{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:29:54Z","timestamp":1787030994112,"version":"build-2736575974"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives:<\/jats:title>\n                    <jats:p>Cardiac hypertrophy is a maladaptive response to chronic hypertension and is often associated with impaired calcium handling. Andrographolide, a bioactive diterpenoid, has shown cardioprotective effects in several preclinical models, but its role in pressure-overload-induced hypertrophy remains unclear. This study evaluated the effects of andrographolide on cardiac hypertrophy, calcium-handling gene expression, and plasma B-type natriuretic peptide (BNP) levels in a deoxycorticosterone acetate (DOCA)-salt rat model of hypertension.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      Fifteen male Sprague-Dawley rats underwent unilateral nephrectomy followed one week later by DOCA administration (20 mg\/kg, subcutaneous, twice weekly) and 1% NaCl drinking water. After two weeks of DOCA-salt exposure, rats were randomized to receive oral andrographolide (30 mg\/kg\/day in 0.5% carboxymethyl cellulose) or no treatment. Blood pressure was measured at weeks 2 and 5 using a tail-cuff system. At week 5, echocardiography was performed, followed by euthanasia and collection of cardiac tissue and plasma. BNP and cardiac calcium levels were measured by enzyme-linked immunosorbent assay and colorimetric assay, respectively. Sarco\/endoplasmic reticulum Ca\n                      <jats:sup>2<\/jats:sup>\n                      +-ATPase 2a (SERCA2a) and ryanodine receptor (RyR) mRNA expression were quantified by PCR using \u03b2-actin as the reference gene.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>DOCA-salt treatment significantly increased blood pressure, left ventricular hypertrophy indices, plasma BNP, and cardiac calcium levels. Andrographolide attenuated these changes and restored SERCA2a and RyR expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Andrographolide alleviates DOCA-salt-induced cardiac hypertrophy, likely by restoring calcium-handling pathways, supporting its therapeutic potential in hypertension-induced cardiac remodeling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_1700_25","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T22:02:49Z","timestamp":1783720969000},"page":"203-208","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["Andrographolide Mitigates DOCA-Salt-Induced Cardiac Hypertrophy by Regulating Calcium-Handling Genes"],"prefix":"10.4103","volume":"18","author":[{"given":"Muhamad Rizqy","family":"Fadhillah","sequence":"first","affiliation":[{"name":"Doctoral Program in Biomedical Science, Universitas Indonesia, Central Jakarta, Java, Indonesia"},{"name":"Department of Pharmacology and Therapeutics, Universitas Indonesia, Central Jakarta, Java, Indonesia"},{"name":"Bioinformatics Research Center, Indonesian Institute of Bioinformatics (INBIO), Malang, Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wawaimuli","family":"Arozal","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Universitas Indonesia, Central Jakarta, Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond Rubianto","family":"Tjandrawinata","sequence":"additional","affiliation":[{"name":"Center for Pharmaceutical and Nutraceutical Research and Policy, Atma Jaya Catholic University of Indonesia, South Jakarta, Java, 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