{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:38:57Z","timestamp":1787841537805,"version":"build-2784847793"},"reference-count":9,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7]]},"abstract":"\n A\n BSTRACT<\/jats:sc>\n <\/jats:title>\n \n Background:<\/jats:title>\n Antibiotic resistance in common pathogenic bacteria is linked with the genetic makeup. The genetic basis of antibiotic resistance may vary in different species or pathophysiological conditions.<\/jats:p>\n <\/jats:sec>\n \n Objectives:<\/jats:title>\n \n We studied the antibiotic resistance in\n Klebsiella pneumonia<\/jats:italic>\n isolates from DFU in the western Indian population. We also studied the presence of ESBL and MBL mechanisms of antibiotic resistance along with the prevalence of the genes involved in ESBL (TEM\n ESBL<\/jats:italic>\n , SHV\n ESBL<\/jats:italic>\n , and CTX-M\n ESBL<\/jats:italic>\n ) and MBL (NDM-1\n bla<\/jats:italic>\n , KPC\n bla<\/jats:italic>\n , OXA-48\n bla<\/jats:italic>\n , and VIM\n bla<\/jats:italic>\n ) production.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n A total of 161\n K. pneumoniae<\/jats:italic>\n isolates were analyzed; among which 50.93% were positive for ESBL and 45.96% were positive for MBL production. Most of the isolates were resistant to antibiotics used in the present study and partially resistant to Imipenem and Amikacin. There was no relation between the antibiotic resistance of the isolates and the production of ESBL or MBL mechanism of antibiotic resistance. Further, TEM\n ESBL<\/jats:italic>\n was the most prevalent gene in\n K. pneumoniae<\/jats:italic>\n isolates followed by CTX-M\n ESBL<\/jats:italic>\n , NDM-1\n bla<\/jats:italic>\n , SHV\n ESBL<\/jats:italic>\n , and KPC\n bla<\/jats:italic>\n . VIM\n bla<\/jats:italic>\n was the least prevalent gene found in\n K. pneumoniae<\/jats:italic>\n isolates. There was no difference in the prevalence of the genes with respect to the presence or absence of ESBL and MBL mechanism of resistance. Further, there was no relation between the prevalence of the genes and antibiotic resistance in\n K. pneumoniae<\/jats:italic>\n isolates.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n These results along with the literature review suggest that the prevalence of the genes involved in antibiotic resistance mechanisms are widespread in India and their distribution varies in different studies.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_349_24","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T02:03:25Z","timestamp":1722564205000},"page":"S2524-S2526","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Characterization of Extended-Spectrum Beta-Lactamase (ESBL) and Metallo-Beta-Lactamase (MBL) Producing Klebsiella pneumoniae from Diabetic Foot Ulcer (DFU)"],"prefix":"10.4103","volume":"16","author":[{"given":"Dipak S.","family":"Kale","sequence":"first","affiliation":[{"name":"Department of Microbiology, Krishna Vishwa Vidyapeeth \u201cDeemed to be University\u201d, Dist-Satara, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geeta S.","family":"Karande","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Krishna Vishwa Vidyapeeth \u201cDeemed to be University\u201d, Dist-Satara, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalpana D.","family":"Kale","sequence":"additional","affiliation":[{"name":"Department of Pathology, Kale Pathology Laboratory, Dist-Satara, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish R.","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Krishna Vishwa Vidyapeeth \u201cDeemed to be University\u201d, Dist-Satara, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailas D.","family":"Datkhile","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Krishna Vishwa Vidyapeeth, \u201cDeemed to be University\u201d, Karad, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,7,31]]},"reference":[{"key":"R1-20240829","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1089\/fpd.2020.2847","article-title":"Klebsiella pneumoniae: Prevalence, reservoirs, antimicrobial resistance, pathogenicity, and infection: A hitherto unrecognized zoonotic bacterium","volume":"18","author":"Hu","year":"2021","journal-title":"Foodborne Pathogens Dis"},{"key":"R2-20240829","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.2217\/fmb.14.48","article-title":"Molecular pathogenesis of Klebsiella pneumoniae","volume":"9","author":"Li","year":"2014","journal-title":"Future Microbiol"},{"key":"R3-20240829","doi-asserted-by":"crossref","first-page":"483","DOI":"10.3389\/fcimb.2017.00483","article-title":"Antimicrobial resistance of hypervirulent Klebsiella pneumoniae: Epidemiology, hypervirulence-associated determinants, and resistance mechanisms","volume":"7","author":"Lee","year":"2017","journal-title":"Front Cell Infect Microbiol"},{"key":"R4-20240829","doi-asserted-by":"crossref","first-page":"6278","DOI":"10.3390\/ijerph17176278","article-title":"The characteristic of virulence, biofilm and antibiotic resistance of Klebsiella pneumoniae","volume":"17","author":"Wang","year":"2020","journal-title":"Int J Environ Res Public Health"},{"issue":"(Suppl_1)","key":"R5-20240829","doi-asserted-by":"crossref","first-page":"S18","DOI":"10.1093\/infdis\/jiw378","article-title":"Clinical implications of genomic adaptation and evolution of carbapenem-resistant Klebsiella pneumoniae","volume":"215","author":"Gomez-Simmonds","year":"2017","journal-title":"J Infect Dis"},{"key":"R6-20240829","doi-asserted-by":"crossref","first-page":"105040","DOI":"10.1016\/j.micpath.2021.105040","article-title":"Genetic basis of molecular mechanisms in ?-lactam resistant gram-negative bacteria","volume":"158","author":"Hussain","year":"2021","journal-title":"Microb Pathog"},{"key":"R7-20240829","doi-asserted-by":"crossref","first-page":"1830","DOI":"10.3389\/fmicb.2016.01830","article-title":"High prevalence of ESBL-producing Klebsiella pneumoniae causing community-onset infections in China","volume":"7","author":"Zhang","year":"2016","journal-title":"Front Microbiol"},{"key":"R8-20240829","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/nrmicro1614","article-title":"The antibiotic resistome: The nexus of chemical and genetic diversity","volume":"5","author":"Wright","year":"2007","journal-title":"Nat Rev Microbiol"},{"key":"R9-20240829","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1046\/j.1524-475X.2002.t01-1-10602.x","article-title":"Potential role of anaerobic cocci in impaired human wound healing","volume":"10","author":"Wall","year":"2002","journal-title":"Wound Repair Regen"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_349_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:59:49Z","timestamp":1787839189000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_349_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":9,"journal-issue":{"issue":"Suppl 3","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_349_24","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"2024-04-02","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-09","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}