{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T20:28:10Z","timestamp":1786739290126,"version":"build-2736575974"},"reference-count":9,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 1","license":[{"start":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T00:00:00Z","timestamp":1744416000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:p>\n                    A 28-year-old female with polycystic ovary syndrome (PCOS) and a history of primary infertility for eight years underwent\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    maturation (IVM) at Wardha. The patient\u2019s clinical profile revealed elevated levels of anti-M\u00fcllerian hormone (AMH) levels, a high luteinizing hormone\/follicle-stimulating hormone (LH\/FSH) ratio, and ultrasound findings consistent with PCOS. After mild ovarian stimulation, 12 immature oocytes were retrieved and cultured in Neurotrophin-4 (NT-4) enriched IVM medium. A total of eight oocytes matured to the metaphase II stage, with seven achieving fertilization via intracytoplasmic sperm injection (ICSI). Out of the fertilized oocytes, five developed into high-quality blastocysts. The patient received an optimized endometrial preparation protocol, including metformin, estradiol, cabergoline, and L-arginine supplementation. Following frozen embryo transfer, pregnancy was confirmed by positive beta-human chorionic gonadotropin (\n                    <jats:italic toggle=\"yes\">\u03b2<\/jats:italic>\n                    -hCG) levels and ultrasound. This study highlights IVM\u2019s potential to mitigate ovarian hyperstimulation syndrome (OHSS) risks while achieving high success rates in PCOS patients. The inclusion of NT-4 in maturation media significantly improved oocyte quality, fertilization rates, and blastocyst development. These findings underscore IVM as a promising assisted reproductive technology (ART) approach, offering enhanced safety and efficacy for fertility treatment in PCOS patients.\n                  <\/jats:p>","DOI":"10.4103\/jpbs.jpbs_207_25","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T08:00:18Z","timestamp":1744617618000},"page":"S1034-S1037","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Optimizing Oocyte Quality in Polycystic Ovary Syndrome (PCOS): The Impact of Neurotrophin-4 in In Vitro Maturation Protocols"],"prefix":"10.4103","volume":"17","author":[{"given":"Ganesh","family":"Kalaskar","sequence":"first","affiliation":[{"name":"Department of Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akash","family":"More","sequence":"additional","affiliation":[{"name":"Department of Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namrata","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Department of Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakhan Parsharam","family":"Padwal","sequence":"additional","affiliation":[{"name":"Department of Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devanshi","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juhi","family":"Barai","sequence":"additional","affiliation":[{"name":"Department of Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aru","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Department of Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,4,12]]},"reference":[{"key":"R1-20250513","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.fertnstert.2023.02.010","article-title":"Oocyte in vitro maturation: Physiological basis and application to clinical practice","volume":"119","author":"Gilchrist","year":"2023","journal-title":"Fertil Steril"},{"key":"R2-20250513","doi-asserted-by":"crossref","first-page":"615957","DOI":"10.3389\/fendo.2020.615957","article-title":"Factors associated with ovarian hyperstimulation syndrome (ohss) severity in women with polycystic ovary syndrome undergoing IVF\/ICSI","volume":"11","author":"Sun","year":"2021","journal-title":"Front Endocrinol"},{"key":"R3-20250513","doi-asserted-by":"crossref","first-page":"70","DOI":"10.4103\/0974-1208.86080","article-title":"Ovarian hyperstimulation syndrome","volume":"4","author":"Kumar","year":"2011","journal-title":"J Hum Reprod 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glial cell line-derived neurotrophic factor in the ovary: Physiological and pathophysiological implications","volume":"25","author":"Chang","year":"2019","journal-title":"Hum Reprod Update"},{"key":"R9-20250513","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nbt1189","article-title":"Neurotrophins mediate human embryonic stem cell survival","volume":"24","author":"Pyle","year":"2006","journal-title":"Nat Biotechnol"},{"key":"R10-20250513","doi-asserted-by":"crossref","first-page":"779298","DOI":"10.3389\/fvets.2021.779298","article-title":"Beneficial effects of neurotrophin-4 supplementation during in vitro maturation of porcine cumulus-oocyte complexes and subsequent embryonic development after parthenogenetic activation","volume":"8","author":"Kim","year":"2021","journal-title":"Front Vet Sci"}],"container-title":["Journal of Pharmacy and Bioallied 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