{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:33:23Z","timestamp":1787776403528,"version":"build-2784847793"},"reference-count":65,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T00:00:00Z","timestamp":1728086400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background &amp; objectives:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                      is a major vector responsible for spreading dengue, chikungunya, yellow fever, and Zika viruses worldwide. These illnesses have increased globally due to climate and environmental changes. Vector control and management are the principal tactics for combating mosquitoes-borne diseases in the absence of an effective vaccine. The study aimed to ascertain bioactivities of\n                      <jats:italic toggle=\"yes\">Ageratum houstonianum<\/jats:italic>\n                      leaf acetone extract (\n                      <jats:italic toggle=\"yes\">Ah<\/jats:italic>\n                      LAE) against\n                      <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Bioefficacy of\n                      <jats:italic toggle=\"yes\">Ah<\/jats:italic>\n                      LAE was tested against fourth instar larvae (L4) of\n                      <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                      using standard WHO protocol. The mortality, growth, and development of larvae, pupae and adults were recorded after exposure to the extract.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic toggle=\"yes\">Ah<\/jats:italic>\n                      LAE showed larvicidal activity against L4 with LC\n                      <jats:sub>50<\/jats:sub>\n                      and LC\n                      <jats:sub>90<\/jats:sub>\n                      values of 401.88 and 691.24 mg\/L, respectively. The mortality of the larvae further increased on subsequent days. The\n                      <jats:italic toggle=\"yes\">Ah<\/jats:italic>\n                      LAE caused a significant reduction in pupae formation and adult emergence. It also increased the larval duration of L4 and development duration of the pupa formed from the treated L4, indicating its growth-suppressing effects. The impact of the\n                      <jats:italic toggle=\"yes\">Ah<\/jats:italic>\n                      LAE was dose-dependent; high concentrations caused reduction in survival and growth of L4. Consequently, the L4-pupal and L4-adult growth indexes decreased. Additionally, the\n                      <jats:italic toggle=\"yes\">Ah<\/jats:italic>\n                      LAE induced developmental anomalies in the form of larva-pupa (L-P) intermediates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation &amp; conclusion:<\/jats:title>\n                    <jats:p>\n                      The study found that the\n                      <jats:italic toggle=\"yes\">Ah<\/jats:italic>\n                      LAE exhibited larvicidal, growth-suppressing, and development-altering activities against\n                      <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                      . The findings suggest the potential of AhALE as a natural insecticidal agent for controlling mosquitoes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/jvbd.jvbd_100_24","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T08:00:41Z","timestamp":1728288041000},"page":"67-77","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of larvicidal, growth-suppressing, and development-altering bioefficacy of Ageratum houstonianum against Aedes aegypti (L.)"],"prefix":"10.4103","volume":"62","author":[{"given":"Vijay Kumar","family":"Shah","sequence":"first","affiliation":[{"name":"Insect Reproduction Laboratory, Department of Zoology, Deshbandhu College, University of Delhi, Kalkaji, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal Kumar","family":"Gupta","sequence":"additional","affiliation":[{"name":"Insect 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