{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:24:06Z","timestamp":1787775846829,"version":"build-2784847793"},"reference-count":12,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 1","license":[{"start":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T00:00:00Z","timestamp":1744416000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,5]]},"abstract":"\n A\n BSTRACT<\/jats:sc>\n <\/jats:title>\n \n Background:<\/jats:title>\n Infertility is a growing concern, affecting 8%\u201312% of reproductive-age couples worldwide. Male infertility accounts for 20%\u201330% of cases and contributes to 50% of fertility problems. Semen analysis is the traditional method used to assess male fertility, but it may not adequately reflect sperm DNA integrity, which plays a crucial role in successful conception. Increased oxidative stress (OS) and lifestyle factors like obesity, smoking, and alcohol consumption are known to damage sperm DNA, leading to infertility. Sperm DNA fragmentation (SDF) has emerged as a critical parameter for assessing male fertility potential. This study aims to evaluate the impact of lifestyle modifications on sperm DNA fragmentation in men with infertility.<\/jats:p>\n <\/jats:sec>\n \n Objective:<\/jats:title>\n The objectives of this study are to identify male partners exhibiting abnormal semen parameters, with a focus on assessing the impact of lifestyle modifications\u2014such as quitting smoking, reducing alcohol intake, and increasing physical fitness\u2014on sperm DNA fragmentation. Additionally, the study aims to evaluate the role of sperm DNA integrity in the diagnosis of infertility, as sperm DNA fragmentation is increasingly recognized as a critical factor influencing fertility outcomes. By examining these factors, the study seeks to enhance understanding of how lifestyle changes can potentially improve male fertility through the reduction of DNA fragmentation.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n This prospective study will be conducted at Sawangi, Maharashtra, over 6 months. The study will include male patients with sperm abnormalities and high DNA fragmentation enrolled at an IVF center. Semen analysis, DNA fragmentation testing (using the sperm chromatin dispersion test), and a detailed lifestyle questionnaire will be conducted. Participants will receive advice on lifestyle changes, including cessation of smoking and alcohol consumption and encouragement of physical fitness. Post-modification sperm DNA fragmentation levels will be reassessed.<\/jats:p>\n <\/jats:sec>\n \n Expected Results:<\/jats:title>\n A reduction in sperm DNA fragmentation index (DFI) following lifestyle modifications results in improved sperm quality and fertility outcomes.<\/jats:p>\n <\/jats:sec>\n \n Study Implications:<\/jats:title>\n This study will offer valuable insights into the potential benefits of lifestyle changes on male fertility. It will highlight the importance of sperm DNA integrity and provide a diagnostic tool for male infertility.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_259_25","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T08:00:31Z","timestamp":1744617631000},"page":"S901-S904","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Study Protocol: Effect of Lifestyle Modification on Sperm DNA Fragmentation Index"],"prefix":"10.4103","volume":"17","author":[{"given":"Neha","family":"Nawale","sequence":"first","affiliation":[{"name":"Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akash","family":"More","sequence":"additional","affiliation":[{"name":"Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namrata","family":"Anjankar","sequence":"additional","affiliation":[{"name":"Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanket","family":"Mahajan","sequence":"additional","affiliation":[{"name":"Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radha","family":"Bondare","sequence":"additional","affiliation":[{"name":"Clinical Embryology, School of Allied Health Sciences, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, 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on male infertility and its correlation with the expression levels of the PTPRN2 and PGAM5 genes","volume":"14","author":"Amor","year":"2023","journal-title":"Genes (Basel)"},{"key":"R8-20250513","doi-asserted-by":"crossref","first-page":"101","DOI":"10.5653\/cerm.2018.45.3.101","article-title":"What should be done for men with sperm DNA fragmentation?","volume":"45","author":"Kim","year":"2018","journal-title":"Clin Exp Reprod Med"},{"key":"R9-20250513","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/321901","article-title":"Ejaculate oxidative stress is related with sperm DNA fragmentation and round cells","volume":"2015","author":"Iommiello","year":"2015","journal-title":"Int J Endocrinol"},{"key":"R10-20250513","doi-asserted-by":"crossref","first-page":"e15203","DOI":"10.1016\/j.heliyon.2023.e15203","article-title":"Results of lifestyle modification promotion and reproductive\/general health check for male partners of couples seeking conception","volume":"9","author":"Komiya","year":"2023","journal-title":"Heliyon"},{"key":"R11-20250513","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1590\/s1677-5538.ibju.2021.0604","article-title":"The combined effect of lifestyle intervention and antioxidant therapy on sperm DNA fragmentation and seminal oxidative stress in IVF patients: A pilot study","volume":"48","author":"Humaidan","year":"2022","journal-title":"Int Braz J Urol"},{"key":"R12-20250513","doi-asserted-by":"crossref","first-page":"e13874","DOI":"10.1111\/and.13874","article-title":"Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations","volume":"53","author":"Esteves","year":"2021","journal-title":"Andrologia"}],"container-title":["Journal of Pharmacy and Bioallied 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