{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:38:59Z","timestamp":1787841539678,"version":"build-2784847793"},"reference-count":7,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 3","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,7]]},"abstract":"\n A\n BSTRACT<\/jats:sc>\n <\/jats:title>\n \n Background:<\/jats:title>\n \n Severe Early Childhood Caries (S-ECC) is a prevalent oral health issue among young children, often associated with specific caries-associated bacteria. This\n in vitro<\/jats:italic>\n study investigates the impact of diet on the growth and activity of caries-associated bacteria in S-ECC.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Plaque samples of\n Streptococcus mutans<\/jats:italic>\n and\n Lactobacillus<\/jats:italic>\n species were procured from laboratory. The samples were exposed to different dietary conditions, including high-sugar, high-carbohydrate, and control diets. The growth kinetics of caries-associated bacteria, including\n S. mutans<\/jats:italic>\n and\n Lactobacillus<\/jats:italic>\n species, were monitored using quantitative polymerase chain reaction (qPCR) and colony-forming unit (CFU) assays.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Under high-sugar conditions, the growth of\n S. mutans<\/jats:italic>\n increased by 50% compared to the control group, as evidenced by qPCR analysis.\n Lactobacillus<\/jats:italic>\n species also exhibited a significant increase in CFU counts in response to high-carbohydrate diets, showing a 40% rise compared to controls.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n This\n in vitro<\/jats:italic>\n study highlights the influence of diet on the proliferation of caries-associated bacteria in severe early childhood caries. High-sugar and high-carbohydrate diets contribute to the growth and activity of\n S. mutans<\/jats:italic>\n and\n Lactobacillus<\/jats:italic>\n species, exacerbating the progression of dental caries in affected children.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_363_24","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T02:27:31Z","timestamp":1722565651000},"page":"S2664-S2666","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Diet and Caries-associated Bacteria in Severe Early Childhood Caries An In vitro Study"],"prefix":"10.4103","volume":"16","author":[{"given":"Raghavendra","family":"Pidamale","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, KVG Dental College and Hospital, Kurunjibhag, Sullia, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prem S.","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Preventive Dentistry, Sharda Dental Clinic, Plot No 58\/1. O.u.t. Colony Sainikpuri, Secunderabad, Telengana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghavendr","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pediatric and Preventive Dentistry, Purvanchal Institute of Dental Sciences, GIDA, Gorakhpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MD","family":"Imran","sequence":"additional","affiliation":[{"name":"Department of Paediatric and Preventive Dentistry, Patna Medical College and Hospital, Ashok Raj Path, Patna, Bihar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roopan","family":"Prakash","sequence":"additional","affiliation":[{"name":"Oral Patholgy and Microbiology, College of Dental Sciences Amargadh, Bhavnagar, Gujarat, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasthuripriya","family":"Kuppusamy","sequence":"additional","affiliation":[{"name":"Public Health Dentistry, Indira Gandhi Institute of Dental Sciences, Puducherry, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,7,18]]},"reference":[{"key":"R1-20240829","first-page":"52","article-title":"Policy on early childhood caries (ECC): Classifications, consequences, and preventive strategies","volume":"38","year":"2016","journal-title":"Pediatr Dent"},{"key":"R2-20240829","first-page":"106","article-title":"Mutans streptococci: Acquisition and transmission","volume":"28","author":"Berkowitz","year":"2006","journal-title":"Pediatr Dent"},{"key":"R3-20240829","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1177\/0022034510379602","article-title":"The role of bacteria in the caries process: ecological perspectives","volume":"90","author":"Takahashi","year":"2011","journal-title":"J Dent Res"},{"key":"R4-20240829","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.cden.2010.03.002","article-title":"Microbiology of dental plaque biofilms and their role in oral health and caries","volume":"54","author":"Marsh","year":"2010","journal-title":"Dent Clin North Am"},{"key":"R5-20240829","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1079\/PHN2003589","article-title":"Diet, nutrition and the prevention of dental diseases","volume":"7","author":"Moynihan","year":"2004","journal-title":"Public Health Nutr"},{"key":"R6-20240829","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1111\/j.1834-7819.2008.00064.x","article-title":"Dental caries: A dynamic disease process","volume":"53","author":"Featherstone","year":"2008","journal-title":"Aust Dent J"},{"issue":"Suppl 2","key":"R7-20240829","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1111\/idj.12072","article-title":"Diet and the microbial aetiology of dental caries: New paradigms","volume":"63","author":"Bradshaw","year":"2013","journal-title":"Int Dent J"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_363_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:59:49Z","timestamp":1787839189000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_363_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":7,"journal-issue":{"issue":"Suppl 3","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_363_24","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"2024-04-05","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-15","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}