{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T02:52:36Z","timestamp":1788317556502,"version":"build-2803163510"},"reference-count":10,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,2]]},"abstract":"\n A\n BSTRACT<\/jats:sc>\n <\/jats:title>\n \n Background:<\/jats:title>\n Oral submucous fibrosis (OSMF) is a recognized potentially malignant oral condition linked to the consumption of areca nut. Chewing areca nut has been shown to elevate soluble copper levels in mouth fluids.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n Participants: The study included a panel of 30 patients with OSMF from Rama Dental College, Kanpur, India, and 30 nonareca chewing individuals serving as controls. Tissue Sample Collection and Analysis: Buccal mucosal biopsies were obtained from both OSMF patients and controls. The tissue copper concentrations were quantified using mass absorption spectrometry (MAS). Additionally, energy-dispersive X-ray microanalysis (EDX) was employed to identify the presence and distribution of copper in the tissue.<\/jats:p>\n <\/jats:sec>\n \n Statistical Analysis:<\/jats:title>\n \n Statistical comparisons were performed using appropriate methods, with a\n P-<\/jats:italic>\n value of less than 0.05 considered statistically significant.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n MAS analysis revealed that the mean tissue copper level was 6.2 \u00b1 3.1 micrograms per gram (\u03bcg\/g) in OSMF specimens (\n n<\/jats:italic>\n = 30), slightly higher than the 4.5 \u00b1 2.0 \u03bcg\/g in the nonareca chewing controls (\n n<\/jats:italic>\n = 30) (\n P<\/jats:italic>\n = 0.1). EDX analysis showed distinct copper peaks in both the epithelium (22\/23) and connective tissue (18\/23) of OSMF specimens compared to control biopsies. These findings were corroborated by secondary ion mass spectrometry (SIMS) in a subset of samples.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n The study revealed higher copper concentrations in buccal mucosal tissue of OSMF patients from Rama Dental College, Kanpur, suggesting a potential connection between copper and the initiation of OSMF.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_904_23","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T15:03:42Z","timestamp":1709219022000},"page":"S629-S630","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":3,"title":["Comparative Analysis of Tissue Copper Levels in Oral Submucous Fibrosis (OSMF) Patients"],"prefix":"10.4103","volume":"16","author":[{"given":"Rahul","family":"Srivastava","sequence":"first","affiliation":[{"name":"Department of Oral medicine and Radiology, Rama Dental College Hospital and Research Center, Kanpur, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhirendra K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Kalinga Institute of Dental Sciences, KIIT Deemed to be University, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallavi A.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Public Health Dentistry, Tagore Dental College, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadi","family":"Raza","sequence":"additional","affiliation":[{"name":"Department of Public Health Dentistry, Mithila Minority Dental College and Hospital, Darbhanga, Bihar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. C.","family":"Sangamesh","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Radiology, Kalinga Institute of Dental Sciences, KIIT DU, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroj S.","family":"Bagde","sequence":"additional","affiliation":[{"name":"Professor, Department of Periodontology, CDCRI, Rajnandgaon, Chhattisgarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"key":"R1-20240829","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1016\/0030-4220(66)90367-7","article-title":"Oral submucous fibrosis","volume":"22","author":"Pindborg","year":"1966","journal-title":"Oral Surg Oral Med Oral Pathol"},{"key":"R2-20240829","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1080\/13556210020091437","article-title":"Global epidemiology of areca nut usage","volume":"7","author":"Gupta","year":"2002","journal-title":"Addict Biol"},{"key":"R3-20240829","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.1600-0714.2006.00485.x","article-title":"Oral submucous fibrosis: Study of 1000 cases from central India","volume":"36","author":"Hazarey","year":"2007","journal-title":"J Oral Pathol Med"},{"key":"R4-20240829","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3945\/an.110.000208","article-title":"Impact of copper limitation on expression and function of multicopper oxidases (ferroxidases)","volume":"2","author":"Prohaska","year":"2011","journal-title":"Adv Nutr"},{"key":"R5-20240829","first-page":"774","article-title":"Serum copper, iron, and ceruloplasmin levels in oral submucous fibrosis and oral squamous cell carcinoma: A preliminary study","volume":"114","author":"Upadhyaya","year":"2012","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol"},{"key":"R6-20240829","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1002\/jcb.10413","article-title":"Lysyl oxidase: Properties, specificity, and biological roles inside and outside of the cell","volume":"88","author":"Kagan","year":"2003","journal-title":"J Cell Biochem"},{"key":"R7-20240829","doi-asserted-by":"crossref","first-page":"837","DOI":"10.2307\/2531595","article-title":"Comparing the areas under two or more correlated receiver operating characteristic curves: A nonparametric approach","volume":"44","author":"DeLong","year":"1988","journal-title":"Biometrics"},{"key":"R8-20240829","first-page":"794","article-title":"Prevalence of oral submucous fibrosis among rural and urban population of Morbi city: A cross-sectional study","volume":"29","author":"Gohel","year":"2018","journal-title":"Indian J Dent Res"},{"key":"R9-20240829","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1001\/jama.2013.281053","article-title":"World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects","volume":"310","year":"2013","journal-title":"JAMA"},{"key":"R10-20240829","first-page":"413","article-title":"Localized expression of lysyl oxidase-like and matrix metalloproteinase 14 in the developing basement membrane of the amphibian, Xenopus laevis: Implications for basement membrane remodeling during metamorphosis","volume":"235","author":"Ishizuya-Oka","year":"2006","journal-title":"Dev Dyn"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_904_23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:22:34Z","timestamp":1787768554000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_904_23"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":10,"journal-issue":{"issue":"Suppl 1","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_904_23","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-09-15","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-23","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}