{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T19:46:17Z","timestamp":1790451977780,"version":"4.1.0"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>To evaluate correlation of PRF and HAS with glycemic control (HbA1c) and other systemic parameters amongst the chronic periodontitis patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>Twenty-seven non-smoker, non-tobacco users, non-alcoholic moderate chronic periodontitis volunteers (Stage II, Grade A or B), aging between 40 and 50 years of both genders with type 2 diabetes mellitus (T2DM, HbA1c &gt;6.5%) or without T2DM (HbA1c &lt;6.5%), were recruited. After recording vitals and demographic parameters, 20 ml of intravenous fasting blood was collected, of which 11 ml blood was used for investigating complete blood count, lipid profile, glycated hemoglobin (HbA1c), C-reactive protein, and fasting insulin level. As per the standard protocol, PRF and HAS was prepared from the 9-ml blood sample of the patients. PRF obtained was quantified using Wensar\u2019s analytical balance with a readability of 0.0001 g. Prepared HAS from the fibrin clot was quantified using a micropipette and stored in 2 ml Eppendorf vials.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Mean values of PRF and HAS obtained in the study were 2.0089 \u00b1 0.844 g (ranged between 0.0 g and 3.968 g) and 1.540 \u00b1 0.552 ml (ranged between 0.00 and 3.500 ml), respectively. The amount of PRF and HAS obtained were significantly higher in non-diabetic patients (mean 2.3093 g and 1.743 ml, respectively) as compared to diabetic patients (mean 1.5762 g and 1.266 ml, respectively). Results suggested a significant correlation between HbA1c level and quantification of PRF (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.007) and HAS (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.037), whereas no correlation was observed between HbA1c levels and hematologic, lipid, and demographic parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Within the limitation of the present study, it can be concluded that a significant correlation exists between the HbA1c level and quantification of PRF and HAS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_428_22","type":"journal-article","created":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T02:07:24Z","timestamp":1689127644000},"page":"S601-S607","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":6,"title":["Quantitative Association of Platelet-Rich Fibrin (PRF) and Hyperacute Serum (HAS) with Glycemic Control (HbA1c) in Chronic Periodontitis Patients: An Ex vivo Study"],"prefix":"10.4103","volume":"15","author":[{"given":"Vivek Kumar","family":"Bains","sequence":"first","affiliation":[{"name":"Department of Periodontology, Saraswati Dental College and Hospital, Lucknow, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaideep","family":"Mahendra","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Little","family":"Mahendra","sequence":"additional","affiliation":[{"name":"Maktoum Bin Hamdan Dental University College, Dubai, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madhukar","family":"Mittal","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism, AIIMS, Jodhpur, Rajasthan, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valli","family":"Gunam","sequence":"additional","affiliation":[{"name":"College of Allied Health Sciences, Sri Venkateswaraa University, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,7,5]]},"reference":[{"key":"R1-20240829","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1834-7819.2012.01686.x","article-title":"Platelet-rich fibrin increases cell attachment, proliferation and collagen-related protein expression of human osteoblasts","volume":"57","author":"Wu","year":"2012","journal-title":"Aust Dent J"},{"key":"R2-20240829","first-page":"6","article-title":"Comparative evaluation of platelet concentration and its distribution in the buffy coat region of platelet rich fibrin in non-diabetic and controlled diabetic patients:A light microscopic study","volume":"3","author":"Hemalata","year":"2016","journal-title":"Int J Adv Health Sci"},{"key":"R3-20240829","doi-asserted-by":"crossref","first-page":"4854619","DOI":"10.1155\/2018\/4854619","article-title":"The effects of hyperacute serum on the elements of the human subchondral bone marrow niche","volume":"2018","author":"Simon","year":"2018","journal-title":"Stem Cells Int"},{"key":"R4-20240829","first-page":"2","article-title":"Periodontal disease among diabetics in Iraq","volume":"7","author":"Mansour","year":"2005","journal-title":"MedGenMed"},{"key":"R5-20240829","first-page":"668","article-title":"Asurvey on the periodontal status in type 2 diabetic patients","volume":"44","author":"Zhang","year":"2009","journal-title":"Zhonghua Kou Qiang Yi Xue Za Zhi"},{"key":"R6-20240829","doi-asserted-by":"crossref","first-page":"349","DOI":"10.4103\/ccd.ccd_652_18","article-title":"Prevalence of periodontal disease in type 2 diabetes mellitus patients:A cross-sectional study","volume":"10","author":"Singh","year":"2019","journal-title":"Contemp Clin Dent"},{"key":"R7-20240829","doi-asserted-by":"crossref","first-page":"6450742","DOI":"10.1155\/2020\/6450742","article-title":"Association of glycated proteins with inflammatory proteins and periodontal disease parameters","volume":"2020","author":"Panezai","year":"2020","journal-title":"J Diabetes Res"},{"key":"R8-20240829","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1007\/s00784-018-2673-x","article-title":"The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes","volume":"23","author":"Miron","year":"2019","journal-title":"Clin Oral Investig"},{"key":"R9-20240829","doi-asserted-by":"crossref","first-page":"2620","DOI":"10.14260\/jemds\/2020\/570","article-title":"In-Vitro quantitative comparison of platelet rich fibrin, and plasma rich in growth factor, with age, gender, blood parameters","volume":"9","author":"Narayan","year":"2020","journal-title":"J Evol Med Dent Sci"},{"issue":"Suppl 1","key":"R10-20240829","first-page":"S173","article-title":"Periodontitis:Consensus report of Workgroup 2 of the 2017 World workshop on the classification of periodontal and peri-implant diseases and conditions","volume":"89","author":"Papapanou","year":"2018","journal-title":"J Periodontol"},{"key":"R11-20240829","doi-asserted-by":"crossref","first-page":"e81190","DOI":"10.1371\/journal.pone.0081190","article-title":"Serum concentrations of fibroblast growth factors 19 and 21 in women with gestational diabetes mellitus:Association with insulin resistance, adiponectin, and polycystic ovary syndrome history","volume":"8","author":"Wang","year":"2013","journal-title":"PLoS One"},{"key":"R12-20240829","doi-asserted-by":"crossref","first-page":"5163245","DOI":"10.1155\/2019\/5163245","article-title":"Serum fibroblast growth factor 21 levels are positively associated with metabolic syndrome in patients with type 2 diabetes","volume":"2019","author":"Gao","year":"2019","journal-title":"Int J Endocrinol"},{"key":"R13-20240829","first-page":"55","article-title":"Une opportunit\u00e9en paro-implantologie:le PRF","volume":"42","author":"Choukroun","year":"2001","journal-title":"Implantodontie"},{"key":"R14-20240829","doi-asserted-by":"crossref","first-page":"100","DOI":"10.4103\/1305-7456.149653","article-title":"Evaluation of intrabony defects treated with platelet-rich-fibrin or autogenous bone graft:A comparative analysis","volume":"9","author":"Mathur","year":"2015","journal-title":"Eur J Dent"},{"key":"R15-20240829","doi-asserted-by":"crossref","first-page":"264","DOI":"10.4103\/1305-7456.178298","article-title":"Socket preservation by beta-tri-calcium phosphate with collagen compared to platelet-rich fibrin:A clinico-radiographic study","volume":"10","author":"Das","year":"2016","journal-title":"Eur J Dent"},{"key":"R16-20240829","doi-asserted-by":"crossref","first-page":"121","DOI":"10.4103\/1305-7456.175686","article-title":"Patient-centred evaluation of microsurgical management of gingival recession using coronally advanced flap with platelet-rich fibrin or amnion membrane:A comparative analysis","volume":"10","author":"Agarwal","year":"2016","journal-title":"Eur J Dent"},{"key":"R17-20240829","doi-asserted-by":"crossref","first-page":"025002","DOI":"10.1088\/1748-605X\/aa8fa3","article-title":"Evaluation of peri-implant tissues around nanopore surface implants with or without platelet rich fibrin:A clinico-radiographic study","volume":"13","author":"Khan","year":"2018","journal-title":"Biomed Mater"},{"key":"R18-20240829","doi-asserted-by":"crossref","first-page":"158","DOI":"10.5051\/jpis.2014.44.4.158","article-title":"Impact of scaling and root planing on C-reactive protein levels in gingival crevicular fluid and serum in chronic periodontitis patients with or without diabetes mellitus","volume":"44","author":"Mohan","year":"2014","journal-title":"J Periodontal Implant Sci"},{"key":"R20-20240829","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0140-6736(03)15268-3","article-title":"Appropriate body-mass index for Asian populations and its implications for policy and intervention strategies","volume":"363","year":"2004","journal-title":"Lancet"},{"key":"R21-20240829","first-page":"298","article-title":"Factors affecting the preparation, constituents, and clinical efficacy of leukocyte- and platelet- rich fibrin (L-PRF)","volume":"71","author":"Peck","year":"2016","journal-title":"SADJ J South Afr Dent Assoc"},{"key":"R22-20240829","article-title":"Influence of periodontitis and diabetes on structure and cytokine content of platelet-rich fibrin","author":"Gupta","year":"2022","journal-title":"Oral Dis"},{"key":"R23-20240829","doi-asserted-by":"crossref","first-page":"151","DOI":"10.4103\/0972-124X.176390","article-title":"Fibrin network pattern changes of platelet-rich fibrin in young vs old age group of individuals:A cell block cytology study","volume":"20","author":"Yajamanya","year":"2016","journal-title":"J Indian Soc Periodontol"},{"issue":"Suppl","key":"R24-20240829","doi-asserted-by":"crossref","first-page":"26","DOI":"10.14219\/jada.archive.2006.0404","article-title":"Periodontal disease and diabetes:A two-way street","volume":"137","author":"Mealey","year":"2006","journal-title":"J Am Dent Assoc"},{"key":"R25-20240829","doi-asserted-by":"crossref","first-page":"65","DOI":"10.5005\/jp-journals-10015-1693","article-title":"Modification to the centrifugation protocol increases the competence of platelet-rich fibrin in controlled diabetic patients?A Light microscopic study","volume":"11","author":"Abooj","year":"2020","journal-title":"World J Dent"},{"key":"R26-20240829","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1080\/09537104.2017.1293812","article-title":"The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane","volume":"29","author":"Dohan Ehrenfest","year":"2018","journal-title":"Platelets"},{"key":"R27-20240829","first-page":"15347346211052811","article-title":"The role of leukocyte-platelet-rich fibrin in promoting wound healing in diabetic foot ulcers","author":"Wang","year":"2021","journal-title":"Int J Low Extrem Wounds"},{"key":"R28-20240829","first-page":"268","article-title":"Wound Healing in diabetic foot ulcer patients using combined use of platelet rich fibrin and hyaluronic acid, platelet rich fibrin and placebo:An open label, randomized controlled trial","volume":"53","author":"Kartika","year":"2021","journal-title":"Acta Med Indones"},{"key":"R29-20240829","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3390\/ph13040060","article-title":"The treatment of impaired wound healing in diabetes:Looking among old drugs","volume":"13","author":"Spampinato","year":"2020","journal-title":"Pharmaceuticals (Basel)"},{"key":"R30-20240829","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1172\/JCI32169","article-title":"Cellular and molecular basis of wound healing in diabetes","volume":"117","author":"Brem","year":"2007","journal-title":"J Clin Invest"},{"key":"R31-20240829","first-page":"245","article-title":"The effects of photobiomodulation on leukocyte and platelet-rich fibrin as barrier membrane on bone regeneration:An experimental animal study","volume":"39","author":"Surmeli Baran","year":"2021","journal-title":"Photobiomodul Photomed Laser Surg"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_428_22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:12:07Z","timestamp":1787760727000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_428_22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7]]},"references-count":30,"journal-issue":{"issue":"Suppl 1","published-print":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_428_22","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7]]},"assertion":[{"value":"2022-09-30","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-05","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}