{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:31:58Z","timestamp":1786649518567,"version":"build-2736575974"},"reference-count":7,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Alcoholic hepatitis is a chronic liver disease characterized by jaundice and hepatomegaly, influenced by excessive alcohol consumption, elevated pro-inflammatory cytokines, and decreased leptin levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim and Objectives:<\/jats:title>\n                    <jats:p>This study aimed to assess leptin\u2019s role in regulating IL-6 and TNF-\u03b1 in alcoholic hepatitis patients, evaluate serum levels of these hormones, and investigate their interrelationship.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology:<\/jats:title>\n                    <jats:p>A total of 160 venous blood samples were collected, comprising 80 samples from nonalcoholic individuals (controls) and 80 from alcoholic patients (cases). The samples were centrifuged, and serum was collected in Eppendorf tubes. Approximately 1.5\u20132 mL of serum was required to perform enzyme-linked immunosorbent assay (ELISA) tests for leptin, IL-6, and TNF-\u03b1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      In a cohort of 80 alcoholic patients, decreased leptin levels were found in 82.5%, particularly prevalent in the 40\u201350 years age group with alcoholic hepatitis (88.9%). Elevated IL-6 and TNF-\u03b1 levels were each observed in 53.8% of participants, showing significant correlations with reduced leptin (93.0% and 95.3%, respectively;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05). Notably, all patients with elevated bilirubin had both decreased leptin and increased TNF-\u03b1, while among those with normal bilirubin, reduced leptin (71.4%) and TNF-\u03b1 (75.5%) were still common.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The study reveals a significant link between leptin, IL-6, and TNF-\u03b1 levels and liver dysfunction indicators like total bilirubin, indicating leptin\u2019s potential as a biomarker for hepatic inflammation in alcoholic liver disease, emphasizing systemic inflammatory pathways\u2019 role in its pathophysiology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_1486_25","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T17:00:23Z","timestamp":1766422823000},"page":"236-238","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["A Comparative Observational Study on the Effect of Leptin in Regulating Interleukin-6 (IL-6) and Tumor Necrosis Factor-Alpha (TNF-\u03b1) in Patients with Alcoholic Hepatitis in a Tertiary Healthcare Setting"],"prefix":"10.4103","volume":"17","author":[{"given":"C. Sneha","family":"Shankari","sequence":"first","affiliation":[{"name":"Department of Pathology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission\u2019s Research Foundation (Deemed to be University), Puducherry, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jawahar","family":"Ramasamy","sequence":"additional","affiliation":[{"name":"Department of Pathology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission\u2019s Research Foundation (Deemed to be University), Puducherry, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. Vigneswara","family":"Moorthi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission\u2019s Research Foundation (Deemed to be University), Puducherry, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siva","family":"Kaliyamoorthy","sequence":"additional","affiliation":[{"name":"Department of Pathology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission\u2019s Research Foundation (Deemed to be University), Puducherry, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravikumar","family":"Sambandam","sequence":"additional","affiliation":[{"name":"Multi-Disciplinary Centre for Biomedical Research, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission\u2019s Research Foundation (Deemed to be University), Puducherry, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. M.","family":"Karthik","sequence":"additional","affiliation":[{"name":"Department of Medicine, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission\u2019s Research Foundation (Deemed to be University), Puducherry, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"key":"R1-20251223","first-page":"829","article-title":"Role of leptin resistance in the development of obesity in older patients","volume":"8","author":"Carter","year":"2013","journal-title":"Clin Interv Aging"},{"key":"R2-20251223","doi-asserted-by":"crossref","first-page":"2152","DOI":"10.1172\/JCI118653","article-title":"Endotoxin and cytokines induce expression of leptin, the ob gene product, in hamsters","volume":"97","author":"Grunfeld","year":"1996","journal-title":"J Clin Invest"},{"key":"R3-20251223","doi-asserted-by":"crossref","first-page":"117177","DOI":"10.1016\/j.biopha.2024.117177","article-title":"The interplay between cytokines, inflammation, and antioxidants: Mechanistic insights and therapeutic potentials of various antioxidants and anti-cytokine compounds","volume":"178","author":"Bhol","year":"2024","journal-title":"Biomed Pharmacother"},{"key":"R4-20251223","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1093\/abm\/kaab085","article-title":"Daily physical activity, sedentary behavior and alcohol use in at-risk college students","volume":"56","author":"West","year":"2022","journal-title":"Ann Behav Med"},{"key":"R5-20251223","first-page":"352","article-title":"Measurement and clinical usefulness of bilirubin in liver disease","volume":"2","author":"Ruiz","year":"2021","journal-title":"Adv Lab Med"},{"key":"R6-20251223","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/s00281-022-00910-2","article-title":"TNF in the liver: Targeting a central player in inflammation","volume":"44","author":"Tiegs","year":"2022","journal-title":"Semin Immunopathol"},{"key":"R7-20251223","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1111\/apt.15515","article-title":"Raised serum Interleukin-6 identifies patients with liver cirrhosis at high risk for overt hepatic encephalopathy","volume":"50","author":"Labenz","year":"2019","journal-title":"Aliment Pharmacol Ther"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_1486_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T18:54:58Z","timestamp":1786647298000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_1486_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":7,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_1486_25","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-08-23","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-30","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}