{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T16:20:18Z","timestamp":1787847618896,"version":"build-2784847793"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"abstract":"\n Background & objectives<\/jats:title>\n \n Accurate diagnosis is crucial for early malaria treatment and for preventing its transmission. However, efforts to eliminate malaria face significant challenges, including limited treatment options and inadequate diagnostic tools for detecting\n Plasmodium falciparum (Pf)<\/jats:italic>\n infections. This study evaluates\n P. falciparum<\/jats:italic>\n glutamate dehydrogenase (\n pfgdh<\/jats:italic>\n ) as a novel diagnostic target, comparing its performance to established markers-\n pfhrp2, pfhrp3, pfaldolase<\/jats:italic>\n , and\n pfldh<\/jats:italic>\n for detecting\n Pf<\/jats:italic>\n infections.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n A retrospective analysis of stored DNA samples was conducted using 150\n Pf-positive<\/jats:italic>\n samples and 50 malaria-negative samples, which were analysed by quantitative real-time polymerase chain reaction (qPCR). Sensitivity, Specificity, positive predictive value, negative 2 predictive value, and the area under the receiver operating characteristic (ROC) curve (AUC) were calculated for each gene, using a threshold cycle value of \u226437 as the cutoff.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Sensitivities of\n pfhrp2, pfhrp3, pfgdh, pfaldolase<\/jats:italic>\n , and\n pfldh<\/jats:italic>\n were 72%, 71%, 69%, 29%, and 19%, respectively, while their specificities were 100%, 96%, 98%, 100%, and 100%, respectively. The AUC values, reflecting diagnostic predictivity, were 86%, 84%, 83%, 64%, and 60%, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusion:<\/jats:title>\n \n These findings suggest that\n pfgdh<\/jats:italic>\n demonstrates notable diagnostic potential compared to the other genes. Its reliability as a target could help overcome challenges posed by\n pfhrp2<\/jats:italic>\n gene deletions, making\n pfgdh<\/jats:italic>\n a promising alternative for detecting\n Pf<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/jvbd.jvbd_276_25","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T08:00:09Z","timestamp":1780041609000},"update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Comparative evaluation of Plasmodium falciparum glutamate dehydrogenase with known Plasmodium falciparum diagnostic targets using quantitative real-time polymerase chain reaction"],"prefix":"10.4103","author":[{"given":"Amreen","family":"Ahmad","sequence":"first","affiliation":[{"name":"Division of Vector Borne Diseases, ICMR-National Institute of Research in Tribal Health, Jabalpur, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrikant","family":"Nema","sequence":"additional","affiliation":[{"name":"Molecular Epidemiology Department, ICMR-National Institute of Malaria Research, New Delhi, India"},{"name":"Malaria Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sri","family":"Krishna","sequence":"additional","affiliation":[{"name":"Division of Vector Borne Diseases, ICMR-National Institute of Research in Tribal Health, Jabalpur, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Himanshu","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Institute of Applied Sciences and Humanities, GLA University, Mathura, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akansha","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Vector Borne Diseases, ICMR-National Institute of Research in Tribal Health, Jabalpur, Madhya Pradesh, India"},{"name":"Molecular Epidemiology Department, ICMR-National Institute of Malaria Research, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil Kumar","family":"Verma","sequence":"additional","affiliation":[{"name":"Division of Vector Borne Diseases, ICMR-National Institute of Research in Tribal Health, Jabalpur, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mrigendra Pal","family":"Singh","sequence":"additional","affiliation":[{"name":"Molecular Epidemiology Department, ICMR-National Institute of Malaria Research, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anup R","family":"Anvikar","sequence":"additional","affiliation":[{"name":"Molecular Epidemiology Department, ICMR-National Institute of Malaria Research, New Delhi, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen Kumar","family":"Bharti","sequence":"additional","affiliation":[{"name":"Division of Vector Borne Diseases, ICMR-National Institute of Research in Tribal Health, Jabalpur, Madhya Pradesh, India"},{"name":"Molecular Epidemiology Department, ICMR-National Institute of Malaria Research, New Delhi, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2026,5,27]]},"container-title":["Journal of Vector Borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/jvbd.jvbd_276_25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:24:15Z","timestamp":1787844255000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/jvbd.jvbd_276_25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,27]]},"references-count":0,"URL":"https:\/\/doi.org\/10.4103\/jvbd.jvbd_276_25","relation":{},"ISSN":["0972-9062"],"issn-type":[{"value":"0972-9062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,27]]},"assertion":[{"value":"2025-09-16","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-12","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}