{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:30:50Z","timestamp":1787776250729,"version":"build-2784847793"},"reference-count":14,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 1","license":[{"start":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T00:00:00Z","timestamp":1739577600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Infectious diseases are the world\u2019s leading cause of premature mortality, almost killing 65,000 people every day.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      The main objective of the study is to find the extraction and analysis of antimicrobial compounds from\n                      <jats:italic toggle=\"yes\">Onosma Bracteatum<\/jats:italic>\n                      using response surface methodology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      ATCC culture samples and antibiotic discs were purchased from PCSIR and scientific markets. All chemicals with reagents of analytical and commercial grades were purchased from Sigma Millipore Chemical Germany, Daejung Chemicals, China, and Unichem England.\n                      <jats:italic toggle=\"yes\">ONOSMA BRACTEATUM<\/jats:italic>\n                      was purchased from the herbal market and its extracts were prepared in the lab.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The plant extracts; ONO (3.82%) having light green color were arranged and further valued by performing phytochemical analysis of plant extracts. The highest quantity of phenolic content was observed in Ethanolic, Chloroform, and\n                      <jats:italic toggle=\"yes\">n-hexane<\/jats:italic>\n                      extract of fruit with values 97.0 \u00b1 0.91, 39.5 \u00b1 0.55, and 8.1 \u00b1 0.67 \u03bcg GAE\/mg extract respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      It very well might be deduced from this research that in-vitro investigation of the\n                      <jats:italic toggle=\"yes\">O. bracteatum<\/jats:italic>\n                      plant showed a critical zone of inhibition against\n                      <jats:italic toggle=\"yes\">Escherichia coli, Bacillus subtilis, Staph aureus, klebsiella pneumonia<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_1595_24","type":"journal-article","created":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T14:00:06Z","timestamp":1739628006000},"page":"S865-S868","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Extraction and Analysis of Antimicrobial Compounds from Onosma Bracteatum Using Response Surface Methodology"],"prefix":"10.4103","volume":"17","author":[{"given":"Syed Y.","family":"Kazmi","sequence":"first","affiliation":[{"name":"Department of Basic Science, College of Medicine, Majmaah University, Majmaah, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habeeb A.","family":"Baig","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Northern Border University, Arar, Saudi 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