{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:36:10Z","timestamp":1787070970990,"version":"build-2736575974"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"\n Background & objectives:<\/jats:title>\n \n Drug-resistant strains of\n Plasmodium falciparum<\/jats:italic>\n pose a major challenge to malaria control and elimination. The present study focuses on the GTP-cyclohydrolase 1 (\n Pfgch<\/jats:italic>\n 1) gene, a key enzyme in the folate biosynthesis pathway, known to play a role in antimalarial (Sulfadoxine-Pyrimethamine, commonly used in combination therapies) resistance. An increase in\n Pfgch<\/jats:italic>\n 1 copy number is hypothesized to compensate for the mutations in dihydrofolate reductase (\n Pfdhfr<\/jats:italic>\n ) and dihydropteroate synthetase (\n Pfdhps<\/jats:italic>\n ) genes associated with Sulfadoxine-Pyrimethamine resistance.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n We analyzed\n Pfgch1<\/jats:italic>\n gene copy number variation (CNV) in one hundred one\n P. falciparum<\/jats:italic>\n isolates collected from Northeast India, using quantitative PCR and the findings of the CNV were used to determine the association with drug resistance genes\n Pfdhfr<\/jats:italic>\n and\n Pfdhps<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The analysis revealed a significant variation in the\n Pfgch1<\/jats:italic>\n gene copy number among the isolates, with most isolates carrying a single copy. Notably, higher copy numbers were significantly associated with the presence of mutations in the\n Pfdhfr<\/jats:italic>\n and\n Pfdhps<\/jats:italic>\n genes.\n <\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusion:<\/jats:title>\n \n A statistically significant positive correlation was observed between mutations in the\n Pfdhfr<\/jats:italic>\n and\n Pfdhps<\/jats:italic>\n genes and increased CNVs in the\n Pfgch<\/jats:italic>\n 1 gene. Further investigation is needed to elucidate the correlation between\n Pfgch<\/jats:italic>\n 1 amplification and how\n Pfdhfr\/Pfdhps<\/jats:italic>\n mutations contribute to antimalarial drug resistance in\n P. falciparum<\/jats:italic>\n . This research finding may help to detect and mitigate the spread of antifolate-resistant\n P. falciparum<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jvbd.jvbd_200_25","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T06:00:15Z","timestamp":1773208815000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Plasmodium falciparum GTP-cyclohydrolase 1 (Pfgch1) Gene Copy Number Variation and Its Implications in antimalarial resistance from Northeast India"],"prefix":"10.4103","author":[{"given":"Aparajita","family":"Sen","sequence":"first","affiliation":[{"name":"ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India"},{"name":"Department of Genetics, University of Delhi, South Campus, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka","family":"Sharma","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India"},{"name":"AcSIR - Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supriya","family":"Sharma","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bina","family":"Srivastava","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuldeep","family":"Singh","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India"},{"name":"AcSIR - Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anupkumar","family":"Anvikar","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India"},{"name":"AcSIR - Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen Kumar","family":"Bharti","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India"},{"name":"AcSIR - Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,3,11]]},"container-title":["Journal of Vector Borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jvbd.jvbd_200_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:39:47Z","timestamp":1787067587000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jvbd.jvbd_200_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.4103\/jvbd.jvbd_200_25","relation":{},"ISSN":["0972-9062"],"issn-type":[{"value":"0972-9062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,11]]},"assertion":[{"value":"2025-07-19","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-06","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}