{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:26:25Z","timestamp":1787840785546,"version":"build-2784847793"},"reference-count":12,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 5","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>To evaluate the IL-35 Gene Polymorphism in Periodontitis subjects with and without type 2 diabetes mellitus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology:<\/jats:title>\n                    <jats:p>\n                      96 participants were categorized into three groups, Group 1: Systemically and Periodontally healthy individuals, Group 2: Systemically healthy patients with Generalized chronic periodontitis, Group 3: Generalized chronic periodontitis with type 2 diabetes Mellitus. Genomic DNA was extracted from the whole venous blood samples collected from the participants using The PureLink Genomic Kit. The region spanning\n                      <jats:italic toggle=\"yes\">rs1580809257<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">rs1580801731 were<\/jats:italic>\n                      amplified using appropriate primer and reaction conditions. Determination of genotypes for each of the polymorphisms assessed using Amplification Refractory Mutation System (ARMS) - based PCR and Restriction Fragment Length Polymorphism (RFLP). The distribution of genotype and allele frequencies for Case and control groups were compared using the Chi-square test. Hardy Weinberg equilibrium is calculated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The gene and allele frequency were found to be similar in all three groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      The study reveals that polymorphism of\n                      <jats:italic toggle=\"yes\">rs1580809257<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">rs1580801731<\/jats:italic>\n                      in the IL-35 gene is neither associated with Periodontitis patients nor Periodontitis with Diabetes patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_1483_24","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T11:04:28Z","timestamp":1738235068000},"page":"S4826-S4831","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Assessment of IL- 35 Gene Polymorphism in Periodontitis Patients with and without Diabetes - A Cross-Sectional Study"],"prefix":"10.4103","volume":"16","author":[{"given":"J Sri Vishnu","family":"Durga","sequence":"first","affiliation":[{"name":"Department of Periodontology, KSR Institute of Dental Science and Research, Tiruchengode, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R Renuka","family":"Devi","sequence":"additional","affiliation":[{"name":"Department of Periodontology, KSR Institute of Dental Science and Research, Tiruchengode, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Sonika","sequence":"additional","affiliation":[{"name":"Department of Periodontology, KSR Institute of Dental Science and Research, Tiruchengode, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Vignesh","sequence":"additional","affiliation":[{"name":"Department of Periodontology, KSR Institute of Dental Science and Research, Tiruchengode, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"R1-20250130","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1600-0757.2005.00118.x","article-title":"The genetic basis of periodontitis","volume":"39","author":"Kinane","year":"2005","journal-title":"Periodontol 2000"},{"key":"R2-20250130","doi-asserted-by":"crossref","first-page":"3763","DOI":"10.1093\/nar\/19.13.3763","article-title":"Repeat polymorphisms in the interleukin-4 gene (IL4)","volume":"19","author":"Mout","year":"1991","journal-title":"Nucleic Acids Res"},{"key":"R3-20250130","first-page":"5605","article-title":"L-35 may maintain homeostasis of the immune microenvironment in periodontitis","volume":"14","author":"Jin","year":"2017","journal-title":"Exp Ther Med"},{"key":"R4-20250130","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s12903-018-0481-y","article-title":"IL-13 -1112 polymorphism and periodontal disease susceptibility:A meta-analysis","volume":"7","author":"Zhang","year":"2018","journal-title":"BMC Oral Health"},{"key":"R5-20250130","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1001\/jama.279.4.281","article-title":"Effect of the statistical significance of results on the time to completion and publication of randomized efficacy trials","volume":"279","author":"Loannidis","year":"1998","journal-title":"JAMA"},{"key":"R6-20250130","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1902\/jop.2015.140666","article-title":"Level of Interleukin-35 in Gingival Crevicular Fluid, Saliva, and Plasma in Periodontal Disease and Health","volume":"86","author":"K\u00f6seo\u011flu","year":"2015","journal-title":"J Periodontol"},{"key":"R7-20250130","article-title":"Kolte A Levels of Interleukin-35 changes in Gingival Crevicular Fluid after Non-Surgical Periodontal Therapy in stage III periodontitis: a randomized, controlled clinical Trial; 2022, PREPRINT (Version 1) available at Research Square","author":"Jadhav"},{"key":"R8-20250130","article-title":"Gingival Crevicular Fluid Levels of Interleukin-35 in Periodontal Health, Chronic Periodontitis &the Effect of Scaling &Root Planning on Interleukin-35 Levels in Chronic Periodontitis\u2013A Randomized Controlled Clinical Study","volume":"13","author":"Varghese","year":"2021","journal-title":"RGUHS J Dent Sci"},{"key":"R9-20250130","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.sdentj.2023.09.006","article-title":"Estimating the salivary levels of IL-35 in smokers with periodontitis:A cross sectional study","volume":"36","author":"Yuvashri","year":"2024","journal-title":"Saudi Dent J"},{"key":"R10-20250130","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1038\/ni.1952","article-title":"IL-35-mediated induction of a potent regulatory T cell population","volume":"11","author":"Collison","year":"2010","journal-title":"Nat Immunol"},{"key":"R11-20250130","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1111\/j.1600-065X.2008.00704.x","article-title":"Interleukin-35:Odd one out or part of the family?","volume":"226","author":"Collison","year":"2008","journal-title":"Immunol Rev"},{"key":"R12-20250130","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1902\/jop.2000.71.11.1699","article-title":"Evidence of a substantial genetic basis for risk of adult periodontitis","volume":"71","author":"Michalowicz","year":"2000","journal-title":"J Periodontol"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_1483_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:28:58Z","timestamp":1787837338000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_1483_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":12,"journal-issue":{"issue":"Suppl 5","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_1483_24","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"2024-10-10","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-26","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}