{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:20:55Z","timestamp":1787840455744,"version":"build-2784847793"},"reference-count":8,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"Suppl 3","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>\n                    A\n                    <jats:sc>BSTRACT<\/jats:sc>\n                  <\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>This study aimed to identify Gram-negative uropathogens (GN-UP)-producing extended-spectrum beta-lactamases (ESBL), metallo-beta-lactamases (MBL), and AmpC enzymes in diabetic patients and to analyze their antibiotic resistance profiles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>Urine samples were cultured, and isolates were tested using the Kirby-Bauer disc diffusion method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      (43%) was the most common isolate, followed by Klebsiella spp. (25%). Among GN-UP, 13.26% were ESBL producers, 63.26% MBL producers, and 17.34% AmpC producers. MBL-producing isolates showed lower resistance to fosfomycin (22.58%), tigecycline (32.25%), and nitrofurantoin (33.87%). The high prevalence of MBL, ESBL, and AmpC producers in diabetic urinary tract infection (UTI) patients complicates effective antibiotic therapy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>These findings underscore the need for regular antimicrobial surveillance and tailored treatment strategies in this vulnerable group.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jpbs.jpbs_914_25","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T12:00:11Z","timestamp":1755777611000},"page":"S2569-S2571","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Gram-Negative Uropathogens in Diabetic Patients: A Study of Ampicillinase Cephalosporinase, Extended-Spectrum Beta-Lactamases, and Metallo Beta-Lactamases Producing Phenotypes"],"prefix":"10.4103","volume":"17","author":[{"given":"Satish R","family":"Patil","sequence":"first","affiliation":[{"name":"Department of Microbiology, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth \u201cDeemed to be University\u201d, Satara, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. S.","family":"Karande","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth \u201cDeemed to be University\u201d, Satara, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindra V.","family":"Shinde","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth \u201cDeemed to be University\u201d, Satara, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satyajeet K.","family":"Pawar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth \u201cDeemed to be University\u201d, Satara, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"R1-20250930","doi-asserted-by":"crossref","first-page":"5474388","DOI":"10.1155\/2022\/5474388","article-title":"Detection of beta-lactamases (ESBL and MBL) producing gram-negative pathogens in national public health laboratory of Nepal","volume":"2022","author":"Shrestha","year":"2022","journal-title":"Int J Microbiol"},{"key":"R2-20250930","first-page":"546","article-title":"Prevalence of extended-spectrum beta lactamase and AmpC producing Enterobacteriaceae among diabetic patients in Bauchi State, Nigeria","volume":"13","author":"Yakubu","year":"2022","journal-title":"Bayero J Pure Appl Sci"},{"key":"R3-20250930","first-page":"1433","article-title":"Prevalence of uropathogens in diabetic patients and their resistance pattern at a tertiary care centre in south India","volume":"3","author":"Ramana","year":"2012","journal-title":"Int J Biol Med Res"},{"key":"R4-20250930","first-page":"29","article-title":"Prevalence of uropathogens in diabetic patients and their susceptibility pattern at a tertiary care center in Nepal-a retrospective study","volume":"3","author":"Kumar","year":"2014","journal-title":"Int J Bio Lab Sci"},{"key":"R5-20250930","doi-asserted-by":"crossref","first-page":"280","DOI":"10.4103\/ijph.IJPH_1413_20","article-title":"Prevalence of uropathogens and their antimicrobial resistance pattern among adult diabetic patients","volume":"65","author":"Kande","year":"2021","journal-title":"Indian J Public Health"},{"key":"R6-20250930","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13104-017-2784-5","article-title":"AmpC and extended spectrum beta-lactamases production among urinary isolates from a tertiary care hospital in Lalitpur, Nepal","volume":"10","author":"Rai","year":"2017","journal-title":"BMC Res Notes"},{"key":"R7-20250930","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.20546\/ijcmas.2017.611.191","article-title":"Detection of ESBLs, MBLs and AmpCs in gram negative uropathogens in tertiary care hospital","volume":"6","author":"Amatullah","year":"2017","journal-title":"Int J Curr Microbiol App Sci"},{"key":"R8-20250930","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1309\/LMHEP4VQHEY2POOK","article-title":"Prevalence and antibiotic susceptibility patterns of extended-spectrum \u00df-lactamase and metallo-\u00df-lactamase\u2013producing uropathogenic escherichia coli isolates","volume":"45","author":"Ghadiri","year":"2014","journal-title":"Lab Med"}],"container-title":["Journal of Pharmacy and Bioallied Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_914_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:23:13Z","timestamp":1787836993000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jpbs.jpbs_914_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,21]]},"references-count":8,"journal-issue":{"issue":"Suppl 3","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.4103\/jpbs.jpbs_914_25","relation":{},"ISSN":["0976-4879","0975-7406"],"issn-type":[{"value":"0976-4879","type":"print"},{"value":"0975-7406","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,21]]},"assertion":[{"value":"2025-05-13","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-11","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}