{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:44:26Z","timestamp":1787071466175,"version":"build-2736575974"},"reference-count":8,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 4","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,12]]},"abstract":"\n Background:<\/jats:title>\n Promoter hypermethylation of tumor suppressor genes is a hallmark of early carcinogenesis in oral lesions.<\/jats:p>\n <\/jats:sec>\n \n Aim:<\/jats:title>\n \n To assess the methylation status of\n p16<\/jats:italic>\n ,\n DAPK<\/jats:italic>\n , and\n MGMT<\/jats:italic>\n in oral leukoplakia and OSMF.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n DNA extracted from tissue samples of 30 leukoplakia, 30 OSMF, and 20 healthy controls was analyzed using methylation-specific PCR.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Promoter hypermethylation was significantly higher in both patient groups compared to controls (\n P<\/jats:italic>\n < 0.05), with\n p16<\/jats:italic>\n most frequently affected.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n Aberrant promoter methylation may serve as an early molecular marker in oral precancerous conditions, aiding early intervention and risk stratification.<\/jats:p>\n 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