{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:26:32Z","timestamp":1787840792076,"version":"build-2784847793"},"reference-count":8,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 5","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,12]]},"abstract":"\n A\n BSTRACT<\/jats:sc>\n <\/jats:title>\n \n Aim and Objectives:<\/jats:title>\n Age and gender identification are critical in forensic investigations, especially for unidentified human bodies. This study explores differences in nuclear size, nuclear area, and nuclear-cytoplasmic ratio between healthy individuals and those with physiological or pathological conditions, aiming to understand how these variations may influence age estimation.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n Following ethical clearance, the study was conducted on 80 participants aged 20\u201335 years, who were divided into four groups of 20 each, Group 1: Healthy individuals; Group 2: Individuals with physiological variations; Group 3: Individuals with pathological variations; and Group 4: Individuals with acquired conditions. Buccal cell samples were collected using a moistened tongue blade. Measurements of cell size, nuclear dimensions, nuclear area, and the nuclear-cytoplasmic ratio were taken.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Significant differences in nuclear and cell sizes were observed among participants with acquired conditions and metabolic disorders compared to healthy controls.<\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n The study suggests that systemic diseases and tobacco use (without visible oral lesions) can significantly affect cellular morphology, which may have implications for the accuracy of age determination in forensic analyses.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_1351_24","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T11:05:27Z","timestamp":1738235127000},"page":"S4861-S4863","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Accuracy and Reliability of Oral Exfoliative Cytology in Age Estimation: An Observational Study"],"prefix":"10.4103","volume":"16","author":[{"given":"Naseemoon","family":"Shaik","sequence":"first","affiliation":[{"name":"Department of Pedodontics and Preventive Dentistry, MNR Dental College and Hospital, Sangareddy, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Anne","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Laramie Pediatrics and Internal Medicine pc, Wyoming, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saraswathi Saisupraja","family":"Mantha","sequence":"additional","affiliation":[{"name":"Department of Epidemiology (MPH), St. Joseph\u2019s Dental College and Hospital, Eluru, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Medapati Murali Jogi","family":"Reddy","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology, Lenora Institute of Dental Sciences, Rajanagaram, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidya","family":"Priyadarshini","sequence":"additional","affiliation":[{"name":"Department of Periodontics, JSS Dental College and Hospital, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Tanuja","sequence":"additional","affiliation":[{"name":"Department of Periodontics, G. Pulla Reddy Dental College and Hospital, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sriharsha","family":"Pudi","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Crown and Bridge, MNR Dental College and Hospital, Sangareddy, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"R1-20250130","first-page":"22","article-title":"Cytomorphometric analysis of normal exfoliative cells from buccal mucosa in different age groups","volume":"2","author":"Reddy","year":"2011","journal-title":"Int J Clin Dent Sci"},{"key":"R2-20250130","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1002\/dc.23941","article-title":"New biological research and understanding of Papanicolaou's test","volume":"46","author":"Smith","year":"2018","journal-title":"Diagn Cytopathol"},{"key":"R3-20250130","doi-asserted-by":"crossref","first-page":"63","DOI":"10.4103\/0975-1475.150321","article-title":"Exfoliative cytology:A possible tool in age estimation in forensic odontology","volume":"7","author":"Shetty","year":"2015","journal-title":"J Forensic Dent Sci"},{"key":"R4-20250130","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1002\/dc.22887","article-title":"A brief chronicle of cytology:from Janssen to Papanicolaou and beyond","volume":"41","author":"Diamantis","year":"2013","journal-title":"Diagnostic cytopathology"},{"key":"R5-20250130","first-page":"9","article-title":"A review of most commonly used dental age estimation technique","volume":"19","author":"Willems","year":"2001","journal-title":"J. Forensic Odonto Stomatol"},{"key":"R6-20250130","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1177\/00220345650440040301","article-title":"Effects of race, age, smoking habits, oral and systemic disease on oral exfoliative cytology","volume":"44","author":"Zimmermann","year":"1965","journal-title":"J Dent Res"},{"key":"R7-20250130","first-page":"205","article-title":"The cytomorphological analysis of buccal mucosa cells in smokers","volume":"41","author":"G\u00f6regen","year":"2011","journal-title":"Turk J Med Sci"},{"key":"R8-20250130","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/j.1600-0714.1999.tb02108.x","article-title":"The effect of tobacco smoking and of betel chewing with tobacco on the buccal mucosa:A cytomorphometric analysis","volume":"28","author":"Ramaesh","year":"1999","journal-title":"J Oral Pathol Me"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_1351_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:28:57Z","timestamp":1787837337000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_1351_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":8,"journal-issue":{"issue":"Suppl 5","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_1351_24","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"2024-09-25","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-16","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}