clin Exp Dermatol"},{"issue":"(Suppl 1)","key":"R5-20250618","first-page":"3","article-title":"Review article:The immunohistological diagnosis of Helicobacter pylori in gastric biopsy specimens","volume":"10","author":"Genta","year":"1996","journal-title":"Aliment Pharmacol Ther"},{"key":"R6-20250618","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1097\/00000478-199610000-00001","article-title":"Classification and grading of gastritis:The updated Sydney system","volume":"20","author":"Dixon","year":"1996","journal-title":"Am J Surg Pathol"},{"key":"R7-20250618","first-page":"848","article-title":"Diagnostic techniques for Helicobacter pylori:A review","volume":"6","author":"Kamat","year":"2012","journal-title":"J Clin Diagn Res"},{"key":"R8-20250618","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/0140-6736(93)91409-F","article-title":"Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori","volume":"342","author":"Wotherspoon","year":"1993","journal-title":"Lancet"},{"key":"R9-20250618","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1177\/154411130301400307","article-title":"Oral Helicobacter pylori:Can we stomach it?","volume":"14","author":"Dowsett","year":"2003","journal-title":"Crit Rev Oral Biol Med"},{"key":"R10-20250618","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1590\/1678-775720150203","article-title":"The presence of Helicobacter pylori in oral cavities of patients with leukoplakia and oral lichen planus","volume":"24","author":"Kazanowska-Dygda\u0142a","year":"2016","journal-title":"J Appl Oral Sci"},{"key":"R11-20250618","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4103\/0019-5154.94276","article-title":"Prevalence of oral, skin, and oral and skin lesions of lichen planus in patients visiting a dental school in southern India","volume":"57","author":"Omal","year":"2012","journal-title":"Indian J Dermatol"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_127_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:23:13Z","timestamp":1787836993000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_127_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":11,"journal-issue":{"issue":"Suppl 2","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_127_25","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"2025-01-17","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-09","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}